FastQCFastQC Report
Wed 25 May 2016
SRR1294801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658233
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76110.4589825434664489No Hit
GGTATCAACGCAGAGTACTTTTTTT50080.30200822200498967No Hit
TATCAACGCAGAGTACTTTTTTTTT44120.26606634893890063No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24810.14961709241101823No Hit
ATTCCATTCCATTCCATTCCATTCC21550.12995761150574134No Hit
GAATGGAATGGAATGGAATGGAATG19110.11524315340485927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAC453.4465796E-516.9411353
GACCGGA350.002125956616.3360962
TGGACCG456.606108E-414.8234945
GGTCGGC500.001513265413.28402211
ACCGAGT604.133989E-412.6514498
GCTATAC853.8098697E-612.332153
ACCGTCT701.1032958E-412.1996128
GCTCGTT701.1032958E-412.19961211
TGGTCGG658.1052375E-411.67826110
GGTATCA17900.011.2350081
TATTCCG803.6648277E-410.7205635
TCAGTAC1252.1464257E-710.6729163
GCCCTAG909.20464E-510.5901351
CCGGACT909.2216185E-510.588214
TTGCGTG856.6648534E-410.04704216
CCGTCTG856.6667015E-410.046749
AGCACCG951.5935114E-410.0309365
AGTACCT2002.3646862E-1110.005865
TAGACAG1909.931682E-109.5293895
GCAGTAC1502.5309055E-79.5293893
Produced by FastQC (version 0.11.5)