FastQCFastQC Report
Wed 25 May 2016
SRR1294808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294808_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136524
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC87016.373238404969089No Hit
GTACATGGGAAGCAGTGGTATCAAC85216.241393454630688No Hit
GAGTACATGGGAAGCAGTGGTATCA45753.3510591544343855No Hit
CATGTACTCTGCGTTGATACCACTG42033.0785795904016875No Hit
ACGCAGAGTACATGGGAAGCAGTGG31492.3065541589757115No Hit
GCGTTGATACCACTGCTTCCCATGT30682.2472239313234303No Hit
ACTCTGCGTTGATACCACTGCTTCC27211.9930561659488442No Hit
GTATCAACGCAGAGTACATGGGAAG26641.95130526500835No Hit
TATCAACGCAGAGTACATGGGAAGC25911.897834812926665No Hit
GGTATCAACGCAGAGTACATGGGAA25561.8721982948053089No Hit
GTATCAACGCAGAGTACTTTTTTTT22671.660513902317541No Hit
GTGGTATCAACGCAGAGTACATGGG18941.3873018663385193No Hit
GGTATCAACGCAGAGTACTTTTTTT17551.2854882657994198No Hit
GCTTCCCATGTACTCTGCGTTGATA17241.2627816354633619No Hit
GATACCACTGCTTCCCATGTACTCT15801.15730567519264No Hit
GTACTCTGCGTTGATACCACTGCTT15251.117019718144795No Hit
ATACCACTGCTTCCCATGTACTCTG15011.0994403914330082No Hit
GGGAAGCAGTGGTATCAACGCAGAG14991.0979754475403594No Hit
CAGTGGTATCAACGCAGAGTACATG14001.025460724854238No Hit
CATGGGAAGCAGTGGTATCAACGCA13681.0020216225718555No Hit
TATCAACGCAGAGTACTTTTTTTTT13010.9529460021681169No Hit
CTGCTTCCCATGTACTCTGCGTTGA12420.9097301573349741No Hit
ACATGGGAAGCAGTGGTATCAACGC12220.895080718408485No Hit
GCAGTGGTATCAACGCAGAGTACAT11900.8716416161261022No Hit
TACCACTGCTTCCCATGTACTCTGC11480.8408777943804753No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11300.8276932993466349No Hit
GCAGAGTACATGGGAAGCAGTGGTA11250.8240309396150127No Hit
CCATGTACTCTGCGTTGATACCACT11130.8152412762591192No Hit
GTTGATACCACTGCTTCCCATGTAC10530.7712929594796519No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10260.7515162169288916No Hit
CTTCCCATGTACTCTGCGTTGATAC9770.7156250915589933No Hit
ATGGGAAGCAGTGGTATCAACGCAG9540.6987782367935308No Hit
AAGCAGTGGTATCAACGCAGAGTAC9460.6929184612229352No Hit
CCACTGCTTCCCATGTACTCTGCGT8690.6365181213559521No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8580.628460929946383No Hit
TGATACCACTGCTTCCCATGTACTC7250.5310421610852305No Hit
TTCCCATGTACTCTGCGTTGATACC7080.5185901379977147No Hit
ATCAACGCAGAGTACATGGGAAGCA6770.49588350766165656No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6340.46438721396970495No Hit
GTACATGGTAAGCAGTGGTATCAAC5790.4241012569218599No Hit
ACCATGTACTCTGCGTTGATACCAC4700.34426181477249423No Hit
GGAAGCAGTGGTATCAACGCAGAGT4490.32887990389968064No Hit
GTGGTATCAACGCAGAGTACTTTTT4320.3164278808121649No Hit
TCAACGCAGAGTACATGGGAAGCAG4080.29884855410037797No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3860.2827341712812399No Hit
CTCTGCGTTGATACCACTGCTTCCC3810.2790718115496177No Hit
TGGGAAGCAGTGGTATCAACGCAGA3800.2783393396032932No Hit
AAAAAGTACTCTGCGTTGATACCAC3610.26442237262312857No Hit
CTGCGTTGATACCACTGCTTCCCAT3500.2563651812135595No Hit
TCCCATGTACTCTGCGTTGATACCA3220.23585596671647477No Hit
CCCCATGTACTCTGCGTTGATACCA2930.21461428027306556No Hit
TCCATGTACTCTGCGTTGATACCAC2880.21095192054144327No Hit
AACGCAGAGTACATGGGAAGCAGTG2880.21095192054144327No Hit
GGTAAGCAGTGGTATCAACGCAGAG2840.20802203275614545No Hit
ATGTACTCTGCGTTGATACCACTGC2770.20289472913187426No Hit
AAAGTACTCTGCGTTGATACCACTG2690.1970349535612786No Hit
TTGATACCACTGCTTCCCATGTACT2600.1904427060443585No Hit
ATCAACGCAGAGTACTTTTTTTTTT2470.18092057074214057No Hit
AGTGGTATCAACGCAGAGTACATGG2460.18018809879581613No Hit
GAGTACATGGTAAGCAGTGGTATCA2330.1706659634935982No Hit
CAACGCAGAGTACATGGGAAGCAGT2300.16846854765462482No Hit
GTACATGGAAAGCAGTGGTATCAAC2230.16334124403035363No Hit
GTACATGGGTAAGCAGTGGTATCAA2110.15455158067446015No Hit
ACTGCTTCCCATGTACTCTGCGTTG2040.14942427705018899No Hit
GCTTACCATGTACTCTGCGTTGATA1990.1457619173185667No Hit
CGTTGATACCACTGCTTCCCATGTA1940.14209955758694442No Hit
CGCAGAGTACATGGGAAGCAGTGGT1930.14136708564061995No Hit
ACTCTGCGTTGATACCACTGCTTAC1760.1289150625531042No Hit
GCGTTGATACCACTGCTTACCATGT1720.1259851747678064No Hit
TGTACTCTGCGTTGATACCACTGCT1670.12232281503618411No Hit
ACGCAGAGTACATGGTAAGCAGTGG1590.11646303946558846No Hit
GAGTACATGGAAAGCAGTGGTATCA1590.11646303946558846No Hit
CAGAGTACATGGGAAGCAGTGGTAT1470.107673376109695No Hit
GTATCAACGCAGAGTACATGGTAAG1450.1062084322170461No Hit
TATCAACGCAGAGTACATGGTAAGC1440.10547596027072163No Hit
GCTTTCCATGTACTCTGCGTTGATA1420.10401101637807272No Hit
CTGCTTACCATGTACTCTGCGTTGA1370.10034865664645044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA250.005884229719.0666181
AAAAAGT700.001433791110.8952091
AAAAGTA800.00435678859.5298012
AACAAAA1250.00241360427.6238412
CCATGTA27750.07.2804252
ACCATGT1854.139995E-57.21439551
TGCGTTG29950.07.06445512
TACTCTG30200.06.97711377
CTGCGTT30200.06.97455811
GCTTACC1400.0072557496.8095061
TCTGCGT30900.06.78585310
TTGATAC31200.06.78142416
CTCTGCG31200.06.75101389
TGATACC31500.06.746959717
TGTACTC30000.06.73439265
ATGTACT30300.06.66771554
GTACTCT31700.06.6494036
CCCATGT27250.06.6470781
GATACCA31850.06.64302818
CATGTAC31150.06.5163653
Produced by FastQC (version 0.11.5)