##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294792_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 430478 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.059696430479608 33.0 31.0 34.0 16.0 34.0 2 30.26358606014709 33.0 31.0 34.0 20.0 34.0 3 30.53700537541988 34.0 31.0 34.0 23.0 34.0 4 33.31348408048727 37.0 35.0 37.0 19.0 37.0 5 34.17957015224936 37.0 35.0 37.0 28.0 37.0 6 33.97246316884951 37.0 35.0 37.0 27.0 37.0 7 34.15850287354987 37.0 35.0 37.0 28.0 37.0 8 34.11213116582032 37.0 35.0 37.0 27.0 37.0 9 35.32572628566384 39.0 37.0 39.0 23.0 39.0 10 35.66277254586762 39.0 37.0 39.0 27.0 39.0 11 35.53541876704501 39.0 37.0 39.0 27.0 39.0 12 35.67281022491277 39.0 37.0 39.0 27.0 39.0 13 35.2884258893602 39.0 37.0 39.0 24.0 39.0 14 36.70278852810132 41.0 38.0 41.0 25.0 41.0 15 36.65426340022022 41.0 38.0 41.0 25.0 41.0 16 36.62680090504044 41.0 38.0 41.0 26.0 41.0 17 36.71904719869541 41.0 38.0 41.0 26.0 41.0 18 36.713413925914914 41.0 38.0 41.0 26.0 41.0 19 36.73644181584192 41.0 38.0 41.0 26.0 41.0 20 36.80950478305512 41.0 38.0 41.0 26.0 41.0 21 36.8058692894875 41.0 38.0 41.0 27.0 41.0 22 36.88361774585461 41.0 38.0 41.0 27.0 41.0 23 36.957516992738306 41.0 38.0 41.0 27.0 41.0 24 37.13641579825217 41.0 38.0 41.0 27.0 41.0 25 36.33140137242786 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22954877655440953 1101 2 0.14500914392339936 1101 3 0.06193957136841277 1101 4 0.2634014445999924 1101 5 0.2119291568353887 1101 6 0.12079803411966594 1101 7 0.10218798287649378 1101 8 0.062016507282073974 1101 9 0.2546447381285972 1101 10 0.34542296005358963 1101 11 0.4121711147274141 1101 12 0.3699781805749325 1101 13 0.29294406700959996 1101 14 0.3002599187415882 1101 15 0.2210014841692285 1101 16 0.21643856314213394 1101 17 0.1176428088466821 1101 18 -0.009072325903680678 1101 19 0.08074105424326916 1101 20 -0.051781010174195785 1101 21 -0.2465541349964937 1101 22 -0.08330813237820678 1101 23 0.0960437227861135 1101 24 -0.1964247618272026 1101 25 -0.17323093352640484 1102 1 -0.0032889788796772734 1102 2 -0.029234295389045428 1102 3 -0.00832001228052448 1102 4 0.02365640698729976 1102 5 0.03981313855815927 1102 6 -0.2888152838641531 1102 7 0.18782625020417498 1102 8 0.15188774088548485 1102 9 0.3552187042922128 1102 10 0.3573780818607517 1102 11 0.11237428617075551 1102 12 0.32869855596356956 1102 13 0.4697469743027227 1102 14 0.3213235745697105 1102 15 0.4238475250605589 1102 16 0.4505396457205251 1102 17 0.17776843059778713 1102 18 0.39780925221614893 1102 19 0.21546040146955647 1102 20 6.22456045476838E-4 1102 21 0.012474415650075343 1102 22 0.09740780769305246 1102 23 -0.10355376259128235 1102 24 -0.11163965049308189 1102 25 -0.27269743758299825 1103 1 -0.39886390956696616 1103 2 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20 0.37144729180638336 2213 21 0.5889376656894569 2213 22 0.1406038599941013 2213 23 0.3397321262583404 2213 24 0.31164802657220037 2213 25 0.3651718608829668 2214 1 0.003495397792342203 2214 