FastQCFastQC Report
Wed 25 May 2016
SRR1294796_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294796_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671273
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82160.30756871349352916No Hit
GGTATCAACGCAGAGTACTTTTTTT53570.20054109033408418No Hit
TATCAACGCAGAGTACTTTTTTTTT47450.17763066522964893No Hit
GTACATGGGAAGCAGTGGTATCAAC46350.17351277836447268No Hit
CCCATGTACTCTGCGTTGATACCAC42250.15816429095790657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT500.001516126213.28118510
GCGGAAC500.001516126213.28118511
AATCGGA600.00593895911.06786217
GTCCTAG3350.010.8185871
ATACCGT803.8044434E-410.6777776
AACCGCT803.8215934E-410.67258115
GTCTACG909.14782E-510.5971541
AGACCGT909.6082746E-510.5459536
GTAGCAC2650.010.4342523
GTCTAGC1909.276846E-1110.0394091
CGTACCC800.00438320179.5347483
GCGTCTC1002.795969E-49.4865619
ATCGTGC1106.9463655E-59.486568
GTGCTAG3000.09.2195241
CGTGCAG1651.0897929E-79.19908810
CTAGACA2700.09.1816094
CCTACGA1054.3281674E-49.08173752
TTGGCGT1054.3330557E-49.0807114
CCGCGGC1054.558175E-49.0348214
CCCGCGG1054.558175E-49.0348213
Produced by FastQC (version 0.11.5)