FastQCFastQC Report
Wed 25 May 2016
SRR1294794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171913
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC66970.30834568419637437No Hit
GTATCAACGCAGAGTACTTTTTTTT62500.2877647493246737No Hit
CCCATGTACTCTGCGTTGATACCAC59610.27445850731590077No Hit
GGTATCAACGCAGAGTACTTTTTTT40140.18481403260627843No Hit
TATCAACGCAGAGTACTTTTTTTTT36330.1672718934874463No Hit
GAGTACATGGGAAGCAGTGGTATCA30930.1424090191457945No Hit
CATGTACTCTGCGTTGATACCACTG22490.10354926739699058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGC350.002188505116.2656679
ACGGGTC653.266614E-614.6608465
ACGTCGT500.001465183913.341375
TGAACGG655.5178287E-513.13765310
TCGTCTA604.1343336E-412.65165614
GTCGGCC604.1343336E-412.65165612
CACGGGT802.7778708E-511.9119374
CCAAGCG752.0984065E-411.3859669
CTAGTAG1104.775411E-711.2621943
AGACCGT855.3643584E-511.1683686
TAATCGG600.00592902611.07019916
ACCGAGT600.00593105811.0696898
CCTAGTA1657.6397555E-1110.9741822
CGGAACC700.001453656510.8909134
CGGGTCT803.7973313E-410.6797526
ACCGCCG803.813525E-410.67483511
CTAGACT2250.010.58838754
GTCCTAA1553.4688128E-910.454181
GCCTAGA1409.2308255E-810.2125931
CCTAGAC2151.8189894E-1210.1944023
Produced by FastQC (version 0.11.5)