FastQCFastQC Report
Wed 25 May 2016
SRR1294801_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294801_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658233
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC23720.14304383039054222No Hit
GTATCAACGCAGAGTACTTTTTTTT21800.13146524040952026No Hit
GAATGGAATGGAATGGAATGGAATG18540.11180575950424337No Hit
GTACATGGGAAGCAGTGGTATCAAC16910.10197601905160493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGT602.5649924E-514.252044
AGTACCG400.005275338414.251615
GTACGAC400.00527957214.2498863
GGTCGGC500.001502785413.29627711
TTGAGCG603.9649065E-412.71422519
ACGGGTC604.0883766E-412.66809755
CGATATT550.00307990512.08496914
TCGGTCT802.8766268E-511.8709576
GTCTCGT752.0776194E-411.3978419
CTCGGTC1201.2732016E-711.0845855
ACGGGGA600.005884217611.0812358
GCTCGTC600.0058881911.0802312
CGTCTAT600.005896141811.07822115
CCTCGGT1053.4601035E-610.8586994
TGTCGCC1158.834013E-710.73742110
TGCACGG803.770175E-410.6877382
GGTATCA15750.010.4957891
TATTCCG1106.0366656E-610.3648085
CTAGAAC2200.010.3635543
TCGTGGG1659.331416E-1010.36073512
Produced by FastQC (version 0.11.5)