FastQCFastQC Report
Wed 25 May 2016
SRR1294803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726056
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC79700.21389909330401907No Hit
GTACATGGGAAGCAGTGGTATCAAC78150.20973919876673888No Hit
GTATCAACGCAGAGTACTTTTTTTT50680.13601513235442517No Hit
GATCAGCACCATGAAGCTTCTCACG48230.12943981518259523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT801.3021963E-714.2390776
CGTCCGC1500.013.28534610
TACGACC603.9971198E-412.7028264
ACCGCGT604.1326604E-412.6527118
GCTCGTT2700.012.65271111
CTCGTTC2100.012.20082912
TCGTCCG1353.783498E-1011.9497829
TGCTCGT2350.011.71048810
CCGTCCG658.1026164E-411.6794259
GGGTACG907.2089933E-611.6480241
ACCGTCT1701.2732926E-1111.1641578
TAAACTG4200.010.6613015
TACCGTG1252.2760833E-710.6287057
TCTCACG8950.010.39481919
GCGGGAC1658.8584784E-1010.3932213
TAGCCCG1201.4705929E-610.3210465
CGACCAT1201.5404548E-610.2837776
TTAGACA3000.010.162264
TCGTCTA1804.0199666E-1010.01672914
GTGTTAG4150.09.8746661
Produced by FastQC (version 0.11.5)