##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294805_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130701
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14103182072058	33.0	31.0	34.0	30.0	34.0
2	32.2544203946412	33.0	31.0	34.0	30.0	34.0
3	32.42607172095087	34.0	31.0	34.0	30.0	34.0
4	35.73952762411917	37.0	35.0	37.0	35.0	37.0
5	35.83769825785571	37.0	35.0	37.0	35.0	37.0
6	35.71154008002999	37.0	37.0	37.0	35.0	37.0
7	35.78932066319309	37.0	37.0	37.0	35.0	37.0
8	35.80593874568672	37.0	37.0	37.0	35.0	37.0
9	37.57583339071621	39.0	39.0	39.0	35.0	39.0
10	37.62523622619567	39.0	39.0	39.0	35.0	39.0
11	37.64254290326777	39.0	39.0	39.0	35.0	39.0
12	37.66368275682665	39.0	39.0	39.0	35.0	39.0
13	37.535611816282966	39.0	39.0	39.0	35.0	39.0
14	39.020191123250775	41.0	40.0	41.0	36.0	41.0
15	38.964843421243906	41.0	39.0	41.0	36.0	41.0
16	38.83859343080772	41.0	39.0	41.0	36.0	41.0
17	38.979357464747785	41.0	39.0	41.0	36.0	41.0
18	38.97338964506775	41.0	39.0	41.0	36.0	41.0
19	38.90748349285774	41.0	39.0	41.0	35.0	41.0
20	39.00021422942441	41.0	39.0	41.0	36.0	41.0
21	38.93354297212722	41.0	39.0	41.0	35.0	41.0
22	39.020910322032734	41.0	39.0	41.0	35.0	41.0
23	38.968844920849875	41.0	39.0	41.0	35.0	41.0
24	38.97155339285851	41.0	39.0	41.0	35.0	41.0
25	38.185530332591185	41.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1275809724268413
1101	2	0.14342950547213462
1101	3	0.21591085074120997
1101	4	0.15208258935431473
1101	5	-0.165610995249466
1101	6	0.19521690744413434
1101	7	0.263984177103346
1101	8	0.34007071628979446
1101	9	0.27128342732500954
1101	10	0.44123297216832924
1101	11	0.2884366732862773
1101	12	0.461480509910956
1101	13	0.4283425821891029
1101	14	0.30082551259541646
1101	15	0.3900056043551601
1101	16	0.3170403391081038
1101	17	0.3643484028811059
1101	18	0.5241120227354301
1101	19	0.28433447452661653
1101	20	0.45248505638726044
1101	21	0.38120579429819657
1101	22	0.27590573355507786
1101	23	0.07136226797522482
1101	24	0.32700992967095033
1101	25	0.019131206910813603
1102	1	0.3365074253632159
1102	2	0.3085218835390293
1102	3	0.12876061014238616
1102	4	0.38026532564612125
1102	5	0.15099923465607645
1102	6	0.2970704094936778
1102	7	0.22037935324544833
1102	8	0.2998818016578184
1102	9	0.4600495415054837
1102	10	0.42165832712585427
1102	11	0.21438570197991424
1102	12	0.3766471468740633
1102	13	0.21765524184572627
1102	14	0.5909104647726835
1102	15	0.7977463516966949
1102	16	0.6323733159987341
1102	17	0.6786120516662351
1102	18	0.5880934877149357
1102	19	0.731075994160669
1102	20	0.525436996029633
1102	21	0.43401872128684715
1102	22	0.4562360371254428
1102	23	0.6307099778308682
1102	24	0.49539940272982363
1102	25	0.9411593658842534
1103	1	0.06337380144250204
1103	2	-0.021859304167456628
1103	3	0.31446815338345147
1103	4	0.23659115368132433
1103	5	0.11782230026033602
1103	6	0.579476917170183
1103	7	0.4686094540255894
1103	8	0.5567184711933422
1103	9	0.48820135027308
1103	10	0.5407898965753049
1103	11	0.8615441461343849
1103	12	0.6389809151630175
1103	13	0.7265810865388076
1103	14	0.7585442136274523
1103	15	0.6428558772248891
1103	16	0.7050016510441282
1103	17	0.8322254223872392
1103	18	0.8471789703768664
1103	19	0.9213366899045852
1103	20	0.9362479569113873
1103	21	0.7025436664546802
1103	22	0.6514663322474661
1103	23	0.8119198948191197
1103	24	0.7348285928794667
1103	25	0.9673399927756208
1104	1	-0.059518145201707284
1104	2	-0.24338578473784978
1104	3	0.14143018869087598
1104	4	-0.24739948011593071
1104	5	0.2673395198662192
1104	6	0.03586882435006089
1104	7	0.04876152327234706
1104	8	0.04307696523422777
