FastQCFastQC Report
Wed 25 May 2016
SRR1294791_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294791_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4908331
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAA550.003075644612.08797811
TACGACC658.015589E-411.6946094
ATTAGAC4650.010.2163323
TTAGACT4800.09.6998244
GGTATCA19200.09.6993291
TATCCCG800.00451371179.501195
TCGACCG900.00111657459.4979899
GTATCAA36350.09.3314831
GTATTAG6050.08.9517171
GTGTAGG6800.08.9424791
GTCCTAG5350.08.8797981
TAGCGTG1401.084547E-58.8225345
GTCGTAG1107.13889E-48.6376221
CTGGTCG1107.1643194E-48.6345359
GTTCTAG5350.08.5246061
TATTCCG2356.166374E-108.4904255
CCAGGAC8600.08.39643
TATGTCG1252.7417217E-48.3610475
AGACCGT1252.7608286E-48.355676
TAGACAG6400.08.3135415
Produced by FastQC (version 0.11.5)