FastQCFastQC Report
Wed 25 May 2016
SRR1294798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294798_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308468
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC126354.096048860821868No Hit
CCCATGTACTCTGCGTTGATACCAC122923.984854182605651No Hit
GTATCAACGCAGAGTACTTTTTTTT85732.779218589934774No Hit
GGTATCAACGCAGAGTACTTTTTTT73682.388578393869056No Hit
GAGTACATGGGAAGCAGTGGTATCA67762.1966622145571013No Hit
CATGTACTCTGCGTTGATACCACTG55311.7930547090784132No Hit
TATCAACGCAGAGTACTTTTTTTTT55261.7914337954017918No Hit
ACGCAGAGTACATGGGAAGCAGTGG48641.5768248246171401No Hit
GCGTTGATACCACTGCTTCCCATGT44081.4289974973092832No Hit
GGTATCAACGCAGAGTACATGGGAA41641.349896909890167No Hit
TATCAACGCAGAGTACATGGGAAGC38571.2503728101456228No Hit
ACTCTGCGTTGATACCACTGCTTCC38551.2497244446749745No Hit
GTATCAACGCAGAGTACATGGGAAG36691.1894264559046643No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35471.1498761621951061No Hit
GTGGTATCAACGCAGAGTACATGGG33761.0944409144546596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA31491.0208514335360555No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26280.8519522284321226No Hit
GCTTCCCATGTACTCTGCGTTGATA25740.8344463607246132No Hit
GTACTCTGCGTTGATACCACTGCTT23650.7666921690418456No Hit
GATACCACTGCTTCCCATGTACTCT23630.7660438035711971No Hit
CATGGGAAGCAGTGGTATCAACGCA20750.6726791757978137No Hit
ATACCACTGCTTCCCATGTACTCTG20620.6684648002385986No Hit
CAGTGGTATCAACGCAGAGTACATG20340.6593876836495195No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19710.638964171324092No Hit
GGGAAGCAGTGGTATCAACGCAGAG19370.6279419583230675No Hit
ACATGGGAAGCAGTGGTATCAACGC18380.5958478675259671No Hit
CTGCTTCCCATGTACTCTGCGTTGA17720.5744518069945667No Hit
GCAGAGTACATGGGAAGCAGTGGTA17640.5718583451119726No Hit
GCAGTGGTATCAACGCAGAGTACAT17210.5579184874930301No Hit
GTTGATACCACTGCTTCCCATGTAC15910.5157747319008779No Hit
TACCACTGCTTCCCATGTACTCTGC15590.5054008843705019No Hit
CTTCCCATGTACTCTGCGTTGATAC15340.4972963159873957No Hit
GTGGTATCAACGCAGAGTACTTTTT14790.4794662655445621No Hit
CCACTGCTTCCCATGTACTCTGCGT14580.47265842810275294No Hit
AAGCAGTGGTATCAACGCAGAGTAC13370.43343231712851904No Hit
CCATGTACTCTGCGTTGATACCACT13110.42500356601008854No Hit
ATGGGAAGCAGTGGTATCAACGCAG13070.42370683506879153No Hit
GCAGAGTACTTTTTTTTTTTTTTTT12890.4178715458329551No Hit
CCCTATGTATTCAGATGTGGGTAAC11310.366650673651724No Hit
TGATACCACTGCTTCCCATGTACTC10890.3530349987681056No Hit
TTCCCATGTACTCTGCGTTGATACC10610.3439578821790267No Hit
ATCAACGCAGAGTACTTTTTTTTTT10600.3436336994437024No Hit
GTACTTAGATGTTTCAGTTCCCTAG9770.3167265324117899No Hit
GGTATCAACGCAGAGTACATGGGGG9460.30667686761673824No Hit
ATCAACGCAGAGTACATGGGAAGCA9300.3014899438515502No Hit
ACTTAGATGTTTCAGTTCCCTAGGT7390.23957104140461893No Hit
GGAAGCAGTGGTATCAACGCAGAGT7250.2350324831100795No Hit
GGGTAACAGAGGTTCGCTCTGCTGG7200.23341156943345823No Hit
GTACATGGTAAGCAGTGGTATCAAC6440.20877368154881543No Hit