1104	9	0.040044334829367756
1104	10	0.1822958556902563
1104	11	0.054945708922147674
1104	12	-0.1073423240697906
1104	13	-0.021215487956709467
1104	14	0.3924204008462695
1104	15	0.06120416315716426
1104	16	0.12816458613844617
1104	17	0.1921927985349967
1104	18	-0.18049868491530674
1104	19	-0.044776599053300004
1104	20	0.215813323008895
1104	21	-0.024863000387384204
1104	22	-0.18817691014249505
1104	23	0.15386522355705523
1104	24	0.3349759264057326
1104	25	0.3443296531192246
1105	1	-0.5063688512147202
1105	2	-0.429884277519065
1105	3	-0.35854336887430094
1105	4	-0.4303546866313468
1105	5	-0.3168003077291033
1105	6	-0.22218289705244842
1105	7	-0.20196564368518466
1105	8	-0.22933299827521125
1105	9	-0.33477717873559953
1105	10	-0.3067305338596924
1105	11	-0.3985949331001706
1105	12	-0.49583457643639406
1105	13	-0.5195231721282667
1105	14	-0.7729770289490645
1105	15	-0.9500074292062592
1105	16	-0.771750798070137
1105	17	-0.8756320467767651
1105	18	-0.32029983780546445
1105	19	-0.5493246978422306
1105	20	-0.49342566371700514
1105	21	-0.3750666071354942
1105	22	-0.5065967102376874
1105	23	-0.441671293445431
1105	24	-0.22121424758548613
1105	25	-0.5118235014632546
1106	1	-0.4260884351134102
1106	2	-0.6408747795543448
1106	3	-0.4193001908251546
1106	4	-0.4593762455036554
1106	5	-0.5184436063873292
1106	6	-0.5078002647593678
1106	7	-0.27493413456412696
1106	8	-0.12349853423179269
1106	9	-0.4326966721774781
1106	10	-0.23225442953284414
1106	11	-0.5164903047268865
1106	12	-0.11531294218341515
1106	13	-0.4800990804653722
1106	14	-0.4131850796048724
1106	15	-0.5721994991595238
1106	16	-0.2711477526909718
1106	17	-0.4100056333891544
1106	18	-0.3004747209654184
1106	19	-0.9822358994101492
1106	20	-0.6003154571739628
1106	21	-0.6619654462731432
1106	22	-0.9475736437519373
1106	23	-0.7929452268089179
1106	24	-0.3865994428214279
1106	25	-0.47669610812690166
1107	1	0.20236826615183645
1107	2	0.07673008950138893
1107	3	0.058059205088675014
1107	4	-0.012700992327907557
1107	5	0.0055859918145557685
1107	6	0.23197800127088897
1107	7	-0.04686130076358097
1107	8	0.04770130413979956
1107	9	-0.22702452529766504
1107	10	0.2280077115384458
1107	11	-0.03193339384979765
1107	12	-0.09394768723986147
1107	13	-0.19452189027419564
1107	14	-0.35557970546037154
1107	15	-0.3356916829108201
1107	16	-0.15182053093174375
1107	17	-0.09321395725207537
1107	18	-0.22044340546172236
1107	19	-0.43085574434790175
1107	20	-0.24218226526548392
1107	21	-0.24026683274180982
1107	22	0.0697761554752816
1107	23	-0.08869995570252343
1107	24	0.22928054808697595
1107	25	-0.026308452496422774
1108	1	0.0935728737270054
1108	2	0.28631618868139697
1108	3	0.06594186811093294
1108	4	0.31590599712933454
1108	5	-0.04324747167626697
1108	6	0.22638936401981624
1108	7	0.189594185374645
1108	8	-0.035859804802022666
1108	9	0.14948855553013374
1108	10	-0.03478026190942529
1108	11	0.2104279884227509
1108	12	0.12090979030797655
1108	13	0.003301225696127119
1108	14	-0.05313437160640433
1108	15	-0.2538632955621267
1108	16	0.09717888335954683
1108	17	0.04088893224421497
1108	18	0.04163683663121276
1108	19	0.04017607915854171
1108	20	0.20874022594888686
1108	21	0.18957014500125524
1108	22	0.23160259046161258
1108	23	0.11432132504718595
1108	24	0.31015715878591976
1108	25	0.08209646745671506
1109	1	0.33432378947792074
1109	2	0.224228276593486
1109	3	0.06598765565671982
1109	4	0.2347246784480177
1109	5	0.06357487264607897
1109	6	0.0843106094410615
1109	7	0.061526419806874344
1109	8	-0.0289000978423104
1109	9	0.10461676065833814
1109	10	0.05459702746786377
1109	11	0.02146278695754944
