FastQCFastQC Report
Wed 25 May 2016
SRR1294806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737182
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC49090.2825840930886919No Hit
CCCATGTACTCTGCGTTGATACCAC46270.2663509062378035No Hit
GAGTACATGGGAAGCAGTGGTATCA24890.14327802153142272No Hit
CATGTACTCTGCGTTGATACCACTG20630.11875554777795302No Hit
GCGTTGATACCACTGCTTCCCATGT18890.10873932610400061No Hit
GTATCAACGCAGAGTACATGGGAAG18180.10465224714508901No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG402.755916E-416.6292925
TATACGT400.005269386314.254094
AATCGGA500.001506873313.29154517
ACACCGT802.0115058E-613.05576
CTCGTTC701.0939979E-412.21074512
GCTCGTT701.0939979E-412.21074511
TTTACAC1803.6379788E-1211.08587553
ATCGGAG600.005897672411.07788418
GACCGTC951.3647132E-510.99681211
GACTGTG3750.010.890497
CTAGTAC1158.7908484E-710.7415933
TAGACTG2850.010.6694215
GGACTGT3100.010.4139326
TATACTG2850.010.3360015
GTATAGG2500.010.2623531
GGACCAT1303.8685357E-710.2254446
ATTAGAC1502.435445E-810.1356573
GACTGTC1606.197297E-910.0911147
TCGTTCA856.6113594E-410.05590713
TACACAG4550.010.0245665
Produced by FastQC (version 0.11.5)