FastQCFastQC Report
Wed 25 May 2016
SRR1294802_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294802_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575061
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC178053.0961932734092557No Hit
CCCATGTACTCTGCGTTGATACCAC169442.9464700266580413No Hit
GAGTACATGGGAAGCAGTGGTATCA89361.5539221056548784No Hit
CATGTACTCTGCGTTGATACCACTG87221.5167086622114871No Hit
GCGTTGATACCACTGCTTCCCATGT55120.9585070105606188No Hit
ACGCAGAGTACATGGGAAGCAGTGG53790.9353790293551467No Hit
ACTCTGCGTTGATACCACTGCTTCC45910.7983500880776127No Hit
TATCAACGCAGAGTACATGGGAAGC40870.7107072119305604No Hit
GTATCAACGCAGAGTACATGGGAAG40240.6997518524121789No Hit
GGTATCAACGCAGAGTACATGGGAA40140.6980129064568802No Hit
GTGGTATCAACGCAGAGTACATGGG32990.5736782706530265No Hit
GCTTCCCATGTACTCTGCGTTGATA26900.46777646197533823No Hit
GTACTCTGCGTTGATACCACTGCTT26890.4676025673798084No Hit
CAGTGGTATCAACGCAGAGTACATG25390.44151837805032856No Hit
ATACCACTGCTTCCCATGTACTCTG25020.4350842780157235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA24040.4180426076537967No Hit
GTATCAACGCAGAGTACTTTTTTTT23900.41560808331637855No Hit
GATACCACTGCTTCCCATGTACTCT23240.4041310400114075No Hit
GTTGATACCACTGCTTCCCATGTAC23120.4020443048650491No Hit
GCAGAGTACATGGGAAGCAGTGGTA22040.3832636885478236No Hit
CTGCTTCCCATGTACTCTGCGTTGA21980.3822203209746444No Hit
GGGAAGCAGTGGTATCAACGCAGAG21770.37856853446851724No Hit
CATGGGAAGCAGTGGTATCAACGCA20980.3648308614216579No Hit
GCAGTGGTATCAACGCAGAGTACAT20770.3611790749155307No Hit
TACCACTGCTTCCCATGTACTCTGC18460.32100942334813176No Hit
CCACTGCTTCCCATGTACTCTGCGT17940.3119669043805787No Hit
AAGCAGTGGTATCAACGCAGAGTAC17460.3036199637951452No Hit
GGTATCAACGCAGAGTACTTTTTTT16640.28936060696169624No Hit
CTTCCCATGTACTCTGCGTTGATAC15980.2778835636567251No Hit
ACATGGGAAGCAGTGGTATCAACGC15030.2613635770813879No Hit
TATCAACGCAGAGTACTTTTTTTTT14010.2436263283373416No Hit
ATGGGAAGCAGTGGTATCAACGCAG13590.23632275532508726No Hit
TGATACCACTGCTTCCCATGTACTC11430.1987615226906363No Hit
CCATGTACTCTGCGTTGATACCACT11100.19302300103815073No Hit
GTACATGGTAAGCAGTGGTATCAAC10090.1754596468896343No Hit
TTCCCATGTACTCTGCGTTGATACC9650.16780828468632025No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7940.13807230885071323No Hit
ACCATGTACTCTGCGTTGATACCAC7900.13737673046859378No Hit
ATCAACGCAGAGTACATGGGAAGCA7210.12537800337703303No Hit
AAACAAAAAAAAAAAAAAAAAAAAA7040.12242179525302534No Hit
GGAAGCAGTGGTATCAACGCAGAGT6960.12103063848878642No Hit
CTGCGTTGATACCACTGCTTCCCAT5820.10120665459838174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC508.690502E-515.2016743
ACCTAAG456.747496E-414.7794051
GTACAAA602.5600304E-514.251571
GGGGTTA551.9636266E-413.8124786
TAACCCT500.001494328113.3037854
GAACAAA2500.013.3014661
ACCAATT604.1053217E-412.65923215
CAGTACT550.003057864512.094354
AACCCTA550.003059862612.0932965
CTTGCAA657.725734E-411.74273719
TGAAAGT658.037683E-411.68748213
TTGGCTC855.3219872E-511.17380410
CTGTGAC855.3219872E-511.1738049
TAAGAAC600.00585558511.0864874
TGGTTCA600.005859387611.0855215
AGGTCGG600.00587843211.0806910
GTCAGGC600.005889884711.07779417
AAGTGGT600.005889884711.07779416
AGCACTG700.001486469510.8592845
TGCCCAC700.001494057210.85266111
Produced by FastQC (version 0.11.5)