1109	12	-0.09612317672499415
1109	13	-0.3638233164284088
1109	14	0.1711788152067797
1109	15	0.14883816963934038
1109	16	0.011510385191051853
1109	17	0.1641947930500791
1109	18	0.2079829904773689
1109	19	0.14562479710725995
1109	20	0.13067246038112046
1109	21	0.37890164683275884
1109	22	-0.19490839854938002
1109	23	0.08487993310579611
1109	24	0.19321576684453134
1109	25	0.20361661397686248
1110	1	-0.6105078016951353
1110	2	-0.6426706781103455
1110	3	-0.27803092738625423
1110	4	-0.36666904977497694
1110	5	-0.3195842446593815
1110	6	-0.5796847447982003
1110	7	-0.2550821748505001
1110	8	-0.37582228134986195
1110	9	-0.2303425889351587
1110	10	-0.6501648772940385
1110	11	-0.4065279215209614
1110	12	-0.2090151627877077
1110	13	-0.03258057542957005
1110	14	-0.21000585378276782
1110	15	-0.033624083554620654
1110	16	-0.35167196091812514
1110	17	-0.26565421972576075
1110	18	-0.09933408269336752
1110	19	-0.01453780451875275
1110	20	-0.20788735378845047
1110	21	-0.1306221626910542
1110	22	0.04063167113703514
1110	23	0.061999561444935125
1110	24	-0.24673777554804133
1110	25	0.39780941304771034
1111	1	-0.18832863597824456
1111	2	-0.031980001110113676
1111	3	-0.19093268257202567
1111	4	-0.40439414665211615
1111	5	-0.37363028979272883
1111	6	-0.3087022966559587
1111	7	-0.2668669936332648
1111	8	-0.25368870487390893
1111	9	-0.6975639246999137
1111	10	-0.28100599878936805
1111	11	-0.2430676981336859
1111	12	-0.3825050191816004
1111	13	-0.3578598095303249
1111	14	-0.16847405528721282
1111	15	0.11857559732069234
1111	16	0.0926407812747172
1111	17	-0.3344255896824535
1111	18	-0.46397962634506484
1111	19	-0.3736898446372834
1111	20	-0.5491228222179032
1111	21	-0.8044539383919869
1111	22	-0.32307929307540917
1111	23	-0.27141735216345353
1111	24	-0.41261422870509534
1111	25	-0.7657647834419166
1112	1	0.24569091697407686
1112	2	0.04819141023403972
1112	3	-6.790110099430535E-4
1112	4	0.11214403328672518
1112	5	0.17770697710076178
1112	6	-0.2863499128323639
1112	7	-0.5040649780425852
1112	8	-0.5239845832493799
1112	9	-0.17956142674565
1112	10	-0.3782754763723801
1112	11	-0.142438902750591
1112	12	-0.1007314716558696
1112	13	-0.11159597387848663
1112	14	-0.06476588525404736
1112	15	-0.21444908074468572
1112	16	-0.1560779404617918
1112	17	-0.40370075226482527
1112	18	-0.13487415237977984
1112	19	0.05238362967254773
1112	20	-0.38468852272180243
1112	21	-0.21633644840533606
1112	22	-0.18569180868762913
1112	23	-0.37333819285426983
1112	24	-0.4848180273182976
1112	25	-0.3195784705085458
1113	1	-0.11869208353795102
1113	2	-0.14402569166048096
1113	3	-0.17591710624804335
1113	4	-0.032576908853570785
1113	5	0.030876459947663193
1113	6	-0.49632431119385956
1113	7	-0.3797434214629618
1113	8	-0.5784239073661226
1113	9	-0.282049906008325
1113	10	-0.43683154396070734
1113	11	-0.12869594320117983
1113	12	-0.3519961925980084
1113	13	-0.35810903071412525
1113	14	-0.9424719784440114
1113	15	-0.2190983382971723
1113	16	-0.6357912021105392
1113	17	-0.6875512386959528
1113	18	-0.7697947873004125
1113	19	-0.66548631400385
1113	20	-0.7661529364442785
1113	21	-0.49062216269105363
1113	22	-0.9542734779144553
1113	23	-0.5148026065767439
1113	24	-0.4220223283935667
1113	25	-0.9316214812612316
1114	1	-0.13223213521753863
1114	2	-0.13989132473541588
1114	3	0.026563513906999958
1114	4	0.0500070704745923
1114	5	0.21495914728616583
1114	6	-0.25932172721453384
1114	7	-0.19007933877562522
1114	8	-0.21904406240488328
1114	9	-0.371352231589718
1114	10	-0.2548160400847337
1114	11	0.02954695085566783
1114	12	-0.1977843062931015
1114	13	-0.44710127877613814
1114	14	-0.21885440738458328
