FastQCFastQC Report
Wed 25 May 2016
SRR1294787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3039291
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87660.28842253012297936No Hit
GGTATCAACGCAGAGTACTTTTTTT53810.17704787070405564No Hit
GTACATGGGAAGCAGTGGTATCAAC53660.17655433454710326No Hit
TATCAACGCAGAGTACTTTTTTTTT50600.16648619694527442No Hit
CCCATGTACTCTGCGTTGATACCAC48790.16053086065138217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC1058.367351E-1114.5286345
GTCCTAG3450.011.0567721
TAGACAG4500.010.8056725
TACACCG1158.4235216E-710.7780355
TGTACCG803.648899E-410.7262195
CGGGTCT1252.2700078E-710.6305626
CACGGGT1553.4560799E-910.4571024
TACGACC1105.794638E-610.4011824
TCGTCCG1201.5526166E-610.2772929
CTACACT3750.010.1700444
ATCGTCC1356.5729364E-79.8380928
CGTCCGC1356.5729364E-79.83809210
TCCTATA3250.09.681262
CGGGACT2106.184564E-119.5344164
CACCGTT900.00112798019.4872017
ACCGTCT900.00112848939.48673258
TCGTTCA1106.94584E-59.48673213
TGCTCGT1651.08977474E-79.19925510
TCAGTAC2950.09.0496153
CTAATAC3800.09.0326053
Produced by FastQC (version 0.11.5)