FastQCFastQC Report
Wed 25 May 2016
SRR1294800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103581
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC114850.37005639614368047No Hit
CCCATGTACTCTGCGTTGATACCAC104690.33732001839165787No Hit
GAGTACATGGGAAGCAGTGGTATCA52740.16993273254347158No Hit
GCGTTGATACCACTGCTTCCCATGT39260.12649903450240224No Hit
CATGTACTCTGCGTTGATACCACTG38630.12446912131502286No Hit
GTATCAACGCAGAGTACATGGGAAG37990.12240698728339941No Hit
ATTCCATTCCATTCCATTCCATTCC33300.10729541133290867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGG456.779805E-414.7735211
CGGAGCG551.9602932E-413.8188863
AGCGTAG550.003067714412.0919161
AGCGCGG658.038078E-411.6904510
CGTAGCG752.0740999E-411.4005813
GTAGCGT855.316813E-511.1784854
GCGTAGC855.3229403E-511.1772212
CGCCTAT600.00587424511.0842571
CTCGCGG600.005890508611.0801411
GGACGGT600.005896174311.078716
TCACCGG909.574047E-510.54978816
TATTCCG1502.4414476E-810.1349955
TAGCGTC856.586936E-410.0604745
TACATCG856.586936E-410.0604745
GTCCTAA2650.09.6800521
GTGTAGA4950.09.596761
AACACCG900.00111249969.5015595
GCACCGT1502.6668022E-79.4960376
GTCACCG900.00111975299.4948115
CTAGCAC3650.09.3703413
Produced by FastQC (version 0.11.5)