FastQCFastQC Report
Wed 25 May 2016
SRR1294790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464131
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC121090.49141056218196194No Hit
GTACATGGGAAGCAGTGGTATCAAC117450.47663862026815945No Hit
GAGTACATGGGAAGCAGTGGTATCA57990.23533651417071577No Hit
CATGTACTCTGCGTTGATACCACTG53640.2176832319385617No Hit
GCGTTGATACCACTGCTTCCCATGT43090.17486894974333753No Hit
ACGCAGAGTACATGGGAAGCAGTGG33030.13404319819035596No Hit
GTATCAACGCAGAGTACATGGGAAG30580.12410054497914275No Hit
ACTCTGCGTTGATACCACTGCTTCC26860.10900394500130066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCC456.827908E-414.759559
CCGGATA551.9090433E-413.8618944
CGGATAG701.0596883E-412.2529235
TCGTCCG701.1040466E-412.1992199
TATACCG657.8164536E-411.7292945
GCTCGTT1001.9588206E-611.386411
GCTCGAA1001.9588206E-611.386411
CGCGCCA1001.9597774E-611.38593910
GTGCTAG2300.010.77551651
GGTCTAG2001.8189894E-1210.4854071
GTCTAAT1105.8093774E-610.398751
GACGAAC1303.9073166E-710.21856513
CTAGACT1701.4770194E-910.0906424
TCGACCG951.6653197E-49.9876659
GTCTTAT1356.154878E-79.8852321
CTACACT4750.09.8314
GTATTAG1951.5279511E-109.7766031
TATACTG2750.09.7033245
TACACTG5150.09.6225765
GTATAGG3100.09.5321881
Produced by FastQC (version 0.11.5)