##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294788_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86821
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.185646329805	33.0	30.0	34.0	16.0	34.0
2	29.47177526174543	33.0	30.0	34.0	16.0	34.0
3	29.965734096589536	33.0	30.0	34.0	18.0	34.0
4	32.43310950115755	37.0	35.0	37.0	17.0	37.0
5	33.55685836375992	37.0	35.0	37.0	27.0	37.0
6	33.23113071722279	37.0	35.0	37.0	21.0	37.0
7	33.430863500765945	37.0	35.0	37.0	23.0	37.0
8	33.25569850612179	37.0	35.0	37.0	21.0	37.0
9	34.40311675746651	39.0	35.0	39.0	17.0	39.0
10	34.78183849529492	39.0	35.0	39.0	21.0	39.0
11	34.65612006311837	39.0	35.0	39.0	18.0	39.0
12	34.928139505419196	39.0	35.0	39.0	23.0	39.0
13	34.551467962820055	39.0	35.0	39.0	18.0	39.0
14	35.87498416281775	41.0	36.0	41.0	19.0	41.0
15	35.8067057509128	40.0	36.0	41.0	18.0	41.0
16	35.80373411962544	40.0	36.0	41.0	19.0	41.0
17	35.85490837470197	40.0	36.0	41.0	21.0	41.0
18	35.74080003685744	40.0	36.0	41.0	21.0	41.0
19	35.697273701063104	40.0	35.0	41.0	21.0	41.0
20	35.64115824512503	40.0	35.0	41.0	21.0	41.0
21	35.59510947812165	40.0	35.0	41.0	21.0	41.0
22	35.67017196300434	40.0	35.0	41.0	22.0	41.0
23	35.70155837873325	40.0	34.0	41.0	24.0	41.0
24	35.85622142108476	40.0	34.0	41.0	25.0	41.0
25	35.05445687103351	40.0	33.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.8889258878095063
1101	2	-0.3834697684056714
1101	3	-0.4742711207613617
1101	4	-0.44708641558364093
1101	5	-0.15623265691123578
1101	6	-0.46611228955168826
1101	7	-0.5450101817488857
1101	8	-0.3708596024616071
1101	9	-0.14061054525016914
1101	10	-1.040440130165031
1101	11	-0.49727199271747224
1101	12	-0.6277681621153874
1101	13	-0.04881605010434242
1101	14	-0.7016327927294483
1101	15	-0.9093009511129395
1101	16	-0.7533222017665295
1101	17	-0.7043106854794061
1101	18	-1.3153245367547228
1101	19	-1.3235046066224854
1101	20	-1.70975447352793
1101	21	-0.9321176166463658
1101	22	-1.121604643549091
1101	23	-0.7874154512793368
1101	24	-1.0164260829795282
1101	25	-0.8454330705602118
1102	1	-0.7338180758618229
1102	2	-0.9436677182112554
1102	3	-1.030227289018697
1102	4	-1.0184543901329484
1102	5	-0.5237123317079835
1102	6	0.11239601727051252
1102	7	-1.154978368387276
1102	8	-1.0018246077638366
1102	9	-0.46934861523956073
1102	10	-0.830613336244916
1102	11	0.0291330974203845
1102	12	-0.6859512656855529
1102	13	0.08267917789141421
1102	14	-0.07745463790441676
1102	15	0.15043747235824867
1102	16	-0.36929957893161003
1102	17	-1.2620342627151828
1102	18	-0.7954942080166489
1102	19	-0.673592977071408
1102	20	-0.9780471564547568
1102	21	-0.40995430805676136
1102	22	0.30978453990795884
1102	23	-0.22721750147375275
1102	24	-0.6827392678505078
1102	25	-0.8484023176439877
1103	1	-1.1410998008529845
1103	2	-0.792165420579586
1103	3	-1.2047059033700585
1103	4	-1.3079559808010295
1103	5	-0.7475370047373247
1103	6	-0.36176446346473057
1103	7	-0.5710971382706234
1103	8	-1.283903080722478
1103	9	-0.9580018495979914
1103	10	-0.8230488258172031
1103	11	-0.09727199271747367
1103	12	-1.0190725099414735
1103	13	-0.45751170227825355
1103	14	-0.37119801012075015
1103	15	-0.431040081547728
1103	16	-0.5446265495926212
1103	17	-1.3217019898272326
1103	18	-0.3935854063199429
1103	19	-1.349591563144223
1103	20	-1.9358414300496705
1103	21	-1.6277697905594124
1103	22	-1.9476916000708329
1103	23	-1.6917632773662916
1103	24	-1.0338173873273533
