FastQCFastQC Report
Wed 25 May 2016
SRR1294787_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294787_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3039291
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC52910.17408665376234128No Hit
CCCATGTACTCTGCGTTGATACCAC47540.15641805934344555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTG500.001497874413.3026455
ACCGGAC655.4498683E-513.1553773
TCGTGGG2450.012.01724512
CTACGAC1201.2746932E-711.0846223
CGACACG600.005885028711.0815149
TATACCG951.35826795E-511.0021895
TAGACCG700.001489637210.85930255
TACGACT700.001489637210.85930254
CGGACGT700.001494361210.85518210
GGACCGA700.001497243110.85267456
ATCGTCC1158.843799E-710.7373688
TCGTCGC1158.8456727E-710.73719110
ACCGGAA1605.475158E-1010.68468911
TTGCGTG1606.2427716E-910.087616
TCTATAC3600.010.0289463
CTACACG1054.0987376E-59.9543614
GCCTACG1054.100224E-59.9540331
ATCGTCG1151.0291762E-59.9114169
TCGTCCG1151.0291762E-59.9114169
ACACCGG1651.0260919E-89.7862729
Produced by FastQC (version 0.11.5)