FastQCFastQC Report
Wed 25 May 2016
SRR1294805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130701
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC85836.56689696329791No Hit
CCCATGTACTCTGCGTTGATACCAC81916.266975769121889No Hit
GAGTACATGGGAAGCAGTGGTATCA46053.5233089264810524No Hit
CATGTACTCTGCGTTGATACCACTG40833.1239240709711478No Hit
ACGCAGAGTACATGGGAAGCAGTGG30622.342751776956565No Hit
GCGTTGATACCACTGCTTCCCATGT29942.2907246310280716No Hit
GTATCAACGCAGAGTACATGGGAAG26592.034414426821524No Hit
TATCAACGCAGAGTACATGGGAAGC26081.995394067375154No Hit
ACTCTGCGTTGATACCACTGCTTCC26001.9892732266776842No Hit
GGTATCAACGCAGAGTACATGGGAA25031.9150580332208629No Hit
GTATCAACGCAGAGTACTTTTTTTT20861.5960092118652496No Hit
GTGGTATCAACGCAGAGTACATGGG17851.3657125806229484No Hit
GGTATCAACGCAGAGTACTTTTTTT15671.1989196716168964No Hit
GCTTCCCATGTACTCTGCGTTGATA15611.1943290410937941No Hit
GATACCACTGCTTCCCATGTACTCT14551.1132279018523195No Hit
ATACCACTGCTTCCCATGTACTCTG14151.0826236983649704No Hit
GTACTCTGCGTTGATACCACTGCTT13971.0688518067956634No Hit
CATGGGAAGCAGTGGTATCAACGCA13901.0634960711853774No Hit
GGGAAGCAGTGGTATCAACGCAGAG13601.0405429185698656No Hit
TATCAACGCAGAGTACTTTTTTTTT13101.0022876642106793No Hit
CAGTGGTATCAACGCAGAGTACATG12980.9931064031644745No Hit
ACATGGGAAGCAGTGGTATCAACGC11740.8982333723536928No Hit
GCAGAGTACATGGGAAGCAGTGGTA11370.8699244841278949No Hit
GCAGTGGTATCAACGCAGAGTACAT11360.8691593790407113No Hit
CTGCTTCCCATGTACTCTGCGTTGA11150.853092172209853No Hit
TACCACTGCTTCCCATGTACTCTGC11020.8431458060764646No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11010.8423807009892809No Hit
CCATGTACTCTGCGTTGATACCACT10720.8201926534609528No Hit
GTTGATACCACTGCTTCCCATGTAC10380.7941790804967063No Hit
CCACTGCTTCCCATGTACTCTGCGT9160.7008362598602917No Hit
CTTCCCATGTACTCTGCGTTGATAC9160.7008362598602917No Hit
ATGGGAAGCAGTGGTATCAACGCAG9130.6985409445987406No Hit
AAGCAGTGGTATCAACGCAGAGTAC9050.6924201039012708No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8850.6771180021575963No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8030.614379385008531No Hit
TGATACCACTGCTTCCCATGTACTC7120.544754822074812No Hit
TTCCCATGTACTCTGCGTTGATACC6780.5187412491105653No Hit
ATCAACGCAGAGTACATGGGAAGCA6480.49578809649505357No Hit
GTACATGGTAAGCAGTGGTATCAAC5750.4399354251306417No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5650.43228437425880445No Hit
ACCATGTACTCTGCGTTGATACCAC4650.355773865540432No Hit
GGAAGCAGTGGTATCAACGCAGAGT4430.33894155362239003No Hit
TGGGAAGCAGTGGTATCAACGCAGA3960.30298161452475497No Hit
CTCTGCGTTGATACCACTGCTTCCC3620.27696804156050836No Hit
CTGCGTTGATACCACTGCTTCCCAT3570.27314251612458973No Hit
TCAACGCAGAGTACATGGGAAGCAG3350.25631020420654776No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3010.2302966312423011No Hit
AAAAAGTACTCTGCGTTGATACCAC2950.22570600071919877No Hit
GTGGTATCAACGCAGAGTACTTTTT2860.2188200549345453No Hit
AAAGTACTCTGCGTTGATACCACTG2740.20963879388834059No Hit
GGTAAGCAGTGGTATCAACGCAGAG2740.20963879388834059No Hit
TTGATACCACTGCTTCCCATGTACT2730.20887368880115684No Hit
AACGCAGAGTACATGGGAAGCAGTG2710.2073434786267894No Hit
TCCCATGTACTCTGCGTTGATACCA2670.20428305827805449No Hit
TCCATGTACTCTGCGTTGATACCAC2550.1951017972318498No Hit
ATGTACTCTGCGTTGATACCACTGC2430.18592053618564508No Hit
GAGTACATGGTAAGCAGTGGTATCA2430.18592053618564508No Hit
CAACGCAGAGTACATGGGAAGCAGT2400.18362522092409392No Hit
ATCAACGCAGAGTACTTTTTTTTTT2350.1797996954881753No Hit
AGTGGTATCAACGCAGAGTACATGG2320.17750438022662413No Hit
CCCCATGTACTCTGCGTTGATACCA2290.17520906496507294No Hit
GTACATGGAAAGCAGTGGTATCAAC2100.1606720683085822No Hit
GCTTACCATGTACTCTGCGTTGATA2030.15531633269829612No Hit
GTACATGGGTAAGCAGTGGTATCAA1980.15149080726237749No Hit
CGCAGAGTACATGGGAAGCAGTGGT1880.14383975639054022No Hit
ACTGCTTCCCATGTACTCTGCGTTG1870.1430746513033565No Hit
CGTTGATACCACTGCTTCCCATGTA1630.12471212921094713No Hit
ACTCTGCGTTGATACCACTGCTTAC1630.12471212921094713No Hit
ACGCAGAGTACATGGTAAGCAGTGG1600.12241681394939595No Hit
ATGGTAAGCAGTGGTATCAACGCAG1520.11629597325192614No Hit
GAGTACATGGAAAGCAGTGGTATCA1440.11017513255445635No Hit
GCGTTGATACCACTGCTTACCATGT1420.10864492238008891No Hit
TGTACTCTGCGTTGATACCACTGCT1390.10634960711853773No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1380.105584502031354No Hit
GTATCAACGCAGAGTACATGGTAAG1380.105584502031354No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1340.1025240816826191No Hit
CAACGCAGAGTACTTTTTTTTTTTT1330.1017589765954354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTC400.0051115414.3053641
GAACAAA400.0051115414.3053641
GAAAAAA1054.2533712E-49.082771
AACAAAA1054.302778E-49.0723072
CATGGTA1606.260161E-78.9305524
ACATGGT1502.5261852E-68.8908613
ATGGTAA1606.0346247E-68.3351815
TGGTAAG1752.0943839E-57.59448866
CCATGTA26900.07.3303562
ACAAAAA1300.00355504247.3276323
TAAGCAG1854.394535E-57.18122779
TGCGTTG29100.06.97851812
CTGCGTT29300.06.93088311
CCCATGT26200.06.9160791
TACTCTG29550.06.87224637
CAGTGGT25850.06.828020613
GTAAGCA1958.780352E-56.8129598
AGTGGTA26100.06.798976414
CTCTGCG30050.06.7578999
TCTGCGT30200.06.72433410
Produced by FastQC (version 0.11.5)