FastQCFastQC Report
Wed 25 May 2016
SRR1294807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231663
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87820.39351819696791135No Hit
GGTATCAACGCAGAGTACTTTTTTT54000.24197201817657954No Hit
TATCAACGCAGAGTACTTTTTTTTT40290.18053801133952574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG250.006078070518.97546810
AGACCGT801.2987039E-714.2414926
AACCGTA400.0053215514.2331957
GGTATCA14850.013.2218671
AGTACCG802.7752969E-511.9130495
TACGTTC600.00573871311.1188465
GTATCAA34850.011.0491941
GCGCAGC1355.4224074E-810.5903311
GCTGCGC1201.4630368E-610.3246433
CTGGACG1701.4752004E-910.0910534
TTAACGC951.5923037E-410.0320413
TTAGAAC2053.8198777E-119.7628893
GGACAGT2450.09.6880896
CTAGAAC2600.09.5304383
AATGCGT1106.8830894E-59.4943286
CGTAGGC900.00112599949.48879618
GACGGAT800.0045609259.4887967
GACTGTC1901.0750227E-99.4887967
AAACGTA1106.9322035E-59.48815915
ACCGTGT1401.0776985E-69.4877338
Produced by FastQC (version 0.11.5)