FastQCFastQC Report
Wed 25 May 2016
SRR1294804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294804_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765470
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69940.2529045695668367No Hit
GGTATCAACGCAGAGTACTTTTTTT41810.15118587437216818No Hit
GTACATGGGAAGCAGTGGTATCAAC38570.13946996351433932No Hit
CCCATGTACTCTGCGTTGATACCAC37840.13683026754945812No Hit
TATCAACGCAGAGTACTTTTTTTTT32630.11799079360831975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC400.005320627714.2337387
GTCTAGA2150.011.0856261
TGAACGG900.00112708939.48795810
TCGTGCA900.00112708939.4879589
CTAAGGC1901.0017175E-89.02907853
CGGGTTC1901.081753E-88.98859218
GCGTAGG850.0072252398.9728361
ACGAAAC850.00751336068.93016615
TCTATAC2701.8189894E-128.8247173
CTGTCGC2401.05501385E-108.6972959
CTAGACA2106.024493E-98.6230094
GGTATCA26550.08.6179771
TAACACG1000.00282224768.5776244
ACATCGC1000.0029269548.5431796
CGAATAG1000.00293847528.53947215
GCGAGGC1000.00293847528.53947211
CTTACCC2803.6379788E-128.5095483
TAGGACC1803.8426333E-78.4717274
GTCTTAG2951.8189894E-128.4025281
TACTCCG1252.6503575E-48.3870115
Produced by FastQC (version 0.11.5)