FastQCFastQC Report
Wed 25 May 2016
SRR1294800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103581
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC116200.3744062101166362No Hit
CCCATGTACTCTGCGTTGATACCAC109540.3529471278500545No Hit
GTATCAACGCAGAGTACTTTTTTTT87720.2826412457093918No Hit
GGTATCAACGCAGAGTACTTTTTTT56280.18133891140588887No Hit
GAGTACATGGGAAGCAGTGGTATCA54400.17528139268799492No Hit
TATCAACGCAGAGTACTTTTTTTTT51210.1650029433741217No Hit
GCGTTGATACCACTGCTTCCCATGT39890.12852894768978157No Hit
CATGTACTCTGCGTTGATACCACTG38880.1252746424211258No Hit
GTATCAACGCAGAGTACATGGGAAG38370.12363137936467583No Hit
ATTCCATTCCATTCCATTCCATTCC31810.10249450554053528No Hit
ACGCAGAGTACATGGGAAGCAGTGG31050.10004572137798241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA456.82911E-414.75940812
ACCGTTC707.362096E-613.5545598
ACCGGGT550.003098356312.0758798
TATACCG657.8107195E-411.73055
CGATGGG803.8165663E-410.67421413
ACGGGTC909.211483E-510.59003455
AAACCCG856.4020185E-410.091685
TGTACCG856.4020185E-410.091685
TCTACAC2700.09.8840323
GTCCTAA2700.09.5327281
GTCTAGG3600.09.5327281
TACACCG1002.6679822E-49.5310325
TAGTACG800.00439766389.5310324
ACACCGT1201.7192484E-59.4917086
CCGTGTA800.00456417459.4881919
CGATTTC1201.7268056E-59.48819116
GTATAGG4150.09.4178761
GTCTAAT3350.09.3904491
GTCTTAG2950.09.3711571
CTAGGAC3100.09.2235793
Produced by FastQC (version 0.11.5)