FastQCFastQC Report
Wed 25 May 2016
SRR1294804_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294804_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765470
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC38490.1391806817647633No Hit
CCCATGTACTCTGCGTTGATACCAC36800.13306960480496988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG602.565434E-514.2525025
ATACCGT750.00266078210.1277686
GGCGCAA856.637522E-410.05202515
TAGGACT3300.09.7899524
GCTATAC1856.475602E-109.7581163
TCTACAC2600.09.5013243
CCGTACC900.00111544149.498749
TCCGTAC800.0045222679.498748
GTATTAG3600.09.2377331
GTATAGA3000.09.1849451
GTCCTAT2501.8189894E-129.1216011
GGACCGT850.0074713948.9362676
CGATTTC850.00748027958.93497216
CTAGGAC3300.08.9254863
GTATTAA5750.08.92330551
TATACTG5150.08.8559245
CGGGTTC1201.7750345E-48.7035518
CTAAGAC2106.368282E-98.5964363
TTGGCGT1000.0029003178.5518114
TACACCG1000.00290126918.55155
Produced by FastQC (version 0.11.5)