1103	25	-1.445433070560206
1104	1	0.18175415678357965
1104	2	-0.3255730757263713
1104	3	-0.150302335923012
1104	4	-0.49896304137330816
1104	5	-0.9835830842616602
1104	6	-0.709367584980896
1104	7	-1.491647119690171
1104	8	-0.5388269008636897
1104	9	-0.8808558072345605
1104	10	-1.1220454813355971
1104	11	-0.31191338996756457
1104	12	-0.44434192651523574
1104	13	-1.2279316205242594
1104	14	-0.5952782776792773
1104	15	-0.985480809901496
1104	16	-0.5583760850812851
1104	17	-0.408584189752915
1104	18	0.15676762229395536
1104	19	-0.3129509091122671
1104	20	-0.28240404617750414
1104	21	-0.12394221716662202
1104	22	0.20117499227401936
1104	23	-0.007705306351802221
1104	24	-0.11676053114006635
1104	25	-0.5342476376356444
1105	1	0.5765018821764691
1105	2	0.49542223720442635
1105	3	0.41338666324985596
1105	4	0.1632922660432925
1105	5	0.6857028269597336
1105	6	0.10990454074283917
1105	7	-0.011980728733455237
1105	8	0.23047279585536273
1105	9	-0.23528095198228272
1105	10	0.08080811667929311
1105	11	0.4994320746037104
1105	12	-0.18765596015185082
1105	13	0.05356824162075924
1105	14	0.19752569254404762
1105	15	0.39224182588565526
1105	16	-0.1312324401957028
1105	17	-0.3317300403181207
1105	18	0.2536796708189115
1105	19	0.5761447622414693
1105	20	0.43540681679465365
1105	21	0.0074335754994976355
1105	22	0.32552018113533876
1105	23	0.3901441560137897
1105	24	0.39626676414529527
1105	25	1.028269594236427
1106	1	-1.3071735821441592
1106	2	-0.9131799133332095
1106	3	-1.491794177414853
1106	4	-1.7621720545823223
1106	5	-0.5865356872142655
1106	6	-1.3707894964028107
1106	7	-1.1959983240413763
1106	8	-1.4166435286803178
1106	9	-2.1192007955795447
1106	10	-1.7916259009160171
1106	11	-2.335611913666085
1106	12	-1.4806667128400264
1106	13	-1.7013193439119831
1106	14	-1.7829239653249687
1106	15	-1.5173378417585255
1106	16	-2.409580436285637
1106	17	-2.040674321843042
1106	18	-1.887327171800834
1106	19	-1.9712648174261744
1106	20	-1.7627847765582345
1106	21	-1.7371900804144857
1106	22	-1.435965644866613
1106	23	-0.7955840942305912
1106	24	-1.1738700882496218
1106	25	-1.8229034262914325
1107	1	-0.9849125098830847
1107	2	-0.9272824774357709
1107	3	-1.2374818231024989
1107	4	-0.9818690242792911
1107	5	-0.7485403492189278
1107	6	-0.5965470721603836
1107	7	0.2921135640705117
1107	8	-0.7200234820602702
1107	9	-1.0141891405678933
1107	10	-1.2605070197971386
1107	11	-1.1153321933863651
1107	12	0.010358928185617344
1107	13	-0.9039999965926384
1107	14	-0.010662893063894785
1107	15	-1.388899613320298
1107	16	-0.9182051449103454
1107	17	-0.19661837778710378
1107	18	-0.38254861702228027
1107	19	-0.11380560996696687
1107	20	-0.5482160119894672
1107	21	-0.2230875163119208
1107	22	-0.7466882555892269
1107	23	-0.8589873576338505
1107	24	-0.8552220696016022
1107	25	-0.5026237060117111
1108	1	-0.4022914272620035
1108	2	-1.1261428762961714
1108	3	-0.014689800310478063
1108	4	-0.14112828353854923
1108	5	-0.13401043468901008
1108	6	-1.3743248499381622
1108	7	-0.8454932735363272
1108	8	-0.1747243367611233
1108	9	-0.6894028157815697
1108	10	-0.2365753958655148
1108	11	-0.8389789170330886
1108	12	-0.8528889350622464
1108	13	-1.3789287715214158
1108	14	-1.461375143776145
1108	15	-1.1917486161692992
1108	16	-1.2267521534573547
1108	17	-0.6524588336275556
1108	18	-0.720155454629122
1108	19	-0.572779968941326
1108	20	-1.2727174364908933
1108	21	-1.372375265599672
