FastQCFastQC Report
Wed 25 May 2016
SRR1294792_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294792_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430478
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC189894.411142962009674No Hit
CCCATGTACTCTGCGTTGATACCAC179474.169086457379936No Hit
GAGTACATGGGAAGCAGTGGTATCA92602.151097152467722No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA88722.0609647879798736No Hit
CATGTACTCTGCGTTGATACCACTG77171.7926583936925928No Hit
GCGTTGATACCACTGCTTCCCATGT63021.4639540232021149No Hit
ACGCAGAGTACATGGGAAGCAGTGG60061.3951932502938593No Hit
GGTATCAACGCAGAGTACATGGGAA56661.3162112814127551No Hit
TATCAACGCAGAGTACATGGGAAGC55121.2804370955077844No Hit
GTATCAACGCAGAGTACATGGGAAG53061.232583314362174No Hit
ACTCTGCGTTGATACCACTGCTTCC51851.20447502543684No Hit
GTGGTATCAACGCAGAGTACATGGG39850.9257151352682368No Hit
GTATCAACGCAGAGTACTTTTTTTT38730.899697545519167No Hit
GCTTCCCATGTACTCTGCGTTGATA33080.7684480972314497No Hit
GTACTCTGCGTTGATACCACTGCTT32970.7658927982382375No Hit
GGTATCAACGCAGAGTACTTTTTTT30050.6980612249638773No Hit
ATACCACTGCTTCCCATGTACTCTG28790.668791436496174No Hit
GATACCACTGCTTCCCATGTACTCT27780.6453291457403165No Hit
CAGTGGTATCAACGCAGAGTACATG27040.6281389525132527No Hit
GGGAAGCAGTGGTATCAACGCAGAG26380.6128071585539795No Hit
CATGGGAAGCAGTGGTATCAACGCA24400.56681177667616No Hit
CTGCTTCCCATGTACTCTGCGTTGA24350.5656502771337908No Hit
TATCAACGCAGAGTACTTTTTTTTT23850.5540352817100991No Hit
GTTGATACCACTGCTTCCCATGTAC22770.5289468915949247No Hit
GCAGAGTACATGGGAAGCAGTGGTA22610.5252300930593433No Hit
GCAGTGGTATCAACGCAGAGTACAT21930.5094336992831225No Hit
ACATGGGAAGCAGTGGTATCAACGC21510.49967710312722136No Hit
TACCACTGCTTCCCATGTACTCTGC21480.4989802034017998No Hit
CTTCCCATGTACTCTGCGTTGATAC19460.45205562189008497No Hit
CCACTGCTTCCCATGTACTCTGCGT19010.4416021260087623No Hit
AAGCAGTGGTATCAACGCAGAGTAC17120.39769744330720735No Hit
ATGGGAAGCAGTGGTATCAACGCAG16790.39003154632757076No Hit
CCATGTACTCTGCGTTGATACCACT15890.3691245545649255No Hit
TGATACCACTGCTTCCCATGTACTC14420.33497646801927156No Hit
TTCCCATGTACTCTGCGTTGATACC14420.33497646801927156No Hit
ATCAACGCAGAGTACATGGGAAGCA11480.2666802949279638No Hit
AAACAAAAAAAAAAAAAAAAAAAAA11190.2599435975822226No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA10660.24763170243310922No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10630.24693480270768772No Hit
GTACATGGTAAGCAGTGGTATCAAC9630.2237048118603041No Hit
GGAAGCAGTGGTATCAACGCAGAGT8570.19908102156207752No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7900.18351692769433048No Hit
ACCATGTACTCTGCGTTGATACCAC7520.17468953117232472No Hit
CTCTGCGTTGATACCACTGCTTCCC7220.16772053391810965No Hit
CTGCGTTGATACCACTGCTTCCCAT6920.16075153666389455No Hit
AAAAAGTACTCTGCGTTGATACCAC6580.15285333977578414No Hit
TCAACGCAGAGTACATGGGAAGCAG6370.14797504169783357No Hit
TCCATGTACTCTGCGTTGATACCAC5940.13798614563345862No Hit
TGGGAAGCAGTGGTATCAACGCAGA5620.13055254856229587No Hit
AGTGGTATCAACGCAGAGTACATGG5540.12869414929450518No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5380.1249773507589238No Hit
AAAGTACTCTGCGTTGATACCACTG5320.12358355130808078No Hit
GTGGTATCAACGCAGAGTACTTTTT5310.12335125139960694No Hit
AAAAACAAAAAAAAAAAAAAAAAAA5250.12195745194876392No Hit
GGTAAGCAGTGGTATCAACGCAGAG5030.11684685396233954No Hit
ACTGCTTCCCATGTACTCTGCGTTG4920.11429155496912734No Hit
AACGCAGAGTACATGGGAAGCAGTG4900.11382695515217967No Hit
TCCCATGTACTCTGCGTTGATACCA4860.11289775551828432No Hit
CCCCATGTACTCTGCGTTGATACCA4740.1101101566165983No Hit
GGTATCAACGCAGAGTACATGGGGG4530.10523185853864774No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4500.10453495881322622No Hit
TTGATACCACTGCTTCCCATGTACT4370.10151506000306637No Hit
GTACATGGAAAGCAGTGGTATCAAC4350.1010504601861187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGAA350.002156495316.2972972
GAACAAA3950.015.4033511
TCAGGAT400.005250126614.2584733
ATCACAT400.005294924614.24022915
GTACAAA604.056911E-412.6756751
GTAAAAA550.003045969612.0995071
CTTAGAC550.00304862912.0980993
GACATTA550.003072652712.0854297
ACGCAAA1209.822543E-911.8834461
CGCAAAA1209.822543E-911.8834462
TATAAAG750.00262537310.1405392
TAATCCC856.536443E-410.0648045
CTACAAA800.0044778829.5067571
CCTGTAA900.00110286529.5067561
GTAATCC900.00110404149.5056494
CTGTAAT1054.4429547E-49.0540542
AATCCCA950.0018292618.9938296
CATGGTA3000.08.8719394
CCAAAAC1554.141386E-68.5857473
TAACAAA1000.00287704128.5560811
Produced by FastQC (version 0.11.5)