FastQCFastQC Report
Wed 25 May 2016
SRR1294803_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294803_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726056
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC81970.21999132594893905No Hit
CCCATGTACTCTGCGTTGATACCAC75410.20238557874599847No Hit
GATCAGCACCATGAAGCTTCTCACG47150.1265413080211355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC759.668402E-713.9352174
TCGTCTA1403.6379788E-1212.88662814
TCGTCCG1551.8189894E-1212.2557669
TCTCACG6950.011.80413419
TTAGACT2350.011.3206784
CGCACGA951.3657525E-510.99690212
TAGGACA3900.010.2321524
ACCGAGT1303.856021E-710.2288518
GACGCGT750.002656732510.1297996
ACCGTCT1901.0004442E-109.9981258
GTCGTAC1054.1053223E-59.9530571
GTCACGC1054.1168623E-59.9505158
GTCCCGC2600.09.86605551
AACCGTG1451.623921E-79.8256117
GTCTAGA3000.09.8173331
ATCGTCC2350.09.7003088
TAGGACC2550.09.6875844
GTATTAG3350.09.64244651
TAAGACG900.00111290469.5012854
GTCTAAG2401.8189894E-129.5006461
Produced by FastQC (version 0.11.5)