1114	15	-0.1841629377803713
1114	16	-0.24219947084439042
1114	17	-0.021298008721792883
1114	18	-0.25713328859239937
1114	19	-0.08770853622607433
1114	20	-0.039537949364174096
1114	21	-0.13992448827244885
1114	22	-0.12646986034339847
1114	23	0.013672845619637997
1114	24	-0.13147969273465065
1114	25	-0.15828814792789814
1115	1	0.17908569423982357
1115	2	0.14486319722841046
1115	3	0.13519400486307376
1115	4	0.28297864670198436
1115	5	0.45309868216988747
1115	6	0.1881201332505853
1115	7	0.2558121340925936
1115	8	0.35935387041165257
1115	9	0.14017231621389925
1115	10	0.25205734492818266
1115	11	-0.10647372097896124
1115	12	0.15022602962421416
1115	13	0.05522430261920874
1115	14	-2.497562217911309E-4
1115	15	0.14534610614727228
1115	16	-0.013568979888312072
1115	17	0.2813376501929383
1115	18	0.19020521269958834
1115	19	0.06784701932948423
1115	20	0.34495817466683576
1115	21	0.19382228175339122
1115	22	0.1123931887522076
1115	23	0.24964183786769922
1115	24	-0.1242445506157921
1115	25	0.023934074294324148
1116	1	-0.009933913603312305
1116	2	0.0069044239755200465
1116	3	0.2449577052150076
1116	4	0.14254626862254582
1116	5	0.27717108035993476
1116	6	0.06830112872569316
1116	7	-0.3005076245801064
1116	8	-0.1806561848925199
1116	9	0.01713561435366273
1116	10	0.11454100505890352
1116	11	-0.14223038261797427
1116	12	-0.17886396534813542
1116	13	-0.3909295435346394
1116	14	-0.40748957522631457
1116	15	-0.06523207384267238
1116	16	-0.33240256460425144
1116	17	-0.4056608415114411
1116	18	-0.21373646653621137
1116	19	-0.1501519751400977
1116	20	-0.4227642989379916
1116	21	-0.17044116721594094
1116	22	-0.043001481936578045
1116	23	-0.21231894644602534
1116	24	-0.22208266021860368
1116	25	-0.1718628085613858
1201	1	-0.24863360400578927
1201	2	-0.2223882647598927
1201	3	-0.3503030711603188
1201	4	-0.10813246440912394
1201	5	-0.03742763361958623
1201	6	-0.17082723517047071
1201	7	-0.32067909397758143
1201	8	-0.33666952140121253
1201	9	-0.27783937969253714
1201	10	-0.13437540360983036
1201	11	-0.25197079700235037
1201	12	-0.051762274469354
1201	13	-0.4184599079071063
1201	14	-0.11077607201126227
1201	15	0.1350537084864598
1201	16	-0.055908161174862414
1201	17	-0.14357463050881591
1201	18	-0.5183327989963189
1201	19	-0.024550641489227587
1201	20	-0.2410067376138656
1201	21	-0.3148326890068418
1201	22	-0.2678407293764451
1201	23	-0.229656407746333
1201	24	-0.2686889950602307
1201	25	-0.058288147927896716
1202	1	-0.0052405468949245915
1202	2	-0.06802096352809173
1202	3	0.20372828382288333
1202	4	0.13229306750577052
1202	5	0.029481660893289074
1202	6	0.09535417580377725
1202	7	0.15205326884436232
1202	8	0.3438455961799747
1202	9	0.4007160514384793
1202	10	0.4668563993017045
1202	11	0.43291162181063925
1202	12	0.2632993156762211
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2210	3	0.4941945364951792
2210	4	0.4019153824808299
2210	5	0.4888881558540987
2210	6	0.4803689370783317
2210	7	0.4960673175056698
2210	8	0.45328263170883787
2210	9	0.49058167293904376
2210	10	0.5555767495002186
2210	11	0.36429714558138215
2210	12	0.42335734275552994
2210	13	0.6705353074038953
2210	14	0.6696067031083501
2210	15	0.6039532300175594
2210	16	0.23595786753326564
2210	17	0.5669516852806566
2210	18	0.41468155507066484
2210	19	0.6625306982236907
2210	20	0.33698369300814335
2210	21	0.3277510430505757
2210	22	0.43598702181972726
2210	23	0.467951247756055
2210	24	0.638966507332114
2210	25	0.5163530003974586
2211	1	0.2295618847160128
2211	2	0.2813711337363429
2211	3	0.07074956041862634
2211	4	0.3061532498765871
2211	5	0.27309868216988775
2211	6	0.1557391808696309
