FastQCFastQC Report
Wed 25 May 2016
SRR1294793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361947
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC80720.34175195294390603No Hit
CCCATGTACTCTGCGTTGATACCAC75290.3187624447119262No Hit
GTATCAACGCAGAGTACTTTTTTTT67980.28781340140147094No Hit
GGTATCAACGCAGAGTACTTTTTTT42590.1803173398895064No Hit
TATCAACGCAGAGTACTTTTTTTTT38190.16168864076967013No Hit
GAGTACATGGGAAGCAGTGGTATCA36600.154956906315002No Hit
CATGTACTCTGCGTTGATACCACTG27070.11460883753953836No Hit
GCGTTGATACCACTGCTTCCCATGT25920.10973997299685387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCA400.005325632614.2315999
CGAATTA604.1360696E-412.65111315
GCGCTCG600.005933051511.06925511
TCAGGAC3250.010.8505753
GTATTAC2450.010.5054641
TACATCG750.00257801410.1663055
GTATTAG2650.010.0723241
TAGACCG951.5915983E-410.0325375
TTAGACA1909.458745E-1110.0325374
CTAGGAC2201.8189894E-129.9641333
TGGCGTA1159.879675E-69.9452985
AGGACCG1159.879675E-69.9452985
GTCCTAA1856.0936145E-109.7903761
TATACTG4450.09.6385
GTTCTAA2303.6379788E-129.5327351
GTCTAGC1304.058469E-69.5327351
CTTATAC2002.4920155E-109.53091053
CTTAGAC1401.0131316E-69.53091053
GTCTTGC2950.09.3711631
ATAGGAC1856.337359E-99.2733183
Produced by FastQC (version 0.11.5)