FastQCFastQC Report
Wed 25 May 2016
SRR1294786_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294786_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3320140
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC143500.4322106899106664No Hit
CCCATGTACTCTGCGTTGATACCAC128990.38850771353015234No Hit
GAGTACATGGGAAGCAGTGGTATCA62100.1870403055292849No Hit
ATTCCATTCCATTCCATTCCATTCC53950.1624931478793063No Hit
CATGTACTCTGCGTTGATACCACTG45820.13800622865300863No Hit
GCGTTGATACCACTGCTTCCCATGT44250.13327751239405566No Hit
GAATGGAATGGAATGGAATGGAATG43530.13110892914154224No Hit
GTATCAACGCAGAGTACATGGGAAG40750.12273578824989308No Hit
ACGCAGAGTACATGGGAAGCAGTGG34090.10267639316414368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAT400.00529133314.24570911
GTCGCGC550.003074550512.0883638
TCGTCCG1103.830428E-812.0883639
ACGGGTC658.0204563E-411.6935535
TACCCCG752.0731144E-411.4012155
TACGACC855.3186133E-511.1781684
AACCGTG855.337459E-511.1742877
CACGGGT951.3590832E-511.001674
GTACGAC750.002647911410.13380053
CGCGATC750.002661873410.12737817
TTCGTCG856.6154264E-410.05579512
TAACCCG1803.947207E-1010.0288465
TCGTCGA900.00111728669.49713913
TCGTCTA1304.274809E-69.49427414
GACAATG5650.09.4140887
TAGACTG3050.09.3452585
AGACAAT5400.09.31985956
CTAGACT2252.7284841E-119.2902994
TTACACC2151.05501385E-109.2804784
GTCCCGC1856.693881E-99.2442281
Produced by FastQC (version 0.11.5)