FastQCFastQC Report
Wed 25 May 2016
SRR1294786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3320140
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC145040.43684904853409795No Hit
CCCATGTACTCTGCGTTGATACCAC132740.39980241797032656No Hit
GTATCAACGCAGAGTACTTTTTTTT110040.331431807092472No Hit
GGTATCAACGCAGAGTACTTTTTTT69370.20893697253730267No Hit
TATCAACGCAGAGTACTTTTTTTTT66490.20026263952724882No Hit
GAGTACATGGGAAGCAGTGGTATCA65670.19779286415633077No Hit
ATTCCATTCCATTCCATTCCATTCC51830.1561078749691278No Hit
CATGTACTCTGCGTTGATACCACTG48490.14604805821441263No Hit
GCGTTGATACCACTGCTTCCCATGT46080.1387893281608607No Hit
GAATGGAATGGAATGGAATGGAATG44800.1349340690452812No Hit
GTATCAACGCAGAGTACATGGGAAG43290.13038606805737107No Hit
ACGCAGAGTACATGGGAAGCAGTGG36750.11068810351370725No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33670.10141138626684418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC801.9214476E-613.1099833
CGGGACT2050.012.5576784
GTACCGT658.0845185E-411.6824696
TTACGCT600.0057213911.1236234
TACGACC951.3077026E-511.0399864
ATCGTCG1408.554707E-910.8414729
GTATTAG3500.010.6274121
TTGCGTG2151.8189894E-1210.1485816
GGACCGC750.002667486710.1248066
ACCGTCT1502.5034751E-810.1187088
CTCGCAT856.682549E-410.04475913
CGCGTCC856.685325E-410.04430510
GCGGGAC2105.456968E-129.9885593
TAGACTG4500.09.958295
CTAGACT2400.09.9318074
TTCGTCG1151.0444877E-59.89918312
GCTAGAC1553.846253E-89.8420993
TCGTCGC1554.1602107E-89.79229710
TAGGACC1856.0754246E-109.7922244
TACGCTG1106.574696E-59.5345345
Produced by FastQC (version 0.11.5)