FastQCFastQC Report
Wed 25 May 2016
SRR1294791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4908331
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105230.21439059427736232No Hit
GGTATCAACGCAGAGTACTTTTTTT62890.12812909316832952No Hit
TATCAACGCAGAGTACTTTTTTTTT59640.12150769783048454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1001.9633026E-611.3848679
CCGAAAA951.3817811E-510.9855113
CGACCGC700.001509024110.8427310
GGTATCA23700.010.7423831
TATACCG909.203012E-510.5912815
AGACCGT1451.4644684E-810.4742856
TACCGTC856.676285E-410.0459817
GTCTACG1159.831803E-69.9499551
TGGACCG1002.6655442E-49.5321535
TAGGACG1002.6655442E-49.5321534
GTACCGT900.00112268759.492326
AAGTTCG800.0045662389.4878727
ATAGGAC3850.09.4083593
GTATCAA45950.09.4004891
TTACACG1252.6491445E-59.15086654
AAGACCG1151.0714187E-49.1177125
TACACGG1151.0714187E-49.1177125
ATGTCGC1054.5323273E-49.0403056
TAACCCG1901.0002623E-89.030465
CCGGTAC850.00723615248.9714383
Produced by FastQC (version 0.11.5)