FastQCFastQC Report
Wed 25 May 2016
SRR1294797_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294797_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118034
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39080.1253353876192498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.013.69851
GTACGAC707.2595667E-613.5729793
TCGGACG707.2825114E-613.5688339
CTTAACG604.088466E-412.6687252
TGGACCG550.00306479112.0934585
CGACCAT658.060902E-411.68650156
CGGACGT907.483086E-611.60889110
AAGACCG600.00586868811.0856695
GCGCACT600.005890502611.08014511
TGACTCG951.3581219E-511.0023185
TACGACC803.765063E-410.6899244
ATACCGT803.790319E-410.6821936
GTCCTAA2500.010.6420721
GTGCTAG3150.010.55761051
GTATCAA27500.010.4002071
CGAATTA1201.5373535E-610.285233515
GGGTACG856.585103E-410.0607821
AGACCGT1701.5752448E-910.0538286
TAGGACC1304.229578E-69.5021554
GTATTAA8300.09.501851
Produced by FastQC (version 0.11.5)