FastQCFastQC Report
Wed 25 May 2016
SRR1294795_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294795_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4538888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC93840.20674667451587259No Hit
CCCATGTACTCTGCGTTGATACCAC89780.19780175232347658No Hit
ATTCCATTCCATTCCATTCCATTCC58710.1293488625407809No Hit
GAATGGAATGGAATGGAATGGAATG47740.10517994715886357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG704.456142E-714.9242099
GTACCGT500.001505881813.2934716
CGGACGT853.9604583E-612.29065910
GTCGTAC907.4596137E-611.6124621
TCGTCCG1408.40555E-910.853979
CGTCCGC1409.7439624E-810.17570910
TCGTCTA1606.2391337E-910.08834914
TCGTACC951.6436547E-410.0010522
AACACCG1054.1006944E-59.9541985
CGTACCC900.00111342059.50089553
CTAGGAC3800.09.5008953
ACCGTCT2002.6739144E-109.4972248
GTCTTAG3750.09.3744231
TAGAACC2800.09.162694
CGGGACT1804.2105967E-88.9741574
TGACTCG1804.2127795E-88.9738615
TTGCGTG1804.260619E-88.9676216
GCGTCGC1606.8016743E-78.901484514
GTCTCGC2401.0186341E-108.7093461
ACCGGAC1107.1422907E-48.63717653
Produced by FastQC (version 0.11.5)