1108	22	-1.2649218738228427
1108	23	-1.6151127137592098
1108	24	-1.13043574481528
1108	25	-0.8818259852139931
1109	1	-0.2106650182442884
1109	2	-0.08346976840567066
1109	3	-0.529343584529478
1109	4	-0.47462264746769733
1109	5	-0.012754396041671612
1109	6	-0.76901084027633
1109	7	-0.48124206580685325
1109	8	0.6726186584079556
1109	9	-0.6768424293081381
1109	10	-0.5897154924838688
1109	11	-0.11756184778993628
1109	12	-0.8973333795066907
1109	13	-0.744468224017389
1109	14	-0.18279221301930448
1109	15	0.054467164829084425
1109	16	0.1582720011320191
1109	17	-0.1217019898272369
1109	18	-0.16894772516052114
1109	19	-0.02495388198480697
1109	20	0.5801005989358359
1109	21	-0.2553060224434702
1109	22	0.5436127477552546
1109	23	0.20968599799602572
1109	24	0.3458927576001827
1109	25	-0.1120997372268775
1110	1	0.19030116533058816
1110	2	0.23682008666679266
1110	3	0.40012501450433646
1110	4	0.0810939386836722
1110	5	0.2882117875332071
1110	6	-0.49284336845668264
1110	7	-0.1936414216844753
1110	8	-0.2191687812055747
1110	9	0.036523110144358384
1110	10	0.36342460413448663
1110	11	0.42768774963357714
1110	12	-0.8602963424696526
1110	13	-0.0381880307806739
1110	14	-0.15026403266503507
1110	15	-0.5843412087618987
1110	16	0.002877476172280069
1110	17	-0.22282920399792516
1110	18	0.04280750833384417
1110	19	-0.1727799689413274
1110	20	0.12728256350910527
1110	21	0.13132843810403472
1110	22	0.3721151632141968
1110	23	0.04414654550005537
1110	24	-0.3822875966671262
1110	25	-0.16330746669547835
1111	1	0.33613449866392386
1111	2	0.6493200866667905
1111	3	0.6237361256154479
1111	4	-0.030480135390405394
1111	5	0.6659895653109871
1111	6	0.47011959450627927
1111	7	0.07672894868589708
1111	8	0.2613867743499867
1111	9	0.5411527397739917
1111	10	0.650924604134488
1111	11	0.7489840459298733
1111	12	1.0193332871599736
1111	13	0.47292308033043895
1111	14	1.0409396710386716
1111	15	0.3494550875344018
1111	16	0.5885256243204253
1111	17	0.1443004256317053
1111	18	0.5104001009264394
1111	19	1.0438866977253412
1111	20	0.9916344153609558
1111	21	0.53364325291885
1111	22	1.0248929409919683
1111	23	1.3117391380926477
1111	24	1.4056753662958315
1111	25	1.3589147555267473
1112	1	0.8002784895936266
1112	2	0.23818063088447872
1112	3	0.48130415282633265
1112	4	0.5861581866050614
1112	5	0.3893455743812808
1112	6	-0.04008448712637147
1112	7	-0.02871322818484856
1112	8	0.007135073669715553
1112	9	0.0720484313839691
1112	10	0.6074155338396992
1112	11	0.1149137511452949
1112	12	0.07545573613956691
1112	13	0.2008142367930219
1112	14	0.6459566778413901
1112	15	0.42669431655934176
1112	16	0.36871269915035754
1112	17	0.47664169547297064
1112	18	-0.2625590827470319
1112	19	-0.4612153430909842
1112	20	-0.36961841910616045
1112	21	-0.18446899197910938
1112	22	-0.6845741791894326
1112	23	-0.8444400002293477
1112	24	-0.3077600078538225
1112	25	-0.3927859247453611
1113	1	0.3816183102901185
1113	2	0.7352748328036576
1113	3	0.6533994810238362
1113	4	-0.01829286533889274
1113	5	0.45038985964505684
1113	6	0.47894961658133894
1113	7	0.5171262996792763
1113	8	0.45206006353321015
1113	9	0.4821939465436742
1113	10	1.2452494753633303
1113	11	0.4455440164964628
1113	12	0.963593772811187
1113	13	0.830538974370171
1113	14	0.5300401125397727
1113	15	0.9752369124056273
1113	16	0.3894362203469157
1113	17	1.0228416324013878
1113	18	0.9841859728911189
1113	19	0.9693833864670651
1113	20	1.0584574470018708
