FastQCFastQC Report
Wed 25 May 2016
SRR1294789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2517257
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71380.28356262391960774No Hit
GGTATCAACGCAGAGTACTTTTTTT45300.17995778738523718No Hit
TATCAACGCAGAGTACTTTTTTTTT40240.15985654226008705No Hit
GTACATGGGAAGCAGTGGTATCAAC31500.12513620977119144No Hit
CCCATGTACTCTGCGTTGATACCAC29220.11607873173060995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC456.603348E-414.8248122
GTCGTAC551.9055282E-413.8649261
TATACCG802.7785067E-511.9118435
TGCGCTG2450.011.2797875
AGGACCG1158.477746E-710.772455
GCGGGAC1355.4389602E-810.5883053
ACCGGGC909.638165E-510.5426668
CGGGACT1451.3766112E-810.5152844
GCGCTGA2900.010.4743276
CTAGACT2100.010.4370444
GACGTAC750.002575363710.1676131
ATGTCGC750.00266628110.1251836
ACCGAGT750.002675524410.1209598
CCGCCCT750.002675524410.1209599
ACACCGA856.644935E-410.0507336
GGACCGG1054.14342E-59.9443766
TCTAGAC2350.09.732233
GTATTAG3350.09.6744081
GTTCTAG2500.09.5321371
GTCCTAT1909.895302E-109.5321371
Produced by FastQC (version 0.11.5)