2211	7	-0.07656881828835793
2211	8	0.10919514025292187
2211	9	0.028426284467862217
2211	10	-0.05492678205594359
2211	11	0.06908183457660044
2211	12	0.318162537560724
2211	13	0.29331954071444244
2211	14	0.06165500568297233
2211	15	0.18693340773457834
2211	16	0.41151038519105043
2211	17	0.278480507335793
2211	18	0.4032210857154581
2211	19	0.31705336853583077
2211	20	0.15924388895254538
2211	21	0.12175878968989906
2211	22	-0.00919411283508964
2211	23	0.027737075962939173
2211	24	0.02178719541595342
2211	25	0.17504518540543756
2212	1	0.47422898232429134
2212	2	0.6449134737492699
2212	3	0.3942789721833293
2212	4	0.6131345039166689
2212	5	0.539614519273961
2212	6	0.5750884590424405
2212	7	0.6497186197637816
2212	8	0.30350671103508375
2212	9	0.46509941525864207
2212	10	0.5896541272308511
2212	11	0.5546021965675507
2212	12	0.4998690663260774
2212	13	0.654319325243641
2212	14	0.5744108772623733
2212	15	0.6089760709537089
2212	16	0.5047015077939392
2212	17	0.5671898372215907
2212	18	0.5011956601606258
2212	19	0.5647801515567323
2212	20	0.4957877372611037
2212	21	0.29109729432252607
2212	22	0.49546005652496206
2212	23	0.48451363273949255
2212	24	0.4340259370664654
2212	25	0.7150150194929168
2213	1	0.5968634720176027
2213	2	0.6026409750061887
2213	3	0.44630511597418376
2213	4	0.5496453133686501
2213	5	0.3864320155032175
2213	6	0.8725645776950302
2213	7	0.4958121340925885
2213	8	0.43935387041165797
2213	9	0.6557278717694501
2213	10	0.3631684560392969
2213	11	0.626859612354373
2213	12	0.26133714073532843
2213	13	0.513002080396987
2213	14	0.6175280215559837
2213	15	0.3453461061472751
2213	16	0.2930976867783528
2213	17	0.4724487613040438
2213	18	0.4524274349218089
2213	19	0.5389581304405979
2213	20	0.6338470635557201
2213	21	0.7671556150867289
2213	22	0.5301709665299867
2213	23	0.34297517120103294
2213	24	0.4179776716064296
2213	25	0.40171185207210414
2214	1	-0.21355382310557403
2214	2	-0.0926430873366968
2214	3	-0.0265355074546747
2214	4	0.07014807857729721
2214	5	0.0690262839798379
2214	6	-0.004097061319555451
2214	7	-0.30503251145793797
2214	8	0.036538385243225946
2214	9	-0.032638141302442136
2214	10	0.17788942134849606
2214	11	-0.26440232831932775
2214	12	-0.13361509656985504
2214	13	-0.28233225846676646
2214	14	-0.29889229015844165
2214	15	-0.49057144035850797
2214	16	-0.7351174967309504
2214	17	-0.2608644614209439
2214	18	-0.33590841223756485
2214	19	-0.48499360409937253
2214	20	-0.3096421269922942
2214	21	-0.2609389047725017
2214	22	-0.2601960520723239
2214	23	-0.15802030391208888
2214	24	-3.632032050333578E-4
2214	25	-0.008966880959569323
2215	1	0.5108118847160128
2215	2	0.5929782765934846
2215	3	0.41896384613291104
2215	4	0.17133182130515934
2215	5	0.521312967884171
2215	6	0.36169156182201334
2215	7	0.4121216579021123
2215	8	0.37348085453864144
2215	9	0.48705723684881974
2215	10	0.4525136941345309
2215	11	0.2128318345765976
2215	12	0.16280539470358235
2215	13	0.4329028740477767
2215	14	0.40153595806392417
2215	15	0.4717548363060047
2215	16	0.43680800423867083
2215	17	0.25139717400245587
2215	18	0.6151258476202202
2215	19	0.6122914637739285
2215	20	0.41341055561921536
2215	21	0.5649135515946639
2215	22	0.3943773157363353
2215	23	0.24618945691531735
2215	24	0.39738243351119706
2215	25	0.41314042350067126
2216	1	0.06019195333735183
2216	2	0.19066620547683044
2216	3	0.05109890539679185
2216	4	-0.022542944758470185
2216	5	0.22550042889477595
2216	6	0.06357864853442408
2216	7	0.2424788007592582
2216	8	0.011503312430093615
2216	9	0.1148059891881772
2216	10	-0.056278414411941924
2216	11	-0.09816707372034728
2216	12	-0.14790109313172906
