FastQCFastQC Report
Wed 25 May 2016
SRR1294794_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294794_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171913
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC64910.2988609580586331No Hit
CCCATGTACTCTGCGTTGATACCAC55990.25779117303501564No Hit
GAGTACATGGGAAGCAGTGGTATCA29710.13679185123897689No Hit
CATGTACTCTGCGTTGATACCACTG22030.10143131884196098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGC350.00216473116.2922844
CTTCGCG400.005290936714.24555313
CGAACAT853.9744755E-612.28602915
TGAACGG550.003074705412.08797210
AGTCCGC752.0850192E-411.3938146
GCGGGAC855.3067863E-511.1802043
TTCGCGG600.005905784711.07604214
CCCGTCA951.3654846E-510.99656812
GACGAAC951.3654846E-510.99656813
GTTAGAC700.001487661510.860773
TCGTCCG700.001494839210.85450659
TCTAGTA1502.1209416E-910.7705132
TGACGAA1002.4078494E-510.44673912
TGCGTGA1151.0345158E-59.90675117
CTTGCGT1151.0347994E-59.90652215
TAGTACG1502.6334237E-79.5038324
CGTCCGC1106.8562214E-59.49769210
AGACCGT800.0045369319.4948446
TCGTCTA800.00454124859.4937514
TAATACT3450.09.3660964
Produced by FastQC (version 0.11.5)