FastQCFastQC Report
Wed 25 May 2016
SRR1294789_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294789_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2517257
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC30440.12092527699793863No Hit
CCCATGTACTCTGCGTTGATACCAC27640.10980205835161051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGGG1700.012.2910412
TCGTCGT550.003073737512.0886048
GACGGAT802.8730252E-511.87297257
GCTCGGA907.5080006E-611.60520118
TAGGACG1354.7184585E-911.2615964
GTCTCGT855.3360076E-511.174349
CGTCGTA600.00588571611.0812219
GTCGTAC700.001490861910.8580991
GTCTAAG1752.0008883E-1110.8580981
CTCGTGG2100.010.85442411
GTACCGT700.001498057610.8518336
TGTACCG803.7651928E-410.6897175
AGCCTCG2850.010.000882
ACCGTCT1054.1155614E-59.9504828
CGCACTG800.00450936059.5019715
TCGTACC800.00451380679.5008362
ACGGATC800.0045241959.4981898
GCTCGCA800.00452642389.49762212
TGCGTAG800.00452642389.49762212
TAGACAG3450.09.3642615
Produced by FastQC (version 0.11.5)