FastQCFastQC Report
Wed 25 May 2016
SRR1294806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737182
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53490.30791246973546815No Hit
GTACATGGGAAGCAGTGGTATCAAC50560.2910460734684103No Hit
CCCATGTACTCTGCGTTGATACCAC46010.2648542294359486No Hit
GGTATCAACGCAGAGTACTTTTTTT35690.20544767330078254No Hit
GAGTACATGGGAAGCAGTGGTATCA27450.15801453158045617No Hit
TATCAACGCAGAGTACTTTTTTTTT24530.1412056998057774No Hit
CATGTACTCTGCGTTGATACCACTG20870.12013709559504991No Hit
GTATCAACGCAGAGTACATGGGAAG18790.10816368118021025No Hit
GCGTTGATACCACTGCTTCCCATGT18590.1070123913326295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG508.474193E-515.2499841
TTCGCCG400.005320204414.23348416
ATCCGTC500.001512176513.2853517
GGAACGT751.496997E-512.65162318
GTCCTAT1251.7451384E-811.4374891
GTAACCC752.0155673E-411.4351743
TTAGACA1850.011.3321544
GACACCG600.00592960311.0698528
ATCGTCC600.00592960311.0698528
ATTAGAC1451.3755198E-810.5151033
TTACCCT1658.8039087E-1010.3956124
GTGTAGG2700.09.884251
TAACACT2350.09.7320624
GTATTAA2303.6379788E-129.531241
GTCTTAG1201.6353848E-59.531241
GTCTAAC800.0043952199.53123951
GTGCTAG1909.895302E-109.53123951
TAATACC1803.963578E-99.5293114
TAGAACT1803.963578E-99.5293114
GAACGTC800.0045387759.49418219
Produced by FastQC (version 0.11.5)