2216	13	-0.07315997685677189
2216	14	-0.2971929876628394
2216	15	-0.1754496240807697
2216	16	-0.020284166690835548
2216	17	0.022938814676194852
2216	18	0.26562491187474535
2216	19	0.16032639827174222
2216	20	0.17319689373524483
2216	21	0.2746180119814383
2216	22	0.20122385347806926
2216	23	0.2675166559171913
2216	24	0.33698786083495946
2216	25	-0.10588640120300852
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	32.0
10	57.0
11	64.0
12	79.0
13	99.0
14	157.0
15	241.0
16	275.0
17	340.0
18	370.0
19	411.0
20	462.0
21	443.0
22	448.0
23	408.0
24	423.0
25	445.0
26	416.0
27	435.0
28	415.0
29	514.0
30	563.0
31	806.0
32	1037.0
33	1600.0
34	2341.0
35	3788.0
36	6714.0
37	16565.0
38	90746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.6697070796731	19.436962443757462	11.599736769612194	23.293593706957243
2	11.90698457394711	26.0674277668952	32.488828354554364	29.536759304603333
3	24.269133365467745	31.231589658839642	27.09007582191414	17.409201153778472
4	15.347242943818335	22.48261298689375	27.380815755043958	34.78932831424396
5	10.987077641708025	39.68055192759792	34.631459258462534	14.700911172231526
6	32.13801866068627	28.54442054665554	18.41039732412801	20.907163468530186
7	19.21770269029474	36.315918342362195	22.750436139932052	21.715942827411013
8	29.10536262155607	30.560592497379513	22.738923191100298	17.595121689964117
9	28.213250089899848	16.6180824936305	20.47574234321084	34.69292507325881
10	19.76036908669406	31.184918248521438	29.24155132707477	19.813161337709733
11	25.579758379813466	27.128331076273327	16.92029900306807	30.371611540845134
12	26.256111276883882	33.51083771355996	26.00056617776452	14.232484831791648
13	31.585833314205708	19.84147022593553	22.469606200411626	26.103090259447136
14	23.210993029892656	29.201000757454036	25.51625465757722	22.071751555076087
15	37.673009387839414	17.677753039379958	23.33876557945234	21.310471993328285
16	16.14371733957659	26.783268681953466	36.15886642030283	20.914147558167116
17	26.992348890589135	30.2938026013772	26.530221882172917	16.18362662586075
18	25.83147795349691	20.645595672565626	33.037237664593235	20.48568870934423
19	22.498680193724606	31.32110695404014	33.18872847185561	12.991484380379644
20	24.728961522865163	23.158200778876978	29.36626345628572	22.746574241972134
21	17.210273831110705	28.354794531028837	34.192546346240654	20.242385291619804
22	22.070986449988904	27.70675052218422	22.64328505520233	27.578977972624543
23	15.696130863574112	26.732006641112154	32.23540753322469	25.336454962089043
24	21.128376982578555	35.060175515107	25.47570408795648	18.335743414357964
25	22.22877087702647	22.358128071276578	23.596589256464032	31.81651179523292
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1241.0
1	1241.0
2	1026.5
3	812.0
4	812.0
5	812.0
6	1072.5
7	1333.0
8	1333.0
9	1333.0
10	1189.0
11	1045.0
12	1045.0
13	1045.0
14	776.5
15	508.0
16	508.0
17	508.0
18	762.0
19	1016.0
20	1016.0
21	1016.0
22	1591.5
23	2167.0
24	2167.0
25	2167.0
26	2749.0
27	3331.0
28	3331.0
29	3331.0
30	3791.5
31	4252.0
32	4252.0
33	4252.0
34	4302.5
35	4353.0
36	4353.0
37	4353.0
38	4051.0
39	3749.0
40	3749.0
41	3749.0
42	5747.5
43	7746.0
44	7746.0
45	7746.0
46	28509.5
47	49273.0
48	49273.0
49	49273.0
50	40833.5
51	32394.0
52	32394.0
53	32394.0
54	21515.0
55	10636.0
56	10636.0
57	10636.0
58	6828.0
59	3020.0
60	3020.0
61	3020.0
62	2521.0
63	2022.0
64	2022.0
65	2022.0
66	1527.0
67	1032.0
68	1032.0
69	1032.0
70	754.5
71	477.0
72	477.0
73	477.0
74	339.0
75	201.0
76	201.0
77	201.0
78	136.0
79	71.0
80	71.0
81	71.0
82	42.0
83	13.0
84	13.0
85	13.0
86	9.5
87	6.0
88	6.0
89	6.0