2 0.025851846933051092 2214 3 -0.3574971123340305 2214 4 0.08048037702474886 2214 5 -0.09130082612883683 2214 6 0.05354533678860207 2214 7 0.2966502202416237 2214 8 -0.10093408897005673 2214 9 -0.1715335696510749 2214 10 -0.4325759042280666 2214 11 -0.28709066835038044 2214 12 -0.4043886564494841 2214 13 -0.3889389540011834 2214 14 -0.0923329262328565 2214 15 -0.2546350859613753 2214 16 0.0016878533181667876 2214 17 -0.3827387925161787 2214 18 0.024906095447825294 2214 19 0.16791197985372008 2214 20 0.16175675246067556 2214 21 0.09363439424825515 2214 22 0.18185938607721397 2214 23 0.08923168417169336 2214 24 0.10993980376053258 2214 25 0.003445954605339807 2215 1 0.1526077166329216 2215 2 0.12313807881711014 2215 3 0.5604304238978557 2215 4 0.29563979731460677 2215 5 0.35338395647985976 2215 6 0.22922287302048971 2215 7 0.33345094487930993 2215 8 0.13827243276907808 2215 9 -0.0121132797959973 2215 10 -0.02860488973531261 2215 11 -0.030659508930092727 2215 12 -0.21712416369586407 2215 13 -0.06344620037799586 2215 14 0.09306924768019087 2215 15 0.09323085606760628 2215 16 0.019499447521063473 2215 17 0.248880772701213 2215 18 0.3053372548681139 2215 19 0.3411800957957496 2215 20 0.4058147234751672 2215 21 0.3659097565670919 2215 22 0.39024706723663627 2215 23 0.561282408809376 2215 24 0.3873745863692264 2215 25 0.28252928793867227 2216 1 0.03262279744330243 2216 2 0.2462672046991976 2216 3 0.22680306218603974 2216 4 0.044672349101539055 2216 5 0.25779865031095284 2216 6 0.1002748306804051 2216 7 0.03999402477914771 2216 8 -0.07221157588104887 2216 9 -0.07642013160569405 2216 10 0.021190238878389778 2216 11 0.10723742938085934 2216 12 0.3667806280182262 2216 13 -0.04281678995231886 2216 14 0.3167560790027437 2216 15 0.28852023690075157 2216 16 0.12196359502846121 2216 17 0.29282141690790553 2216 18 0.2756949261633608 2216 19 0.08607253831794281 2216 20 0.3153867699126778 2216 21 0.15332723892539235 2216 22 0.03739167229013418 2216 23 0.1802927661961249 2216 24 0.4185890184202137 2216 25 0.2489887120224452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 428.0 3 68.0 4 44.0 5 39.0 6 37.0 7 118.0 8 46.0 9 101.0 10 207.0 11 378.0 12 706.0 13 1311.0 14 2433.0 15 4135.0 16 5873.0 17 6851.0 18 6770.0 19 6357.0 20 5655.0 21 5018.0 22 4211.0 23 3668.0 24 3273.0 25 2787.0 26 2511.0 27 2268.0 28 2026.0 29 1901.0 30 2297.0 31 2726.0 32 3409.0 33 4547.0 34 6289.0 35 9880.0 36 17863.0 37 43966.0 38 270281.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.488341836497625 23.773546330629046 11.711831014710938 21.026280818162387 2 12.064202139916164 29.68464515888773 32.815616686411744 25.435536014784365 3 19.775009426979317 37.47422128495547 25.278503228449463 17.47226605961575 4 12.3737109263177 27.83235467301574 27.278672652669844 32.51526174799672 5 9.49649665960567 46.29414092506809 30.73488675248493 13.474475662841314 6 26.687223644403073 38.923900395624855 16.742611124040028 17.646264835932048 7 18.40533061496175 43.06268313515753 20.49019128678122 