FastQCFastQC Report
Wed 25 May 2016
SRR1294797_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294797_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118034
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT114680.36779586110991735No Hit
GGTATCAACGCAGAGTACTTTTTTT71680.2298884489392996No Hit
TATCAACGCAGAGTACTTTTTTTTT66280.2125698436899662No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31660.10153834114701764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.013.712661
CATACCG550.002989734312.1335585
TCGTCGC550.003101564712.07421310
GCGCGTC600.00593866411.0680299
GTATCAA41150.010.6597211
GTCTCGC2001.8189894E-1210.4893981
CGGGACT1002.3089919E-510.4868624
GCGGGAC951.5875943E-410.03527453
GTATTAC3350.09.9627921
GCTAGGC1304.055815E-69.5335113
CTCGTTG1002.7944514E-49.48718712
CGGGACC1054.3391998E-49.0795354
GTCTTAG2553.6379788E-128.9748851
TCCCGCA850.0072245498.9730052
GCCCGTT850.0075180838.9295477
GTCGCCC2553.6379788E-128.92911611
CTCTTAG2900.08.8781731
GTACTAG2159.713403E-108.8705261
GATCTAA1752.4806286E-78.718461
ACACCGT1107.205972E-48.6292816
Produced by FastQC (version 0.11.5)