FastQCFastQC Report
Wed 25 May 2016
SRR1294795_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294795_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4538888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT108680.23944190735704426No Hit
GTACATGGGAAGCAGTGGTATCAAC95150.20963284399174426No Hit
CCCATGTACTCTGCGTTGATACCAC93670.20637213343885108No Hit
GGTATCAACGCAGAGTACTTTTTTT65210.1436695507798386No Hit
TATCAACGCAGAGTACTTTTTTTTT63610.1401444582902244No Hit
ATTCCATTCCATTCCATTCCATTCC56790.12511875155324387No Hit
GAATGGAATGGAATGGAATGGAATG49680.1094541218025208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT701.10035835E-412.20406156
ATCGTCG951.0581225E-611.9840139
TCTCGTA1053.5053854E-610.84805519
TCGGACG700.001509047810.8426779
GTCGTAG1201.455277E-610.3296161
ACCGTCC856.679502E-410.0454228
TACAGCG1054.1675412E-59.9395597
TCGTCGC1451.6513332E-79.81449210
ACGGGTC900.00107989019.5327085
CTAAGAC5500.09.5327073
CGGGTCT1106.905431E-59.4920486
ACACCGT1701.6905688E-89.4920486
ATACCGC800.004566579.4877617
CGGACGT900.00112805129.48734310
TCGTCCG1356.9714915E-69.1359599
GCGGGAC2201.5643309E-109.0994033
ACCGGCC1054.5559835E-49.0355648
ACCGTCT2002.7248461E-99.0129768
CCGTCCC1606.8804366E-78.8943839
GTATAGC3350.08.8234611
Produced by FastQC (version 0.11.5)