18.041794963099502 8 24.684087699255677 39.94257620635338 19.254886722958116 16.118449371432824 9 24.774259011021744 25.097510798331847 17.95851020256181 32.1697199880846 10 16.480309095987337 38.59533562982963 26.406759147193 18.51759612699004 11 23.275460916341707 35.16482238085045 16.52853912088526 25.031177581922588 12 21.596559852378945 39.99683536203691 24.00703666558852 14.399568119995626 13 26.94096451166883 29.33385474132112 20.923124909801075 22.80205583720898 14 20.15587401618781 35.365808250292055 22.94499029569054 21.533327437829584 15 31.008908292065385 28.75088431321443 20.718295044122577 19.52191235059761 16 15.953317353564032 34.547020083312 30.15545367806195 19.34420888506202 17 22.745593580846794 36.0716662710547 24.284058699507113 16.898681448591386 18 22.427868615924524 29.28331727569339 28.72292891352612 19.56588519485597 19 20.77455531125304 35.925266696913646 29.3172326341628 13.982945357670518 20 23.021694912098603 29.646532584259205 26.99250150109615 20.339271002546045 21 19.386776644075372 32.68786772920235 29.376675728010724 18.54867989871155 22 22.13481316022151 31.671320210340177 21.82837730932105 24.36548932011727 23 17.464388193705886 31.97161760564855 28.06938746064142 22.49460674000414 24 20.671124382370536 37.592135304782545 23.94412417057861 17.792616142268315 25 20.987016267083245 28.639982039793082 22.80338250563595 27.56961918748772 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10123.0 1 10123.0 2 8495.0 3 6867.0 4 6867.0 5 6867.0 6 6882.5 7 6898.0 8 6898.0 9 6898.0 10 7005.0 11 7112.0 12 7112.0 13 7112.0 14 7391.5 15 7671.0 16 7671.0 17 7671.0 18 9082.5 19 10494.0 20 10494.0 21 10494.0 22 12381.5 23 14269.0 24 14269.0 25 14269.0 26 16964.5 27 19660.0 28 19660.0 29 19660.0 30 21254.0 31 22848.0 32 22848.0 33 22848.0 34 23244.0 35 23640.0 36 23640.0 37 23640.0 38 23031.0 39 22422.0 40 22422.0 41 22422.0 42 25753.5 43 29085.0 44 29085.0 45 29085.0 46 71688.0 47 114291.0 48 114291.0 49 114291.0 50 96356.0 51 78421.0 52 78421.0 53 78421.0 54 54260.0 55 30099.0 56 30099.0 57 30099.0 58 20941.5 59 11784.0 60 11784.0 61 11784.0 62 9754.0 63 7724.0 64 7724.0 65 7724.0 66 5874.5 67 4025.0 68 4025.0 69 4025.0 70 2911.0 71 1797.0 72 1797.0 73 1797.0 74 1311.5 75 826.0 76 826.0 77 826.0 78 573.5 79 321.0 80 321.0 81 321.0 82 194.0 83 67.0 84 67.0 85 67.0 86 50.5 87 34.0 88 34.0 89 34.0 90 17.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011614995423691803 2 0.1939704235756531 3 0.199777921287499 4 0.18793062595533339 5 0.2062823187247664 6 0.18072932879264447 7 0.18793062595533339 8 0.1605192367554207 9 0.1816585284265398 10 0.1946673233010746 11 0.15842853757915618 12 0.1693466332774265 13 0.20256552018918503 14 0.17933552934180144 15 0.17794172989095844 16 0.1784063297079061 17 0.17747713007401075 18 0.1802647289756968 19 0.15866083748763005 20 0.18398152751127816 21 0.18909212549770255 22 0.16214533611473758 23 0.18003242906722294 24 0.18839522577228104 25 0.6657715376860142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 430478.