FastQCFastQC Report
Wed 25 May 2016
SRR1294799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294799_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2308730
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC79950.34629428300407583No Hit
GTATCAACGCAGAGTACTTTTTTTT79410.3439553347511402No Hit
CCCATGTACTCTGCGTTGATACCAC73640.31896323952995803No Hit
GGTATCAACGCAGAGTACTTTTTTT52560.22765762995239808No Hit
TATCAACGCAGAGTACTTTTTTTTT47440.20548093540604576No Hit
GAGTACATGGGAAGCAGTGGTATCA37690.16324992528359747No Hit
CATGTACTCTGCGTTGATACCACTG27180.11772706206442501No Hit
GCGTTGATACCACTGCTTCCCATGT26180.11339567641084058No Hit
GTATCAACGCAGAGTACATGGGAAG24560.10637883165203381No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23400.10135442429387587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGC508.777974E-515.1875536
AATTTCG604.1398616E-412.64971515
ATCGTCC752.1010307E-411.3844988
ACGCTAA600.005913064311.0742586
ATTTCGT951.3812229E-510.98527916
CATCCCG803.8198964E-410.67296610
GTTTAAC1553.448804E-910.4579591
TCTACAC3050.010.3149763
AGCACCG1409.206815E-810.2145055
TGCTACG750.002570693810.1697712
TCGTTTG750.002677985710.11977213
GTCCTAG2450.010.1189861
TAACACC1909.276846E-1110.0353034
CGAGTGG1356.566679E-79.83845410
CTACACT3300.09.82243254
GTCTAAC1304.0449904E-69.5351981
ACACCGA1002.7789045E-49.4922216
AGAGCGC800.00456783369.4870818
TTACACT3350.09.3912464
TAGGACT3150.09.3822124
Produced by FastQC (version 0.11.5)