1113	21	0.962037292653946
1113	22	1.1616663037587145
1113	23	1.3190055104252423
1113	24	0.7563928055894351
1113	25	0.39533859658634896
1114	1	0.543325075316524
1114	2	0.4401956140929535
1114	3	0.42094076696566063
1114	4	-8.56366051444013E-4
1114	5	0.19060278612673898
1114	6	0.1578833075864523
1114	7	0.14002008792640197
1114	8	-0.1716301032871499
1114	9	0.08035808435907654
1114	10	-0.029543047061011407
1114	11	-0.1474646179238519
1114	12	0.07102105087305688
1114	13	0.2703914347608176
1114	14	0.2669417807433092
1114	15	-0.09367079150919722
1114	16	0.049759801535614656
1114	17	-0.1954499259857414
1114	18	0.504598413162725
1114	19	0.6103423939278727
1114	20	0.8092328682442229
1114	21	0.9790546453239131
1114	22	0.9129027862802985
1114	23	0.6230963814119121
1114	24	1.151983383173473
1114	25	1.219674249197631
1115	1	0.8887138637432876
1115	2	1.0873376850104783
1115	3	0.4810083823442284
1115	4	0.8286899819318876
1115	5	1.040040635014229
1115	6	1.7723970272184957
1115	7	0.769275532536831
1115	8	1.1570907080973996
1115	9	1.2196379019548047
1115	10	1.0862679443691334
1115	11	1.110181423431598
1115	12	0.8417970552759186
1115	13	0.5909355026906908
1115	14	0.10085167932024319
1115	15	0.9043636451603518
1115	16	0.9522678603452732
1115	17	0.43730422135288904
1115	18	1.115731363866395
1115	19	0.8466817287812418
1115	20	1.0355871413789046
1115	21	1.0728513274530087
1115	22	1.3355382135937646
1115	23	1.0088578406461295
1115	24	0.9587291965235778
1115	25	0.6228899108062507
1116	1	-1.426557072217303
1116	2	-1.4689270397699907
1116	3	-1.091062724959265
1116	4	-1.3370414380723936
1116	5	-1.0114050707043347
1116	6	-0.9827539687121103
1116	7	-0.7887882926934111
1116	8	-1.1003948348454173
1116	9	-0.8035790609922984
1116	10	-1.138491104677776
1116	11	-1.5296558008134227
1116	12	-1.642160965713586
1116	13	-1.2719045058764564
1116	14	-1.428169524363632
1116	15	-1.4175468281744088
1116	16	-0.897250237748537
1116	17	-1.1250003406518232
1116	18	-0.92551943930345
1116	19	-0.7819753712401791
1116	20	-0.7925130942175826
1116	21	-0.7917877815639116
1116	22	-0.7941683616900193
1116	23	-0.8467857661219114
1116	24	-0.6790947486466976
1116	25	-0.4307404168870477
1201	1	-0.833090647534906
1201	2	-0.2245834221051375
1201	3	-0.1887638743845521
1201	4	0.11462220379088706
1201	5	-1.3457063411217618
1201	6	-0.3632137388270493
1201	7	-0.07239385833164391
1201	8	-0.5215079624921231
1201	9	-0.14671275730203348
1201	10	-0.7968093139941672
1201	11	-0.10993408272509697
1201	12	-0.32715794091019745
1201	13	0.21853711541815812
1201	14	0.33589581138954827
1201	15	-0.07634900603284933
1201	16	0.42295544888182945
1201	17	0.06937352504690608
1201	18	0.07399659215450782
1201	19	-0.17102558297641224
1201	20	-0.5694035963349506
1201	21	0.4864941301118293
1201	22	0.31363563105045245
1201	23	-0.1217403940710966
1201	24	-0.0789737565111821
1201	25	-0.7200326128943075
1202	1	-0.49414327911385314
1202	2	-0.2645132466665423
1202	3	0.2899027922821169
1202	4	0.04613097572070757
1202	5	-0.30023265691123413
1202	6	-0.10854707216038406
1202	7	-0.2712710513141019
1202	8	-0.8529465589833478
1202	9	-0.1489583713371232
1202	10	-0.4463531736432884
1202	11	-0.549793731847906
1202	12	0.02266662049331103
1202	13	0.0649230803304377
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2210	15	-1.7075493089484155
2210	16	-1.8130031526579913
2210	17	-1.422296296990197
2210	18	-1.9281490597450244