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.40393763806575 #Duplication Level Percentage of deduplicated Percentage of total 1 79.78059333405415 29.841083377948447 2 12.088629123508882 9.043246597308633 3 3.966854400854352 4.451279238865284 4 1.6297486591923018 2.4383606885660045 5 0.8034587333640143 1.502626017875344 6 0.44116263851973153 0.9900731891661947 7 0.2474126567067748 0.6477945307629861 8 0.18475976716654005 0.5528594247296649 9 0.12183271594402259 0.4101320978501759 >10 0.5777606962903281 3.9911021085526492 >50 0.0627421839145309 1.6971348297196402 >100 0.06087855468934682 4.925269302514679 >500 0.009939355867648459 2.418835374778403 >1k 0.0173938727683848 14.606884438047027 >5k 0.005590887675552258 13.900984162487296 >10k+ 0.0012424194834560573 8.582334620827579 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 18989 4.411142962009674 No Hit CCCATGTACTCTGCGTTGATACCAC 17947 4.169086457379936 No Hit GAGTACATGGGAAGCAGTGGTATCA 9260 2.151097152467722 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 8872 2.0609647879798736 No Hit CATGTACTCTGCGTTGATACCACTG 7717 1.7926583936925928 No Hit GCGTTGATACCACTGCTTCCCATGT 6302 1.4639540232021149 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6006 1.3951932502938593 No Hit GGTATCAACGCAGAGTACATGGGAA 5666 1.3162112814127551 No Hit TATCAACGCAGAGTACATGGGAAGC 5512 1.2804370955077844 No Hit GTATCAACGCAGAGTACATGGGAAG 5306 1.232583314362174 No Hit ACTCTGCGTTGATACCACTGCTTCC 5185 1.20447502543684 No Hit GTGGTATCAACGCAGAGTACATGGG 3985 0.9257151352682368 No Hit GTATCAACGCAGAGTACTTTTTTTT 3873 0.899697545519167 No Hit GCTTCCCATGTACTCTGCGTTGATA 3308 0.7684480972314497 No Hit GTACTCTGCGTTGATACCACTGCTT 3297 0.7658927982382375 No Hit GGTATCAACGCAGAGTACTTTTTTT 3005 0.6980612249638773 No Hit ATACCACTGCTTCCCATGTACTCTG 2879 0.668791436496174 No Hit GATACCACTGCTTCCCATGTACTCT 2778 0.6453291457403165 No Hit CAGTGGTATCAACGCAGAGTACATG 2704 0.6281389525132527 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2638 0.6128071585539795 No Hit CATGGGAAGCAGTGGTATCAACGCA 2440 0.56681177667616 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2435 0.5656502771337908 No Hit TATCAACGCAGAGTACTTTTTTTTT 2385 0.5540352817100991 No Hit GTTGATACCACTGCTTCCCATGTAC 2277 0.5289468915949247 No Hit GCAGAGTACATGGGAAGCAGTGGTA 2261 0.5252300930593433 No Hit GCAGTGGTATCAACGCAGAGTACAT 2193 0.5094336992831225 No Hit ACATGGGAAGCAGTGGTATCAACGC 2151 0.49967710312722136 No Hit TACCACTGCTTCCCATGTACTCTGC 2148 0.4989802034017998 No Hit CTTCCCATGTACTCTGCGTTGATAC 1946 0.45205562189008497 No Hit CCACTGCTTCCCATGTACTCTGCGT 1901 0.4416021260087623 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1712 0.39769744330720735 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1679 0.39003154632757076 No Hit CCATGTACTCTGCGTTGATACCACT 1589 0.3691245545649255 No Hit TGATACCACTGCTTCCCATGTACTC 1442 0.33497646801927156 No Hit TTCCCATGTACTCTGCGTTGATACC 1442 0.33497646801927156 No Hit