FastQCFastQC Report
Wed 25 May 2016
SRR1294798_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294798_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308468
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC121553.940441147866229No Hit
CCCATGTACTCTGCGTTGATACCAC116003.760519729761272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA89212.8920341818276127No Hit
GAGTACATGGGAAGCAGTGGTATCA66072.1418753322873036No Hit
CATGTACTCTGCGTTGATACCACTG53601.7376194613379672No Hit
GCGTTGATACCACTGCTTCCCATGT44011.4267282181620136No Hit
ACGCAGAGTACATGGGAAGCAGTGG43361.4056563403659375No Hit
GGTATCAACGCAGAGTACATGGGAA40541.3142368090044998No Hit
TATCAACGCAGAGTACATGGGAAGC37581.2182787193485223No Hit
GTATCAACGCAGAGTACATGGGAAG37531.216657805671901No Hit
ACTCTGCGTTGATACCACTGCTTCC37301.2092016027594434No Hit
GTGGTATCAACGCAGAGTACATGGG32581.0561873516863984No Hit
GTATCAACGCAGAGTACTTTTTTTT29550.9579599828831515No Hit
GGTATCAACGCAGAGTACTTTTTTT25020.8111052037812674No Hit
GTACTCTGCGTTGATACCACTGCTT24760.8026764526628369No Hit
GCTTCCCATGTACTCTGCGTTGATA24000.7780385647781942No Hit
GATACCACTGCTTCCCATGTACTCT21970.712229469507372No Hit
ATACCACTGCTTCCCATGTACTCTG20590.6674922520326257No Hit
CAGTGGTATCAACGCAGAGTACATG19420.6295628719996887No Hit
CTGCTTCCCATGTACTCTGCGTTGA18720.6068700805269914No Hit
GGGAAGCAGTGGTATCAACGCAGAG17970.5825563753776729No Hit
CATGGGAAGCAGTGGTATCAACGCA17950.5819080099070244No Hit
TATCAACGCAGAGTACTTTTTTTTT17530.568292335023406No Hit
GCAGAGTACATGGGAAGCAGTGGTA16470.5339289650790358No Hit
GCAGTGGTATCAACGCAGAGTACAT16020.5193407419894446No Hit
ACATGGGAAGCAGTGGTATCAACGC15970.5177198283128234No Hit
GTTGATACCACTGCTTCCCATGTAC15850.5138296354889325No Hit
TACCACTGCTTCCCATGTACTCTGC15380.4985930469286927No Hit
CCACTGCTTCCCATGTACTCTGCGT14570.47233424536742874No Hit
CTTCCCATGTACTCTGCGTTGATAC14550.47168587989678024No Hit
CCATGTACTCTGCGTTGATACCACT12210.3958271198309063No Hit
AAGCAGTGGTATCAACGCAGAGTAC12210.3958271198309063No Hit
ATGGGAAGCAGTGGTATCAACGCAG11980.3883709169184486No Hit
TGATACCACTGCTTCCCATGTACTC10690.3465513440616207No Hit
TTCCCATGTACTCTGCGTTGATACC10660.3455787958556479No Hit
CCCTATGTATTCAGATGTGGGTAAC10560.3423369685024054No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10130.3283971108834628No Hit
GTACTTAGATGTTTCAGTTCCCTAG9790.3173748978824384No Hit
GGTATCAACGCAGAGTACATGGGGG9370.30375922299881997No Hit
AAACAAAAAAAAAAAAAAAAAAAAA8930.2894951826445531No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA8860.28722590349728333No Hit
ATCAACGCAGAGTACATGGGAAGCA8690.28171479699677116No Hit
ACTTAGATGTTTCAGTTCCCTAGGT7510.24346123422850993No Hit
GGGTAACAGAGGTTCGCTCTGCTGG6930.22465863557970356No Hit
GGAAGCAGTGGTATCAACGCAGAGT6820.22109262549113684No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6770.2194717118145156No Hit