90	4.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01300678648212332
2	0.009946366133388422
3	0.0
4	0.0
5	0.41239164199202755
6	0.03978546453355369
7	0.006885945784653522
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	7.651050871837247E-4
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.042080779795104856
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	130701.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.188039877277145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.46210446431517	19.81698686314565
2	8.859339426535124	4.108614318176602
3	2.1579173128320193	1.5011361810544679
4	0.9040815653149438	0.8385551755533623
5	0.42894380836110474	0.49731830666942106
6	0.31675850463589267	0.4407005302178254
7	0.21777147193717622	0.3534785502788808
8	0.15507968456132248	0.28767951278108045
9	0.14518098129145082	0.30298161452475497
>10	0.9469759461510543	4.132332575879297
>50	0.10888573596858811	1.7306677072095853
>100	0.16497838783119412	7.9157772320028155
>500	0.036295245322862706	6.5156349224565995
>1k	0.08908832942884481	38.72426377762986
>5k	0.006599135513247765	12.833872732419799
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8583	6.56689696329791	No Hit
CCCATGTACTCTGCGTTGATACCAC	8191	6.266975769121889	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4605	3.5233089264810524	No Hit
CATGTACTCTGCGTTGATACCACTG	4083	3.1239240709711478	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3062	2.342751776956565	No Hit
GCGTTGATACCACTGCTTCCCATGT	2994	2.2907246310280716	No Hit
GTATCAACGCAGAGTACATGGGAAG	2659	2.034414426821524	No Hit
TATCAACGCAGAGTACATGGGAAGC	2608	1.995394067375154	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2600	1.9892732266776842	No Hit
GGTATCAACGCAGAGTACATGGGAA	2503	1.9150580332208629	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2086	1.5960092118652496	No Hit
GTGGTATCAACGCAGAGTACATGGG	1785	1.3657125806229484	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1567	1.1989196716168964	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1561	1.1943290410937941	No Hit
GATACCACTGCTTCCCATGTACTCT	1455	1.1132279018523195	No Hit
ATACCACTGCTTCCCATGTACTCTG	1415	1.0826236983649704	No Hit
GTACTCTGCGTTGATACCACTGCTT	1397	1.0688518067956634	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1390	1.0634960711853774	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1360	1.0405429185698656	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1310	1.0022876642106793	No Hit
CAGTGGTATCAACGCAGAGTACATG	1298	0.9931064031644745	No Hit
ACATGGGAAGCAGTGGTATCAACGC	1174	0.8982333723536928	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	1137	0.8699244841278949	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1136	0.8691593790407113	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1115	0.853092172209853	No Hit
TACCACTGCTTCCCATGTACTCTGC	1102	0.8431458060764646	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1101	0.8423807009892809	No Hit
CCATGTACTCTGCGTTGATACCACT	1072	0.8201926534609528	No Hit
GTTGATACCACTGCTTCCCATGTAC	1038	0.7941790804967063	No Hit
CCACTGCTTCCCATGTACTCTGCGT	916	0.7008362598602917	No Hit
CTTCCCATGTACTCTGCGTTGATAC	916	0.7008362598602917	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	913	0.6985409445987406	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	905	0.6924201039012708	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	885	0.6771180021575963	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	803	0.614379385008531	No Hit
TGATACCACTGCTTCCCATGTACTC	712	0.544754822074812	No Hit
TTCCCATGTACTCTGCGTTGATACC	678	0.5187412491105653	No Hit
