FastQCFastQC Report
Wed 25 May 2016
SRR1294790_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294790_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464131
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC120380.48852922186361036No Hit
CCCATGTACTCTGCGTTGATACCAC118050.47907355574845656No Hit
GAGTACATGGGAAGCAGTGGTATCA57790.23452486901061673No Hit
CATGTACTCTGCGTTGATACCACTG52700.21386849968609623No Hit
GCGTTGATACCACTGCTTCCCATGT40960.16622492878828277No Hit
ACGCAGAGTACATGGGAAGCAGTGG31180.12653548045943985No Hit
GTATCAACGCAGAGTACATGGGAAG30000.12174677401485554No Hit
ACTCTGCGTTGATACCACTGCTTCC26550.10774589500314716No Hit
ATTCCATTCCATTCCATTCCATTCC25010.10149622727038457No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT500.001498201713.30209354
CGACCGC701.0914529E-412.21422110
CGCCTAA802.8878741E-511.86696914
TACATCG1104.9619666E-711.229045
AGTCGCG700.0014925110.8566447
CAGTACT3450.010.7408224
TCGACCG803.773254E-410.6872249
TCTAGAC2100.010.4053413
TATACTG2650.010.3978635
CACGGGT1106.0373277E-610.3652684
TATTCCG1201.5215064E-610.2932875
GTCGCGC750.00264907310.1330738
TCGCGCT750.00264907310.1330739
ACGTCGC750.002660718510.1277166
CCGTGCG1054.1080093E-59.9521259
GTCCTAA2201.8189894E-129.93277551
CGGAACG1252.5935988E-69.87331814
GTATTAC1651.02118065E-89.7888221
GTCTAAG1752.570232E-99.772371
TAAGACA2151.0913936E-119.7224614
Produced by FastQC (version 0.11.5)