FastQCFastQC Report
Wed 25 May 2016
SRR1294802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575061
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC179663.1241903032895637No Hit
GTACATGGGAAGCAGTGGTATCAAC170352.962294434851259No Hit
GAGTACATGGGAAGCAGTGGTATCA91031.582962503108366No Hit
CATGTACTCTGCGTTGATACCACTG89711.5600084164984236No Hit
GTATCAACGCAGAGTACTTTTTTTT58381.0151966487033548No Hit
GCGTTGATACCACTGCTTCCCATGT57511.0000678188922565No Hit
ACGCAGAGTACATGGGAAGCAGTGG55700.9685928971013509No Hit
ACTCTGCGTTGATACCACTGCTTCC48170.8376502666673622No Hit
TATCAACGCAGAGTACATGGGAAGC42270.7350524553047416No Hit
GGTATCAACGCAGAGTACTTTTTTT42210.7340090877315625No Hit
GGTATCAACGCAGAGTACATGGGAA39220.6820146036681326No Hit
GTATCAACGCAGAGTACATGGGAAG38730.6734937684871692No Hit
TATCAACGCAGAGTACTTTTTTTTT36710.6383670601901363No Hit
GTGGTATCAACGCAGAGTACATGGG32550.5660269084497123No Hit
GCTTCCCATGTACTCTGCGTTGATA27400.4764711917518315No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27330.4752539295831225No Hit
CAGTGGTATCAACGCAGAGTACATG26210.45577773488377754No Hit
GTACTCTGCGTTGATACCACTGCTT25400.4416922726458584No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25350.4408227996682091No Hit
ATACCACTGCTTCCCATGTACTCTG24790.43108470231853663No Hit
CATGGGAAGCAGTGGTATCAACGCA23360.40621777515776586No Hit
CTGCTTCCCATGTACTCTGCGTTGA23130.40221819946057896No Hit
GATACCACTGCTTCCCATGTACTCT23020.40030535890975044No Hit
GCAGAGTACATGGGAAGCAGTGGTA21910.3810030588059354No Hit
GGGAAGCAGTGGTATCAACGCAGAG21510.3740472749847408No Hit
GTTGATACCACTGCTTCCCATGTAC21480.37352559119815115No Hit
GCAGTGGTATCAACGCAGAGTACAT21400.37213443443391225No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19810.3444851937446636No Hit
CCACTGCTTCCCATGTACTCTGCGT19600.34083340723853645No Hit
TACCACTGCTTCCCATGTACTCTGC18880.32831299636038613No Hit
AAGCAGTGGTATCAACGCAGAGTAC18490.32153110713472133No Hit
CTTCCCATGTACTCTGCGTTGATAC16540.2876216610063976No Hit
ACATGGGAAGCAGTGGTATCAACGC16100.2799702988030835No Hit
ATGGGAAGCAGTGGTATCAACGCAG14020.24380022293287149No Hit
TGATACCACTGCTTCCCATGTACTC12240.21284698492855542No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11380.19789204971298696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11330.19702257673533766No Hit
CCATGTACTCTGCGTTGATACCACT11290.19632699835321815No Hit
TTCCCATGTACTCTGCGTTGATACC10340.17980701177788097No Hit
GTACATGGTAAGCAGTGGTATCAAC9560.16624323332655144No Hit
ACCATGTACTCTGCGTTGATACCAC8060.14015904399707163No Hit
ATCAACGCAGAGTACATGGGAAGCA7800.1356377845132951No Hit
GGAAGCAGTGGTATCAACGCAGAGT7170.1246824249949136No Hit
GTGGTATCAACGCAGAGTACTTTTT6960.12103063848878642No Hit
CTGCGTTGATACCACTGCTTCCCAT6020.10468454650897904No Hit
ATCAACGCAGAGTACTTTTTTTTTT5830.1013805491939116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCA453.4254743E-516.9496293
AGTACTG350.002116574216.3442865
TGCATAA704.4914304E-714.90800814
ACCGACC456.8241113E-414.75742158
TCTAATA653.2372081E-614.669232
CCATAGT655.25622E-513.2023072
AATACTG655.260534E-513.2011545
AACCGAC655.5116594E-513.1357287
CTCTAAT751.4200004E-512.7133331
GATGCTA853.9921706E-612.27718314
CATAATG701.1026939E-412.19746116
GTCCTAA550.002981120312.1354531
GTACTGG550.002981120312.1354531
TTTAGAG550.002983072812.1343943
CCTATAC1209.438736E-911.9177083
TAATACT1052.5848385E-711.8042064
GTAGTGG907.139095E-611.6538881
AATGAAT907.487053E-611.604197519
CTATACC1251.7269485E-811.4414
GAAACCG752.0015813E-411.4415
Produced by FastQC (version 0.11.5)