ATCAACGCAGAGTACATGGGAAGCA	648	0.49578809649505357	No Hit
GTACATGGTAAGCAGTGGTATCAAC	575	0.4399354251306417	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	565	0.43228437425880445	No Hit
ACCATGTACTCTGCGTTGATACCAC	465	0.355773865540432	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	443	0.33894155362239003	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	396	0.30298161452475497	No Hit
CTCTGCGTTGATACCACTGCTTCCC	362	0.27696804156050836	No Hit
CTGCGTTGATACCACTGCTTCCCAT	357	0.27314251612458973	No Hit
TCAACGCAGAGTACATGGGAAGCAG	335	0.25631020420654776	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	301	0.2302966312423011	No Hit
AAAAAGTACTCTGCGTTGATACCAC	295	0.22570600071919877	No Hit
GTGGTATCAACGCAGAGTACTTTTT	286	0.2188200549345453	No Hit
AAAGTACTCTGCGTTGATACCACTG	274	0.20963879388834059	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	274	0.20963879388834059	No Hit
TTGATACCACTGCTTCCCATGTACT	273	0.20887368880115684	No Hit
AACGCAGAGTACATGGGAAGCAGTG	271	0.2073434786267894	No Hit
TCCCATGTACTCTGCGTTGATACCA	267	0.20428305827805449	No Hit
TCCATGTACTCTGCGTTGATACCAC	255	0.1951017972318498	No Hit
ATGTACTCTGCGTTGATACCACTGC	243	0.18592053618564508	No Hit
GAGTACATGGTAAGCAGTGGTATCA	243	0.18592053618564508	No Hit
CAACGCAGAGTACATGGGAAGCAGT	240	0.18362522092409392	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	235	0.1797996954881753	No Hit
AGTGGTATCAACGCAGAGTACATGG	232	0.17750438022662413	No Hit
CCCCATGTACTCTGCGTTGATACCA	229	0.17520906496507294	No Hit
GTACATGGAAAGCAGTGGTATCAAC	210	0.1606720683085822	No Hit
GCTTACCATGTACTCTGCGTTGATA	203	0.15531633269829612	No Hit
GTACATGGGTAAGCAGTGGTATCAA	198	0.15149080726237749	No Hit
CGCAGAGTACATGGGAAGCAGTGGT	188	0.14383975639054022	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	187	0.1430746513033565	No Hit
CGTTGATACCACTGCTTCCCATGTA	163	0.12471212921094713	No Hit
ACTCTGCGTTGATACCACTGCTTAC	163	0.12471212921094713	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	160	0.12241681394939595	No Hit
ATGGTAAGCAGTGGTATCAACGCAG	152	0.11629597325192614	No Hit
GAGTACATGGAAAGCAGTGGTATCA	144	0.11017513255445635	No Hit
GCGTTGATACCACTGCTTACCATGT	142	0.10864492238008891	No Hit
TGTACTCTGCGTTGATACCACTGCT	139	0.10634960711853773	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	138	0.105584502031354	No Hit
GTATCAACGCAGAGTACATGGTAAG	138	0.105584502031354	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	134	0.1025240816826191	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	133	0.1017589765954354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.651050871837247E-4	0.0
6	0.0	0.0	0.0	7.651050871837247E-4	0.0
7	0.0	0.0	0.0	7.651050871837247E-4	0.0
8	0.0	0.0	0.0	7.651050871837247E-4	0.0
9	0.0	0.0	0.0	7.651050871837247E-4	0.0
10	0.0	0.0	0.0	0.0015302101743674495	0.0
11	0.0	0.0	0.0	0.0015302101743674495	0.0
12	0.0	0.0	0.0	0.0015302101743674495	0.0
13	0.0	0.0	0.0	0.0015302101743674495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTTC	40	0.00511154	14.305364	1
GAACAAA	40	0.00511154	14.305364	1
GAAAAAA	105	4.2533712E-4	9.08277	1
AACAAAA	105	4.302778E-4	9.072307	2
CATGGTA	160	6.260161E-7	8.930552	4
ACATGGT	150	2.5261852E-6	8.890861	3
ATGGTAA	160	6.0346247E-6	8.335181	5
TGGTAAG	175	2.0943839E-5	7.5944886	6
CCATGTA	2690	0.0	7.330356	2
ACAAAAA	130	0.0035550424	7.327632	3
TAAGCAG	185	4.394535E-5	7.1812277	9
TGCGTTG	2910	0.0	6.978518	12
CTGCGTT	2930	0.0	6.930883	11
CCCATGT	2620	0.0	6.916079	1
TACTCTG	2955	0.0	6.8722463	7
CAGTGGT	2585	0.0	6.8280206	13
GTAAGCA	195	8.780352E-5	6.812959	8
AGTGGTA	2610	0.0	6.7989764	14
CTCTGCG	3005	0.0	6.757899	9
TCTGCGT	3020	0.0	6.724334	10
>>END_MODULE