FastQCFastQC Report
Wed 25 May 2016
SRR1294793_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294793_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361947
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC79720.33751815768939775No Hit
CCCATGTACTCTGCGTTGATACCAC72160.30551066556531536No Hit
GAGTACATGGGAAGCAGTGGTATCA37420.15842861842369876No Hit
CATGTACTCTGCGTTGATACCACTG27420.11609066587861624No Hit
GCGTTGATACCACTGCTTCCCATGT26060.11033270433248503No Hit
GTATCAACGCAGAGTACTTTTTTTT24120.10211914153873901No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA250.00604817718.99172818
TACGGTC500.001496480313.3040865
TAGTACG1153.765308E-1013.2206112
AGTACGG1155.30963E-912.3943223
GGGCCGT951.3668519E-510.9956776
TAGCCCG803.7615388E-410.69078355
GTCTAGA1909.094947E-1210.502781
GGATACG1002.392815E-510.4527661
GTATTAG2950.010.3078141
CGTTAAT750.00264262910.1360161
GCACCGA750.002657088710.1293516
CGACGAC856.6195126E-410.0548716
GTACGGT951.6391996E-410.0032844
TAGGACC1151.0228592E-59.91634
GTCCTAA1752.562956E-99.7740161
GTGTAAG3250.09.6487071
TAGACTG2500.09.5029195
CTAGCAC2401.8189894E-129.5023143
GTCACCG800.00453713269.49485715
GTATTAT4550.09.3980911
Produced by FastQC (version 0.11.5)