FastQCFastQC Report
Wed 25 May 2016
SRR1294805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130701
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC82766.332009701532505No Hit
GTACATGGGAAGCAGTGGTATCAAC81996.273096609819359No Hit
GAGTACATGGGAAGCAGTGGTATCA41953.2096158407357254No Hit
CATGTACTCTGCGTTGATACCACTG40173.073427135217022No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA32702.50189363509078No Hit
GCGTTGATACCACTGCTTCCCATGT30502.3335705159103606No Hit
ACGCAGAGTACATGGGAAGCAGTGG27832.129287457632306No Hit
ACTCTGCGTTGATACCACTGCTTCC26432.0221727454265843No Hit
GTATCAACGCAGAGTACATGGGAAG24691.8890444602566163No Hit
TATCAACGCAGAGTACATGGGAAGC23761.8178896871485297No Hit
GGTATCAACGCAGAGTACATGGGAA23381.7888156938355482No Hit
GTGGTATCAACGCAGAGTACATGGG17541.3419943229202531No Hit
GTACTCTGCGTTGATACCACTGCTT16511.2631884989403295No Hit
GCTTCCCATGTACTCTGCGTTGATA15621.1950941461809779No Hit
ATACCACTGCTTCCCATGTACTCTG15491.1851477800475896No Hit
GATACCACTGCTTCCCATGTACTCT14721.1262346883344427No Hit
GGGAAGCAGTGGTATCAACGCAGAG13111.003052769297863No Hit
CTGCTTCCCATGTACTCTGCGTTGA13091.0015225591234955No Hit
CAGTGGTATCAACGCAGAGTACATG12150.9296026809282255No Hit
CATGGGAAGCAGTGGTATCAACGCA11750.8989984774408766No Hit
GTTGATACCACTGCTTCCCATGTAC10890.8331994399430762No Hit
CCATGTACTCTGCGTTGATACCACT10890.8331994399430762No Hit
ACATGGGAAGCAGTGGTATCAACGC10550.8071858669788295No Hit
TACCACTGCTTCCCATGTACTCTGC10310.78882334488642No Hit
GCAGAGTACATGGGAAGCAGTGGTA9970.7628097719221736No Hit
GCAGTGGTATCAACGCAGAGTACAT9890.7566889312247037No Hit
CCACTGCTTCCCATGTACTCTGCGT9690.7413868294810292No Hit
CTTCCCATGTACTCTGCGTTGATAC9320.7130779412552314No Hit
ATGGGAAGCAGTGGTATCAACGCAG9200.7038966802090267No Hit
AAGCAGTGGTATCAACGCAGAGTAC7900.6044330188751424No Hit
TTCCCATGTACTCTGCGTTGATACC7690.5883658120442843No Hit
GTATCAACGCAGAGTACTTTTTTTT7500.5738288153877935No Hit
TGATACCACTGCTTCCCATGTACTC7240.5539360831210167No Hit
ATCAACGCAGAGTACATGGGAAGCA5990.45829794722305106No Hit
GGTATCAACGCAGAGTACTTTTTTT5020.38408275376622975No Hit
GTACATGGTAAGCAGTGGTATCAAC4840.3703108621969228No Hit
ACCATGTACTCTGCGTTGATACCAC4660.3565389706276157No Hit
TATCAACGCAGAGTACTTTTTTTTT4220.3228743467915318No Hit
GGAAGCAGTGGTATCAACGCAGAGT3970.3037467196119387No Hit
CTGCGTTGATACCACTGCTTCCCAT3600.27543783138614086No Hit
CTCTGCGTTGATACCACTGCTTCCC3590.27467272629895717No Hit
TGGGAAGCAGTGGTATCAACGCAGA3450.263961255078385No Hit
AAACAAAAAAAAAAAAAAAAAAAAA3200.2448336278987919No Hit
TCAACGCAGAGTACATGGGAAGCAG3120.23871278720132208No Hit
AAAAAGTACTCTGCGTTGATACCAC3110.2379476821141384No Hit
TCCCATGTACTCTGCGTTGATACCA2790.2134643193242592No Hit
ATGTACTCTGCGTTGATACCACTGC2760.211169004062708No Hit
CCCCATGTACTCTGCGTTGATACCA2710.2073434786267894No Hit
GGTAAGCAGTGGTATCAACGCAGAG2630.2012226379293196No Hit
AAAGTACTCTGCGTTGATACCACTG2620.20045753284213588No Hit
TCCATGTACTCTGCGTTGATACCAC2600.1989273226677684No Hit
TTGATACCACTGCTTCCCATGTACT2530.19357158705748234No Hit
AACGCAGAGTACATGGGAAGCAGTG2390.1828601158369102No Hit
ACTGCTTCCCATGTACTCTGCGTTG2380.18209501074972648No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2380.18209501074972648No Hit
AGTGGTATCAACGCAGAGTACATGG2280.17444395987788924No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2220.1698533293547869No Hit
GAGTACATGGTAAGCAGTGGTATCA2200.16832311918041942No Hit
GTACATGGAAAGCAGTGGTATCAAC2000.15302101743674495No Hit
CAACGCAGAGTACATGGGAAGCAGT1960.14996059708801004No Hit
GCTTACCATGTACTCTGCGTTGATA1820.13924912586743787No Hit
GTACATGGGTAAGCAGTGGTATCAA1780.136188705518703No Hit
AAAAACAAAAAAAAAAAAAAAAAAA1770.13542360043151927No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1650.12624233938531457No Hit
TGTACTCTGCGTTGATACCACTGCT1610.12318191903657968No Hit
CGTTGATACCACTGCTTCCCATGTA1500.1147657630775587No Hit
CGCAGAGTACATGGGAAGCAGTGGT1490.11400065799037498No Hit
CTGCTTACCATGTACTCTGCGTTGA1460.1117053427288238No Hit
GAGTACATGGAAAGCAGTGGTATCA1440.11017513255445635No Hit
ACTCTGCGTTGATACCACTGCTTAC1440.11017513255445635No Hit
GCTTTCCATGTACTCTGCGTTGATA1390.10634960711853773No Hit
GCGTTGATACCACTGCTTACCATGT1390.10634960711853773No Hit
ACGCAGAGTACATGGTAAGCAGTGG1370.10481939694417029No Hit
GAACAAAAAAAAAAAAAAAAAAAAA1310.10022876642106794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA1050.017.1904771
GAAAAAA2002.4920155E-109.51
ACAAAAA5900.08.6949163
AACAAAA4000.08.31252
CAAAAAA6150.07.87804844
AATCAAC1100.00647116647.811635519
CACAAAA1502.2629634E-47.60000042
ACACAAA1553.302536E-47.3548391
GAAGCAG24500.07.01567949
GGAAGCA24850.06.9168678
CCATGTA25550.06.91585162
AGTGGTA27400.06.793017414
CAGTGGT27750.06.707340213
GGGAAGC25800.06.66217667
TGCGTTG26950.06.65978612
CTGCGTT27250.06.586467311
GTGGTAT28450.06.508929715
TACTCTG27750.06.5020137
TGGTATC28350.06.49839216
AGCAGTG28550.06.486131711
Produced by FastQC (version 0.11.5)