GTACATGGTAAGCAGTGGTATCAAC6580.2133122398433549No Hit
CCACACACCCTATGTATTCAGATGT6420.20812531607816695No Hit
CCTCTAGGTACTTAGATGTTTCAGT5960.19321291025325155No Hit
CTGCGTTGATACCACTGCTTCCCAT5910.1915919965766303No Hit
CTCTGCGTTGATACCACTGCTTCCC5820.1886743519587121No Hit
GTGGATGCCTTGGCAGTCGAACCGA5690.18445997639949688No Hit
GTGGTATCAACGCAGAGTACTTTTT5550.1799214181049574No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5410.17538285981041793No Hit
ATTCAGATGTGGGTAACAGAGGTTC5400.1750586770750937No Hit
TGGGAAGCAGTGGTATCAACGCAGA5240.16987175330990573No Hit
AAAAAGTACTCTGCGTTGATACCAC5190.1682508396332845No Hit
CTGCTGGGTTTCCCCATTCGGACAT5150.1669541086919875No Hit
TCAACGCAGAGTACATGGGAAGCAG4870.15787699210290856No Hit
ACCATGTACTCTGCGTTGATACCAC4730.15333843380836912No Hit
GTATTCAGATGTGGGTAACAGAGGT4680.15171752013174786No Hit
AAAGTACTCTGCGTTGATACCACTG4310.13972275892475072No Hit
AACGCAGAGTACATGGGAAGCAGTG4170.13518420063021122No Hit
CACCCTATGTATTCAGATGTGGGTA4150.13453583515956274No Hit
AAAAACAAAAAAAAAAAAAAAAAAA4050.13129400780632028No Hit
CCCTAGGTTCCCTCCACACACCCTA3940.12772799771775353No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3930.12740381498242928No Hit
GGTATCAACGCAGAGTACATGGGGA3900.12643126677645655No Hit
AGTGGTATCAACGCAGAGTACATGG3860.12513453583515957No Hit
TCCATGTACTCTGCGTTGATACCAC3810.12351362215853832No Hit
ATGTACTCTGCGTTGATACCACTGC3630.11767833292270187No Hit
TTTCAGTTCCCTAGGTTCCCTCCAC3610.11702996745205337No Hit
GGTAAGCAGTGGTATCAACGCAGAG3550.11508487104010788No Hit
ACTGCTTCCCATGTACTCTGCGTTG3540.11476068830478364No Hit
TCCCATGTACTCTGCGTTGATACCA3500.11346395736348666No Hit
CCCCATGTACTCTGCGTTGATACCA3460.11216722642218965No Hit
TTGATACCACTGCTTCCCATGTACT3460.11216722642218965No Hit
TCCCTAGGTTCCCTCCACACACCCT3460.11216722642218965No Hit
TCCCCGGATCAATGTTTGCTTCCAA3420.11087049548089267No Hit
CCTCCACACACCCTATGTATTCAGA3410.11054631274556842No Hit
ACACAAAAAAAAAAAAAAAAAAAAA3390.10989794727491993No Hit
GCCTCGGGGAGTTGGAAGCAAACAT3350.10860121633362295No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA3350.10860121633362295No Hit
TAACAGAGGTTCGCTCTGCTGGGTT3100.10049664795051674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA855.638867E-1116.770581
GAACAAA3050.014.332891
AAGGGAG400.005276859714.2457413
GAGAACA655.407039E-513.1584561
AGAAGTT550.003054547612.0931835
GGAACCT1201.18567186E-711.13241219
CTCTAGG1053.4218629E-610.8609472
GAAAACA2200.010.7992371
TAACAAC803.740074E-410.6912451
TTTGCTT1054.1032894E-59.946177515
AATCAAC1803.8604412E-89.01195219
ACCTCTG850.0074446018.93560315
TCTAGGT1401.0717376E-58.8230873
TGTTTGC1201.756792E-48.7057313
ATGTTTG1201.756792E-48.7057312
AGGGAAC1356.789648E-58.4391817
GGGTAAG1252.732483E-48.3575017
AATGTTT1252.732483E-48.35750111
TTGCTTC1252.742081E-48.3547916
CTTCCAA1150.00105667878.2974519
Produced by FastQC (version 0.11.5)