FastQCFastQC Report
Wed 25 May 2016
SRR1294796_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294796_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671273
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC45630.17081743423453913No Hit
CCCATGTACTCTGCGTTGATACCAC40900.15311052071428116No Hit
GTATCAACGCAGAGTACTTTTTTTT26980.10100053420223241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC400.00529173814.2453998
GGTACGA550.003063921112.0938132
TACATCG550.003063921112.0938135
ATACCGT658.059903E-411.686566
CGAGTAA600.005892493811.07954710
TACACCG803.764222E-410.6900655
GACCGCG750.002550948910.17933219
GTAGCAC2250.010.1353853
TAGGACG856.581696E-410.0612384
ACACCGC1151.0330414E-59.908176
GTCCTAC2251.8189894E-129.7134421
GTCCTAA2700.09.502281
GTCTTAA2054.2200554E-109.2705181
CATGCCG1054.2951704E-49.0886919
TGTACCG1054.483359E-49.0497915
GAGTAGG2950.09.01911451
CAGGACT4750.09.0021614
TAGACAG3550.08.8331065
GTGCACG1201.7598462E-48.7104241
GCGCTCA1201.7709829E-48.7053589
Produced by FastQC (version 0.11.5)