ATCAACGCAGAGTACATGGGAAGCA 1148 0.2666802949279638 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 1119 0.2599435975822226 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 1066 0.24763170243310922 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1063 0.24693480270768772 No Hit GTACATGGTAAGCAGTGGTATCAAC 963 0.2237048118603041 No Hit GGAAGCAGTGGTATCAACGCAGAGT 857 0.19908102156207752 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 790 0.18351692769433048 No Hit ACCATGTACTCTGCGTTGATACCAC 752 0.17468953117232472 No Hit CTCTGCGTTGATACCACTGCTTCCC 722 0.16772053391810965 No Hit CTGCGTTGATACCACTGCTTCCCAT 692 0.16075153666389455 No Hit AAAAAGTACTCTGCGTTGATACCAC 658 0.15285333977578414 No Hit TCAACGCAGAGTACATGGGAAGCAG 637 0.14797504169783357 No Hit TCCATGTACTCTGCGTTGATACCAC 594 0.13798614563345862 No Hit TGGGAAGCAGTGGTATCAACGCAGA 562 0.13055254856229587 No Hit AGTGGTATCAACGCAGAGTACATGG 554 0.12869414929450518 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 538 0.1249773507589238 No Hit AAAGTACTCTGCGTTGATACCACTG 532 0.12358355130808078 No Hit GTGGTATCAACGCAGAGTACTTTTT 531 0.12335125139960694 No Hit AAAAACAAAAAAAAAAAAAAAAAAA 525 0.12195745194876392 No Hit GGTAAGCAGTGGTATCAACGCAGAG 503 0.11684685396233954 No Hit ACTGCTTCCCATGTACTCTGCGTTG 492 0.11429155496912734 No Hit AACGCAGAGTACATGGGAAGCAGTG 490 0.11382695515217967 No Hit TCCCATGTACTCTGCGTTGATACCA 486 0.11289775551828432 No Hit CCCCATGTACTCTGCGTTGATACCA 474 0.1101101566165983 No Hit GGTATCAACGCAGAGTACATGGGGG 453 0.10523185853864774 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 450 0.10453495881322622 No Hit TTGATACCACTGCTTCCCATGTACT 437 0.10151506000306637 No Hit GTACATGGAAAGCAGTGGTATCAAC 435 0.1010504601861187 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.3229990847383606E-4 0.0 5 0.0 0.0 0.0 4.645998169476721E-4 0.0 6 0.0 0.0 0.0 4.645998169476721E-4 0.0 7 0.0 0.0 0.0 4.645998169476721E-4 0.0 8 2.3229990847383606E-4 0.0 0.0 4.645998169476721E-4 0.0 9 2.3229990847383606E-4 0.0 0.0 4.645998169476721E-4 0.0 10 2.3229990847383606E-4 0.0 0.0 6.968997254215082E-4 0.0 11 2.3229990847383606E-4 0.0 0.0 9.291996338953442E-4 0.0 12 2.3229990847383606E-4 0.0 0.0 0.0011614995423691802 0.0 13 2.3229990847383606E-4 0.0 0.0 0.0011614995423691802 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTGAA 35 0.0021564953 16.297297 2 GAACAAA 395 0.0 15.403351 1 TCAGGAT 40 0.0052501266 14.258473 3 ATCACAT 40 0.0052949246 14.240229 15 GTACAAA 60 4.056911E-4 12.675675 1 GTAAAAA 55 0.0030459696 12.099507 1 CTTAGAC 55 0.003048629 12.098099 3 GACATTA 55 0.0030726527 12.085429 7 ACGCAAA 120 9.822543E-9 11.883446 1 CGCAAAA 120 9.822543E-9 11.883446 2 TATAAAG 75 0.002625373 10.140539 2 TAATCCC 85 6.536443E-4 10.064804 5 CTACAAA 80 0.004477882 9.506757 1 CCTGTAA 90 0.0011028652 9.506756 1 GTAATCC 90 0.0011040414 9.505649 4 CTGTAAT 105 4.4429547E-4 9.054054 2 AATCCCA 95 0.001829261 8.993829 6 CATGGTA 300 0.0 8.871939 4 CCAAAAC 155 4.141386E-6 8.585747 3 TAACAAA 100 0.0028770412 8.556081 1 >>END_MODULE