FastQCFastQC Report
Wed 25 May 2016
SRR1294788_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294788_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86821
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA66377.644463896983448No Hit
GTACATGGGAAGCAGTGGTATCAAC18672.1504014005828083No Hit
CCCATGTACTCTGCGTTGATACCAC16891.9453818776563274No Hit
GTATCAACGCAGAGTACTTTTTTTT10611.222054572050541No Hit
GAGTACATGGGAAGCAGTGGTATCA9701.117241220442059No Hit
GGTATCAACGCAGAGTACTTTTTTT8660.9974545328895082No Hit
CATGTACTCTGCGTTGATACCACTG8080.9306504186775089No Hit
AAACAAAAAAAAAAAAAAAAAAAAA6870.7912832148904068No Hit
ACGCAGAGTACATGGGAAGCAGTGG6730.7751580838737172No Hit
GCGTTGATACCACTGCTTCCCATGT6530.7521221824213036No Hit
ACTCTGCGTTGATACCACTGCTTCC6030.6945324287902697No Hit
TATCAACGCAGAGTACTTTTTTTTT5980.6887734534271662No Hit
GGTATCAACGCAGAGTACATGGGAA5810.6691929371926147No Hit
GTGGTATCAACGCAGAGTACATGGG5680.654219601248546No Hit
GTATCAACGCAGAGTACATGGGAAG5360.6173621589246842No Hit
TATCAACGCAGAGTACATGGGAAGC5080.5851118968913052No Hit
GCTTCCCATGTACTCTGCGTTGATA3860.44459289803158225No Hit
GTACTCTGCGTTGATACCACTGCTT3800.4376821275958582No Hit
GATACCACTGCTTCCCATGTACTCT3320.38239596411006554No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3170.36511903802075535No Hit
ATACCACTGCTTCCCATGTACTCTG3170.36511903802075535No Hit
AAAAACAAAAAAAAAAAAAAAAAAA3110.35820826758503127No Hit
GGGAAGCAGTGGTATCAACGCAGAG2850.3282615956968936No Hit
CTGCTTCCCATGTACTCTGCGTTGA2750.3167436449706868No Hit
CAGTGGTATCAACGCAGAGTACATG2720.31328825975282476No Hit
CATGGGAAGCAGTGGTATCAACGCA2600.2994667188813766No Hit
GCAGAGTACATGGGAAGCAGTGGTA2590.2983149238087559No Hit
GCAGTGGTATCAACGCAGAGTACAT2580.29716312873613526No Hit
TACCACTGCTTCCCATGTACTCTGC2430.27988620264682507No Hit
GTTGATACCACTGCTTCCCATGTAC2350.27067184206585965No Hit
CTTCCCATGTACTCTGCGTTGATAC2240.2580020962670322No Hit
ACATGGGAAGCAGTGGTATCAACGC2140.24648414554082537No Hit
CCACTGCTTCCCATGTACTCTGCGT2140.24648414554082537No Hit
ACACAAAAAAAAAAAAAAAAAAAAA2030.2338143997419979No Hit
ATGGGAAGCAGTGGTATCAACGCAG2010.23151080959675652No Hit
AAGCAGTGGTATCAACGCAGAGTAC1960.22575183423365316No Hit
GGTATCAACGCAGAGTACATGGGGG1930.22229644901579113No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1760.20271593278123953No Hit
GTGGTATCAACGCAGAGTACTTTTT1620.18659080176455006No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1590.18313541654668802No Hit
TGATACCACTGCTTCCCATGTACTC1530.17622464611096392No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA1520.17507285103834325No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1520.17507285103834325No Hit
CCATGTACTCTGCGTTGATACCACT1440.16585849045737783No Hit
TTCCCATGTACTCTGCGTTGATACC1370.15779592494903305No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1320.15203694958592967No Hit
ATCAACGCAGAGTACATGGGAAGCA1250.14397438407758492No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA1250.14397438407758492No Hit
GTACTGGTTCACTATCGGTCAGTCA1170.13476002349661947No Hit
ACACAAAAAACAAAAAAAAAAAAAA1090.12554566291565405No Hit
ATCAACGCAGAGTACTTTTTTTTTT1050.12093848262517133No Hit
AAAAAAAACAAAAAAAAAAAAAAAA1010.11633130233468861No Hit
GGAAGCAGTGGTATCAACGCAGAGT1000.11517950726206794No Hit
GGTATCAACGCAGAGTACATGGGCA990.11402771218944725No Hit
GTACATGGTAAGCAGTGGTATCAAC980.11287591711682658No Hit
TCCATGTACTCTGCGTTGATACCAC950.10942053189896454No Hit
GGTATCAACGCAGAGTACATGGGGA930.10711694175372319No Hit
AAAAAGTACTCTGCGTTGATACCAC920.10596514668110249No Hit
CCTCTGGCCTTGCGGCCAATCGTTC870.10020617131799911No Hit
GTACATGGGGGATGGTGTTTCAGGG870.10020617131799911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA905.1933966E-712.6701291
GAAAAAA2405.0502604E-77.12694741
GAAGCAG5150.07.0075819
GGAAGCA5100.06.89006428
AGTGGTA5750.06.6066914
GGGAAGC5350.06.5680997
TGATACC5150.06.45435117
TTGATAC5150.06.45435116
AGCAGTG5950.06.384616411
CAAAAAA8050.06.37441254
CAGTGGT6000.06.33141113
AAGCAGT6000.06.33141110
CGTTGAT5400.06.155538614
TGCGTTG5250.06.150513612
TGGGAAG5800.06.0585056
CTGCGTT5351.8189894E-126.035550611
GTTGATA5550.05.98917315
ATGGGAA6350.05.98588755
GTGGTAT6350.05.982435715
ACAAAAA7500.05.95496033
Produced by FastQC (version 0.11.5)