FastQCFastQC Report
Wed 25 May 2016
SRR1294799_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294799_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2308730
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC77160.3342097170305753No Hit
CCCATGTACTCTGCGTTGATACCAC70210.3041065867381634No Hit
GAGTACATGGGAAGCAGTGGTATCA33770.14627089352154649No Hit
CATGTACTCTGCGTTGATACCACTG26270.11378550111966318No Hit
GCGTTGATACCACTGCTTCCCATGT25860.11200963300169357No Hit
GTATCAACGCAGAGTACTTTTTTTT24830.10754830577850161No Hit
GTATCAACGCAGAGTACATGGGAAG23370.10122448272426833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG400.00528629314.2474939
GTCGTAC500.001497748913.3025681
GTCTTAG2350.012.938671
TCGTGGG2000.012.34702312
ACCGTCT802.8729351E-511.87291058
AACCCGT600.005901003711.0772946
GTCTCGT951.363529E-510.9980659
TAGGACC1605.4205884E-1010.6904944
GTACTAA2053.6379788E-1210.1970921
GTCTAGC1409.6664735E-810.1805371
GTCTAAG1701.5552359E-910.0607661
CGTAGTA1054.0978382E-59.9543032
GTAGCAC1651.0193617E-89.7897693
TCGTAGG800.00450965259.5018352
GCTACAC2401.8189894E-129.5018353
TGAGCGA800.00452421569.49812210
TGTCGTG800.0045258369.49770911
AGACCGT1201.7119746E-59.4948236
GCGTCGC800.00454288359.493381514
TCTACAC2451.8189894E-129.307923
Produced by FastQC (version 0.11.5)