CCTCTAGGTACTTAGATGTTTCAGT6100.19775146854779102No Hit
CCACACACCCTATGTATTCAGATGT5910.1915919965766303No Hit
GTGGATGCCTTGGCAGTCGAACCGA5820.1886743519587121No Hit
ATTCAGATGTGGGTAACAGAGGTTC5670.18381161092884837No Hit
CTGCGTTGATACCACTGCTTCCCAT5620.18219069725222714No Hit
CTCTGCGTTGATACCACTGCTTCCC5580.18089396631093016No Hit
TGGGAAGCAGTGGTATCAACGCAGA5500.17830050442833617No Hit
TCAACGCAGAGTACATGGGAAGCAG5490.17797632169301192No Hit
CTGCTGGGTTTCCCCATTCGGACAT5370.17408612886912095No Hit
AAAAAGTACTCTGCGTTGATACCAC5250.17019593604522998No Hit
ACCATGTACTCTGCGTTGATACCAC5170.167602474162636No Hit
AACGCAGAGTACTTTTTTTTTTTTT5120.16598156048601476No Hit
CAACGCAGAGTACTTTTTTTTTTTT5020.1627397331327723No Hit
CACCCTATGTATTCAGATGTGGGTA4780.15495934748499035No Hit
GTATTCAGATGTGGGTAACAGAGGT4750.1539867992790176No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA4670.1513933373964236No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT4230.1371292970421567No Hit
CCCTAGGTTCCCTCCACACACCCTA4220.13680511430683245No Hit
GGTATCAACGCAGAGTACATGGGGA4200.13615674883618398No Hit
TCCATGTACTCTGCGTTGATACCAC4070.13194237327696878No Hit
AGTGGTATCAACGCAGAGTACATGG3930.12740381498242928No Hit
AACGCAGAGTACATGGGAAGCAGTG3790.12286525668788982No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3780.1225410739525656No Hit
TCCCATGTACTCTGCGTTGATACCA3770.12221689121724134No Hit
AAAGTACTCTGCGTTGATACCACTG3770.12221689121724134No Hit
CCCCATGTACTCTGCGTTGATACCA3730.12092016027594435No Hit
ACTGCTTCCCATGTACTCTGCGTTG3720.1205959775406201No Hit
ATGTACTCTGCGTTGATACCACTGC3670.11897506386399886No Hit
TCCCTAGGTTCCCTCCACACACCCT3630.11767833292270187No Hit
TCAACGCAGAGTACTTTTTTTTTTT3530.11443650556945939No Hit
CCTCCACACACCCTATGTATTCAGA3510.11378814009881089No Hit
GGTAAGCAGTGGTATCAACGCAGAG3500.11346395736348666No Hit
TCCCCGGATCAATGTTTGCTTCCAA3410.11054631274556842No Hit
TTGATACCACTGCTTCCCATGTACT3310.10730448539232595No Hit
GTACATGGGTAAGCAGTGGTATCAA3250.10535938898038047No Hit
TAACAGAGGTTCGCTCTGCTGGGTT3210.10406265803908347No Hit
CAACGCAGAGTACATGGGAAGCAGT3190.10341429256843497No Hit
GCCTCGGGGAGTTGGAAGCAAACAT3110.100820830685841No Hit
ATGTATTCAGATGTGGGTAACAGAG3090.10017246521519249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTCC402.7780537E-416.6045748
GTCCTTT350.002182750716.26570511
TGTCCTT350.002182750716.26570510
TGGAGGT456.581058E-414.8245064
GGAGGTG456.581058E-414.8245065
GAGGTGT456.778673E-414.7668046
GTGTCCT456.8037305E-414.7596239
CCTCTAG1203.6379788E-1214.2973871
GAAAACA1006.184564E-1014.2973861
TTCCTCT400.00515718914.2950594
AGGTGTC400.005303835514.23487
CTGGAGG500.001459630113.3420543
TTAACGC600.00591142511.06971716
TCTGTTA909.5761614E-510.54258818
CTCTAGG1658.60382E-1010.3980992
CGAACCG1303.8614417E-710.218218
GAACAAA1502.2868335E-810.167031
GAACCGA1252.568433E-69.87266319
AAAAGTA900.00107501539.5315912
GGGTAAG1106.866697E-59.4898667
Produced by FastQC (version 0.11.5)