FastQCFastQC Report
Wed 25 May 2016
SRR1294785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883139
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC48480.16815006144344757No Hit
CCCATGTACTCTGCGTTGATACCAC43910.15229928213658794No Hit
ATTCCATTCCATTCCATTCCATTCC29940.10384514933203012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG400.005288953414.2465859
TCGTCTA400.005303608314.24064314
ACCGGTC658.044396E-411.68930158
TAGACCG600.00587071711.0851085
CGACCGC700.001495057310.85454110
CTGGTCG803.7811438E-410.6849389
CTAGGAC2500.010.260933
TAGGACG856.5847655E-410.0607844
TCGTGGG1451.625449E-79.824717512
TTACTCG800.00451016339.5018524
CCGTACC1106.858082E-59.4977239
AGACTCG900.00111923119.4952476
CTGGACG1356.8344852E-69.1499324
GTGTTAG3950.09.1407041
TACATCG1054.487218E-49.04906755
CCGTAGA850.00742820328.9426091
AGCGCAA850.0074526078.939033510
GTCTAGA3700.08.7311281
AGACAGT5450.08.7112366
ACCGTCT1107.1652903E-48.6341448
Produced by FastQC (version 0.11.5)