2210	19	-1.7420845252962422
2210	20	-1.7529199411538272
2210	21	-1.6970222147070473
2210	22	-1.8037441493357633
2210	23	-2.0772496708601906
2210	24	-1.7349121636801854
2210	25	-1.6806535897969965
2211	1	0.5719436774078837
2211	2	0.6701534200001262
2211	3	0.5431201835864634
2211	4	0.21378314963375544
2211	5	0.501738357581516
2211	6	0.23823553653526375
2211	7	0.5767289486858971
2211	8	0.6436331511615805
2211	9	0.605766266344034
2211	10	0.9610091451972878
2211	11	0.621568586992673
2211	12	0.5012173451309891
2211	13	0.6975607614898607
2211	14	0.12155561306765605
2211	15	0.8008439764232875
2211	16	0.9046488127262222
2211	17	0.6753994594481298
2211	18	0.8455450284626664
2211	19	0.5257707556963567
2211	20	0.9061875554575778
2211	21	0.5707809340782717
2211	22	0.5943373854364182
2211	23	0.1444686066916816
2211	24	-0.096136227907067
2211	25	0.03282779900500543
2212	1	0.1351184658525888
2212	2	0.993419639239498
2212	3	0.7027350070472167
2212	4	1.0550437273985622
2212	5	0.8800089538270228
2212	6	0.6155334647523674
2212	7	0.2948497540550221
2212	8	0.4272145148421558
2212	9	0.16577988369643748
2212	10	0.25276092031644026
2212	11	0.9166760668098135
2212	12	0.29058608358055693
2212	13	0.3856747581827875
2212	14	0.020106337705335875
2212	15	0.8992779808975655
2212	16	0.08391153036069454
2212	17	-0.03853887339887052
2212	18	0.7181741501434402
2212	19	0.8844907245709805
2212	20	1.0956146539888465
2212	21	1.2991633871470398
2212	22	0.5495946561299263
2212	23	1.0840172887264998
2212	24	1.0401831962734605
2212	25	0.6911295206274204
2213	1	-0.3645328895034652
2213	2	0.1268633767100802
2213	3	-0.37274655836723625
2213	4	0.25839071598044683
2213	5	0.17753357685499793
2213	6	-0.5471964228097335
2213	7	0.8949107668677172
2213	8	-0.24889461093140142
2213	9	-0.57482850120725
2213	10	-0.8143531736432905
2213	11	-0.19924827730245198
2213	12	-0.3388918210651326
2213	13	0.09629970370706076
2213	14	0.2603660779650738
2213	15	-0.04764087206155665
2213	16	-0.017805544510743232
2213	17	-0.04716782833655486
2213	18	-0.45767349214715836
2213	19	0.10557500941365561
2213	20	-0.1318323956058549
2213	21	-0.27723337045777185
2213	22	0.11814690924593663
2213	23	0.10484880620231252
2213	24	-0.04832896270849574
2213	25	-0.3488774522654623
2214	1	0.1646067208861446
2214	2	-0.3840132466665409
2214	3	-0.5970972077178835
2214	4	-0.1943690242792897
2214	5	-0.11873265691123436
2214	6	0.1222029278396164
2214	7	0.6642289486858957
2214	8	-0.20944655898335185
2214	9	-0.46370837133711973
2214	10	-0.48310317364329336
2214	11	-0.7037937318479024
2214	12	-0.48483337950668925
2214	13	-0.46457691966956105
2214	14	-1.1111436622946655
2214	15	-0.9658226902433782
2214	16	-0.7114743756795718
2214	17	-1.0543106854794075
2214	18	-0.5541832324068992
2214	19	-0.256113302274656
2214	20	-0.04725447352792855
2214	21	-0.52885674708115
2214	22	-0.23899594789691747
2214	23	0.03812802698153206
2214	24	0.11400869962916715
2214	25	-0.7848352444732498
2215	1	0.4017008767303025
2215	2	0.7632270130737169
2215	3	0.6467339611132843
2215	4	0.8492998068895403
2215	5	0.8268842262056495
2215	6	0.1930633174500045
2215	7	0.6806250525820019
2215	8	0.6082482462114527
2215	9	0.944652018273267
2215	10	1.0817507224606047
2215	11	1.0150374369832633
2215	12	0.5961731139998037
2215	13	0.4079880153953752
2215	14	0.7928336104326092
2215	15	0.4393721149514249
2215	16	0.6705061438009423
2215	17	0.5891958080270854
