FastQCFastQC Report
Wed 25 May 2016
SRR1294788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86821
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36094.156828417088032No Hit
GGTATCAACGCAGAGTACTTTTTTT30713.537162668018106No Hit
TATCAACGCAGAGTACTTTTTTTTT24372.8069245919765953No Hit
GTACATGGGAAGCAGTGGTATCAAC18752.159615761163774No Hit
CCCATGTACTCTGCGTTGATACCAC18122.087052671588671No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15261.7576392808191565No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA13661.573352069199848No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11911.3717879314912291No Hit
GAGTACATGGGAAGCAGTGGTATCA9611.1068750647884729No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8701.002061713179991No Hit
CATGTACTCTGCGTTGATACCACTG8580.9882401723085429No Hit
ACGCAGAGTACATGGGAAGCAGTGG7040.8108637311249581No Hit
GCGTTGATACCACTGCTTCCCATGT6990.8051047557618549No Hit
GGTATCAACGCAGAGTACATGGGAA6140.7072021745890971No Hit
TATCAACGCAGAGTACATGGGAAGC5860.6749519125557181No Hit
GTGGTATCAACGCAGAGTACATGGG5820.6703447322652354No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5720.6588267815390286No Hit
ACTCTGCGTTGATACCACTGCTTCC5610.6461570357402011No Hit
GTGGTATCAACGCAGAGTACTTTTT5320.6127549786342015No Hit
GTATCAACGCAGAGTACATGGGAAG5240.603540618053236No Hit
ATCAACGCAGAGTACTTTTTTTTTT4630.5332811186233746No Hit
GCTTCCCATGTACTCTGCGTTGATA4260.4906647009364094No Hit
GTACTCTGCGTTGATACCACTGCTT3600.41464622614344454No Hit
CAGTGGTATCAACGCAGAGTACATG3500.4031282754172378No Hit
GATACCACTGCTTCCCATGTACTCT3350.38585134932792753No Hit
ATACCACTGCTTCCCATGTACTCTG3290.3789405788922035No Hit
GGGAAGCAGTGGTATCAACGCAGAG3030.34899390700406585No Hit
GCAGAGTACATGGGAAGCAGTGGTA2800.32250262033379024No Hit
CATGGGAAGCAGTGGTATCAACGCA2770.31904723511592814No Hit
CTGCTTCCCATGTACTCTGCGTTGA2640.30407389917185934No Hit
CTTCCCATGTACTCTGCGTTGATAC2440.28103799771944576No Hit
ACATGGGAAGCAGTGGTATCAACGC2430.27988620264682507No Hit
GTTGATACCACTGCTTCCCATGTAC2400.276430817428963No Hit
GCAGTGGTATCAACGCAGAGTACAT2340.26952004699323895No Hit
CAACGCAGAGTACTTTTTTTTTTTT2150.24763594061344607No Hit
TACCACTGCTTCCCATGTACTCTGC2130.2453323504682047No Hit
AACGCAGAGTACTTTTTTTTTTTTT2110.24302876032296336No Hit
CCACTGCTTCCCATGTACTCTGCGT2050.23611798988723925No Hit
GGTATCAACGCAGAGTACATGGGGG1990.22920721945151518No Hit
ATGGGAAGCAGTGGTATCAACGCAG1990.22920721945151518No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1920.22114465394317043No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1880.21653747365268772No Hit
AAGCAGTGGTATCAACGCAGAGTAC1850.21308208843482568No Hit
CCATGTACTCTGCGTTGATACCACT1770.20386772785386023No Hit
TCAACGCAGAGTACTTTTTTTTTTT1490.17161746582048124No Hit
TGATACCACTGCTTCCCATGTACTC1460.16816208060261917No Hit
ATCAACGCAGAGTACATGGGAAGCA1390.16009951509427442No Hit
TTCCCATGTACTCTGCGTTGATACC1230.14167079393234355No Hit
GTACTGGTTCACTATCGGTCAGTCA1220.14051899885972288No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1120.1290010481335161No Hit
GGAAGCAGTGGTATCAACGCAGAGT1010.11633130233468861No Hit
GGGTTTCCCCATTCGGAAATCGCCG980.11287591711682658No Hit
CCTCTGGCCTTGCGGCCAATCGTTC940.10826873682634386No Hit
CTGCGTTGATACCACTGCTTCCCAT940.10826873682634386No Hit
GGATACCACGTGTCCCGCCCTACTC930.10711694175372319No Hit
TGGGAAGCAGTGGTATCAACGCAGA910.10481335160848182No Hit
GTACATGGTAAGCAGTGGTATCAAC910.10481335160848182No Hit
CGCAGAGTACTTTTTTTTTTTTTTT890.10250976146324046No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT880.10135796639061977No Hit
TCAACGCAGAGTACATGGGAAGCAG870.10020617131799911No Hit
AAACAAAAAAAAAAAAAAAAAAAAA870.10020617131799911No Hit
GGTATCAACGCAGAGTACATGGGCA870.10020617131799911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGTA4900.07.7777652
GGTATCA10750.07.62220951
CCCATGT5200.06.96259451
TGATACC5750.06.766314517
TGTACTC5650.06.7453185
ATGTACT5700.06.68614864
ATACCAC6000.06.64636919
CAGTGGT5500.06.55627413
TTGATAC5950.06.53887516
GATACCA6100.06.53364618
AGTGGTA5550.06.497207614
CATGTAC5900.06.45949943
TGCGTTG6100.06.37808312
CTGCGTT6150.06.32622911
GTACTCT6200.06.2788286
TACTCTG6200.06.27521137
GAAGCAG5001.8189894E-126.2629669
GTATCAA15200.06.2055811
GGAAGCA5051.8189894E-126.2009578
CTCTGCG6300.06.17560439
Produced by FastQC (version 0.11.5)