FastQCFastQC Report
Wed 25 May 2016
SRR1294792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430478
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC196254.558885703799033No Hit
CCCATGTACTCTGCGTTGATACCAC187214.3488865865386845No Hit
GTATCAACGCAGAGTACTTTTTTTT121662.8261606864926896No Hit
GAGTACATGGGAAGCAGTGGTATCA99992.322766784829887No Hit
GGTATCAACGCAGAGTACTTTTTTT94062.185012939104902No Hit
TATCAACGCAGAGTACTTTTTTTTT80971.8809323589126508No Hit
CATGTACTCTGCGTTGATACCACTG76861.785457096529904No Hit
ACGCAGAGTACATGGGAAGCAGTGG68941.6014755690186258No Hit
GCGTTGATACCACTGCTTCCCATGT63331.4711553203648038No Hit
GGTATCAACGCAGAGTACATGGGAA59741.3877596532226966No Hit
TATCAACGCAGAGTACATGGGAAGC58891.3680141610024206No Hit
GTATCAACGCAGAGTACATGGGAAG55131.2806693954162582No Hit
ACTCTGCGTTGATACCACTGCTTCC54171.25836860420277No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44381.0309469938068845No Hit
GTGGTATCAACGCAGAGTACATGGG40780.9473190267563035No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39650.92106913709876No Hit
GCTTCCCATGTACTCTGCGTTGATA34380.7986470853330483No Hit
GTACTCTGCGTTGATACCACTGCTT31830.7394106086722202No Hit
CAGTGGTATCAACGCAGAGTACATG29090.6757604337503891No Hit
ATACCACTGCTTCCCATGTACTCTG28810.6692560363131217No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28670.6660038375944879No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA28640.6653069378690665No Hit
GATACCACTGCTTCCCATGTACTCT28300.657408740980956No Hit
GGGAAGCAGTGGTATCAACGCAGAG28120.653227342628427No Hit
CATGGGAAGCAGTGGTATCAACGCA27270.6334818504081509No Hit
GCAGAGTACATGGGAAGCAGTGGTA25250.5865572688964361No Hit
ACATGGGAAGCAGTGGTATCAACGC24750.5749422734727443No Hit
CTGCTTCCCATGTACTCTGCGTTGA24470.5684378760354768No Hit
GCAGTGGTATCAACGCAGAGTACAT23360.5426525861948811No Hit
GTTGATACCACTGCTTCCCATGTAC22030.5117566983678609No Hit
TACCACTGCTTCCCATGTACTCTGC20800.48318380962557905No Hit
CTTCCCATGTACTCTGCGTTGATAC20370.4731949135612041No Hit
CCACTGCTTCCCATGTACTCTGCGT19720.4580954195104047No Hit
AAGCAGTGGTATCAACGCAGAGTAC18660.4334716292121781No Hit
GCAGAGTACTTTTTTTTTTTTTTTT18370.42673493186643685No Hit
ATGGGAAGCAGTGGTATCAACGCAG17420.40466644056142237No Hit
CCATGTACTCTGCGTTGATACCACT16650.38677934760893706No Hit
GTGGTATCAACGCAGAGTACTTTTT15490.3598325582259721No Hit
TGATACCACTGCTTCCCATGTACTC14830.3445007642666989No Hit
TTCCCATGTACTCTGCGTTGATACC13850.32173537323626294No Hit
ATCAACGCAGAGTACTTTTTTTTTT12450.2892133860499259No Hit
ATCAACGCAGAGTACATGGGAAGCA12020.27922448998555094No Hit
GTACATGGTAAGCAGTGGTATCAAC9380.21789731414845823No Hit
GGAAGCAGTGGTATCAACGCAGAGT9370.21766501423998436No Hit
ACCATGTACTCTGCGTTGATACCAC7730.17956782925027526No Hit
CTCTGCGTTGATACCACTGCTTCCC7040.16353913556558058No Hit
AAAAAGTACTCTGCGTTGATACCAC6950.16144843638931605No Hit
CTGCGTTGATACCACTGCTTCCCAT6800.15796393776220852No Hit
TCAACGCAGAGTACATGGGAAGCAG6690.15540863876899633No Hit
TGGGAAGCAGTGGTATCAACGCAGA6460.1500657408740981No Hit
AGTGGTATCAACGCAGAGTACATGG5440.12637115020976683No Hit
TCCATGTACTCTGCGTTGATACCAC5400.12544195057587149No Hit
AAAGTACTCTGCGTTGATACCACTG5400.12544195057587149No Hit
CAACGCAGAGTACTTTTTTTTTTTT5310.12335125139960694No Hit
TCCCATGTACTCTGCGTTGATACCA5240.12172515204029011No Hit
AACGCAGAGTACTTTTTTTTTTTTT5230.12149285213181625No Hit
GGTAAGCAGTGGTATCAACGCAGAG5230.12149285213181625No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA5210.12102825231486858No Hit
CCCCATGTACTCTGCGTTGATACCA5100.11847295332165639No Hit
AACGCAGAGTACATGGGAAGCAGTG4970.11545305451149653No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT4880.11336235533523199No Hit
ACTGCTTCCCATGTACTCTGCGTTG4770.11080705634201979No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT4740.1101101566165983No Hit
GGTATCAACGCAGAGTACATGGGGG4680.10871635716575528No Hit
GTACATGGAAAGCAGTGGTATCAAC4470.10383805908780472No Hit
TCAACGCAGAGTACTTTTTTTTTTT4460.10360575917933088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA1600.016.6922381
ACACGGT500.001507487313.2870666
AAAAGTA1553.414243E-910.456612
TAGATGT750.002566311510.1683784
CTTAGAT856.3600147E-410.09478952
AAACATG800.0045536269.4874558
AAAAAGT1752.4716428E-89.2658951
TTAGATG950.00176162529.0311253
AACATGG950.00183987878.9881159
ACAAAAA6900.08.9802263
ACACAAA2201.4642865E-98.6712921
CACAAAA2252.2537279E-98.4746372
ATGGGTC1409.925001E-58.1710195
GAAAAAA5550.08.0775821
AAGTACT2158.050665E-87.98099474
GGGTAAG1502.3350594E-47.58996347
CATGGGT6150.07.4402774
AAAGTAC2209.847881E-77.3662973
ATGGGTT1300.003569967.33296545
AACAAAA5600.07.32072542
Produced by FastQC (version 0.11.5)