2215	18	0.8734953390216731
2215	19	0.8718087756474162
2215	20	0.991544227770774
2215	21	1.0344549412305355
2215	22	0.6765884676874947
2215	23	0.40355010490360854
2215	24	0.5685541541746204
2215	25	0.3329407295527247
2216	1	0.7394872941345518
2216	2	0.2614485367729493
2216	3	0.23904928909740164
2216	4	0.7219526317716642
2216	5	0.8577800819422663
2216	6	-0.011833696364206503
2216	7	-0.13027742074085324
2216	8	0.6374483454752493
2216	9	0.7079078707010922
2216	10	0.752525807248432
2216	11	0.33983684140050485
2216	12	0.268271716034711
2216	13	0.4092288128145185
2216	14	0.7844375478964167
2216	15	0.3889543798203192
2216	16	0.4503886816452649
2216	17	0.8090651106989384
2216	18	1.123722818548515
2216	19	0.7779631308463593
2216	20	0.9399270551344898
2216	21	0.6646145904984664
2216	22	0.6304308036954396
2216	23	0.9179847148796227
2216	24	0.7318430945336232
2216	25	0.9799338638070054
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70.0
3	13.0
4	6.0
5	3.0
6	9.0
7	24.0
8	21.0
9	19.0
10	69.0
11	132.0
12	206.0
13	410.0
14	664.0
15	1018.0
16	1419.0
17	1594.0
18	1575.0
19	1437.0
20	1397.0
21	1181.0
22	1112.0
23	1003.0
24	947.0
25	833.0
26	789.0
27	705.0
28	690.0
29	583.0
30	706.0
31	844.0
32	925.0
33	1217.0
34	1474.0
35	2117.0
36	4040.0
37	10585.0
38	46984.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.91627885545777	30.835598765147676	11.057227111459245	19.19089526793531
2	13.868251038301798	34.08167974157822	29.382787263497924	22.667281956622055
3	18.012945506570826	40.545280428286276	23.454211904789375	17.98756216035352
4	11.951543120853763	31.756561869051055	25.586385924430346	30.70550908566484
5	10.427076241359813	48.453132392480875	27.308184954822927	13.811606411336388
6	24.45526167049243	44.51570484352831	14.525970954979064	16.503062531000197
7	18.501984126984127	47.17723329641934	17.46954595791805	16.851236618678477
8	21.618286452386716	43.96429436390686	17.108950627962496	17.308468555743925
9	21.760295305110162	31.283885107855575	16.65936094128504	30.29645864574922
10	15.860087906509927	42.16166953151137	23.345984795173216	18.632257766805488
11	23.218507455801454	38.802458742258764	15.884951159599128	22.09408264234065
12	19.35387135096481	43.10100229524458	21.929389511078305	15.61573684271231
13	24.255535172429706	35.92814371257485	19.562032005353455	20.254289109641988
14	18.91265227021041	39.779438907345885	20.798726467331118	20.509182355112586
15	26.361444671303826	36.68200851299443	19.328419328419326	17.628127487282416
16	16.55458792317896	40.246842378453195	24.830728415710247	18.367841282657594
17	19.761003967887792	40.4470794500323	21.841838147088676	17.950078434991234
18	19.322913662840993	35.826748947459485	25.57586942730261	19.27446796239691
19	18.81530689917653	39.87151984868406	26.316517888035428	14.996655364103983
20	21.02045289374416	35.10099553566279	25.23446422185564	18.64408734873741
21	19.580790660652696	36.76675856818208	25.889695109993426	17.762755661171802
22	20.95077732158509	36.02666420630161	22.480047977118605	20.542510494994694
23	18.81192687006171	36.015917873003055	25.566641674837072	19.605513582098162
24	20.419911172636557	38.623752667704906	23.22893234123551	17.727403818423028
25	19.662882298424467	35.05560704355885	23.693234476367007	21.588276181649675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6876.0
1	6876.0
2	4672.0
3	2468.0
4	2468.0
5	2468.0
6	2065.5
7	1663.0
8	1663.0
