FastQCFastQC Report
Wed 25 May 2016
SRR1294807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231663
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24400.10933550450941741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.012.7169151
AGACCGT752.0806039E-411.3964436
GGCGAGT750.00265372110.13085513
CGAATTA856.625211E-410.05387915
ATAAGAC1803.947207E-1010.026453
GTATCAA24650.09.5571881
CGCCACG800.00437062239.53794219
TTATACG800.00451331039.5008764
CGGGACC800.00451331039.5008764
TAGGACC1304.234833E-69.5008764
TATCCCG900.00111297249.5008765
GGGTTAC1201.7021961E-59.4993831
GGTCTAG1606.644041E-89.4993821
TCTAGAC2002.6557245E-109.4987423
ACGCATT900.00111847249.49575717
GTCTAAG1752.6731868E-89.2279721
GTTTAGG2401.0913936E-119.1035751
TGGACGG1151.1097459E-49.0877955
TAACGCA1151.1168258E-49.08249115
CCGGGTT1054.5135024E-49.04357817
Produced by FastQC (version 0.11.5)