9	1663.0
10	1644.5
11	1626.0
12	1626.0
13	1626.0
14	1449.5
15	1273.0
16	1273.0
17	1273.0
18	1518.0
19	1763.0
20	1763.0
21	1763.0
22	2271.0
23	2779.0
24	2779.0
25	2779.0
26	3232.5
27	3686.0
28	3686.0
29	3686.0
30	4241.0
31	4796.0
32	4796.0
33	4796.0
34	5037.5
35	5279.0
36	5279.0
37	5279.0
38	4968.5
39	4658.0
40	4658.0
41	4658.0
42	5075.5
43	5493.0
44	5493.0
45	5493.0
46	10662.5
47	15832.0
48	15832.0
49	15832.0
50	14167.0
51	12502.0
52	12502.0
53	12502.0
54	9781.0
55	7060.0
56	7060.0
57	7060.0
58	5427.0
59	3794.0
60	3794.0
61	3794.0
62	3215.0
63	2636.0
64	2636.0
65	2636.0
66	2025.5
67	1415.0
68	1415.0
69	1415.0
70	1057.0
71	699.0
72	699.0
73	699.0
74	522.5
75	346.0
76	346.0
77	346.0
78	236.0
79	126.0
80	126.0
81	126.0
82	82.0
83	38.0
84	38.0
85	38.0
86	24.5
87	11.0
88	11.0
89	11.0
90	6.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010366155653586114
2	0.1624031052395158
3	0.1727692608931019
4	0.16816208060261917
5	0.18659080176455006
6	0.14742976929544696
7	0.15318874465855037
8	0.1290010481335161
9	0.150885154513309
10	0.15894772002165375
11	0.12669745798827473
12	0.1382154087144815
13	0.17046567074786054
14	0.15318874465855037
15	0.1497333594406883
16	0.1451261791502056
17	0.14397438407758492
18	0.1451261791502056
19	0.1324564333513781
20	0.15434053973117104
21	0.15434053973117104
22	0.13015284320613676
23	0.1451261791502056
24	0.15664412987641238
25	0.5770493313829603
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	86821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.217896591838375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.54782561248432	34.00559772405294
2	11.021989414525017	9.306504186775088
3	3.535766901293174	4.478179242349201
4	1.5878212473399902	2.6813789290609416
5	0.7366181044360779	1.5549233480379172
6	0.4665247994761827	1.181741744508817
7	0.27827795056474053	0.8223816818511651
8	0.20461614012113274	0.6910770435724076
9	0.16096469689529108	0.6116031835615807
>10	1.1731325366944945	9.395192407366881
>50	0.14459540568560048	4.25473099826079
>100	0.09821574725814373	8.781285633660058
>500	0.03273858241938124	9.27310212966909
>1k	0.00818464560484531	5.317837850289677
>5k	0.0027282152016151035	7.644463896983448
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	6637	7.644463896983448	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1867	2.1504014005828083	No Hit
CCCATGTACTCTGCGTTGATACCAC	1689	1.9453818776563274	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1061	1.222054572050541	No Hit
GAGTACATGGGAAGCAGTGGTATCA	970	1.117241220442059	No Hit
GGTATCAACGCAGAGTACTTTTTTT	866	0.9974545328895082	No Hit
CATGTACTCTGCGTTGATACCACTG	808	0.9306504186775089	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	687	0.7912832148904068	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	673	0.7751580838737172	No Hit
GCGTTGATACCACTGCTTCCCATGT	653	0.7521221824213036	No Hit
ACTCTGCGTTGATACCACTGCTTCC	603	0.6945324287902697	No Hit
TATCAACGCAGAGTACTTTTTTTTT	598	0.6887734534271662	No Hit
GGTATCAACGCAGAGTACATGGGAA	581	0.6691929371926147	No Hit
GTGGTATCAACGCAGAGTACATGGG	568	0.654219601248546	No Hit
GTATCAACGCAGAGTACATGGGAAG	536	0.6173621589246842	No Hit
TATCAACGCAGAGTACATGGGAAGC	508	0.5851118968913052	No Hit
GCTTCCCATGTACTCTGCGTTGATA	386	0.44459289803158225	No Hit
GTACTCTGCGTTGATACCACTGCTT	380	0.4376821275958582	No Hit
GATACCACTGCTTCCCATGTACTCT	332	0.38239596411006554	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	317	0.36511903802075535	No Hit
ATACCACTGCTTCCCATGTACTCTG	317	0.36511903802075535	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	311	0.35820826758503127	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	285	0.3282615956968936	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	275	0.3167436449706868	No Hit
CAGTGGTATCAACGCAGAGTACATG	272	0.31328825975282476	No Hit
CATGGGAAGCAGTGGTATCAACGCA	260	0.2994667188813766	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	259	0.2983149238087559	No Hit
GCAGTGGTATCAACGCAGAGTACAT	258	0.29716312873613526	No Hit
TACCACTGCTTCCCATGTACTCTGC	243	0.27988620264682507	No Hit
GTTGATACCACTGCTTCCCATGTAC	235	0.27067184206585965	No Hit
CTTCCCATGTACTCTGCGTTGATAC	224	0.2580020962670322	No Hit
ACATGGGAAGCAGTGGTATCAACGC	214	0.24648414554082537	No Hit
CCACTGCTTCCCATGTACTCTGCGT	214	0.24648414554082537	No Hit
ACACAAAAAAAAAAAAAAAAAAAAA	203	0.2338143997419979	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	201	0.23151080959675652	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	196	0.22575183423365316	No Hit
GGTATCAACGCAGAGTACATGGGGG	193	0.22229644901579113	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	176	0.20271593278123953	No Hit
GTGGTATCAACGCAGAGTACTTTTT	162	0.18659080176455006	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	159	0.18313541654668802	No Hit
TGATACCACTGCTTCCCATGTACTC	153	0.17622464611096392	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	152	0.17507285103834325	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	152	0.17507285103834325	No Hit
CCATGTACTCTGCGTTGATACCACT	144	0.16585849045737783	No Hit
TTCCCATGTACTCTGCGTTGATACC	137	0.15779592494903305	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	132	0.15203694958592967	No Hit
ATCAACGCAGAGTACATGGGAAGCA	125	0.14397438407758492	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA	125	0.14397438407758492	No Hit
GTACTGGTTCACTATCGGTCAGTCA	117	0.13476002349661947	No Hit
ACACAAAAAACAAAAAAAAAAAAAA	109	0.12554566291565405	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	105	0.12093848262517133	No Hit
AAAAAAAACAAAAAAAAAAAAAAAA	101	0.11633130233468861	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	100	0.11517950726206794	No Hit
GGTATCAACGCAGAGTACATGGGCA	99	0.11402771218944725	No Hit
GTACATGGTAAGCAGTGGTATCAAC	98	0.11287591711682658	No Hit
TCCATGTACTCTGCGTTGATACCAC	95	0.10942053189896454	No Hit
GGTATCAACGCAGAGTACATGGGGA	93	0.10711694175372319	No Hit
AAAAAGTACTCTGCGTTGATACCAC	92	0.10596514668110249	No Hit
CCTCTGGCCTTGCGGCCAATCGTTC	87	0.10020617131799911	No Hit
GTACATGGGGGATGGTGTTTCAGGG	87	0.10020617131799911	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0011517950726206794	0.0
13	0.0	0.0	0.0	0.0011517950726206794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACAAA	90	5.1933966E-7	12.670129	1
GAAAAAA	240	5.0502604E-7	7.1269474	1
GAAGCAG	515	0.0	7.007581	9
GGAAGCA	510	0.0	6.8900642	8
AGTGGTA	575	0.0	6.60669	14
GGGAAGC	535	0.0	6.568099	7
TGATACC	515	0.0	6.454351	17
TTGATAC	515	0.0	6.454351	16
AGCAGTG	595	0.0	6.3846164	11
CAAAAAA	805	0.0	6.3744125	4
CAGTGGT	600	0.0	6.331411	13
AAGCAGT	600	0.0	6.331411	10
CGTTGAT	540	0.0	6.1555386	14
TGCGTTG	525	0.0	6.1505136	12
TGGGAAG	580	0.0	6.058505	6
CTGCGTT	535	1.8189894E-12	6.0355506	11
GTTGATA	555	0.0	5.989173	15
ATGGGAA	635	0.0	5.9858875	5
GTGGTAT	635	0.0	5.9824357	15
ACAAAAA	750	0.0	5.9549603	3
>>END_MODULE