FastQCFastQC Report
Wed 25 May 2016
SRR1294785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883139
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87330.3028990277610618No Hit
GGTATCAACGCAGAGTACTTTTTTT53880.18687964749531674No Hit
TATCAACGCAGAGTACTTTTTTTTT50910.1765783751667887No Hit
GTACATGGGAAGCAGTGGTATCAAC48770.16915590958327018No Hit
CCCATGTACTCTGCGTTGATACCAC44340.15379071213701456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGCG402.683921E-416.6877255
GCCGTTC400.00533160814.2293458
ATCGTCC707.37739E-613.5517588
TAGCCCG550.002984166812.1365275
GACCGTG1103.8962753E-812.0733837
TCGTCCG752.1031282E-411.3834769
CTGTCCG600.005941520411.0672679
CGTCCGC803.8236848E-410.672008510
GGACCGT1303.9007136E-710.22001656
CAATCCG856.6851336E-410.04424310
CATGACG951.658986E-49.991569519
GTGTTAC2106.184564E-119.5395011
GTCTAGG2900.09.5395011
GTCCCGC1002.6441316E-49.5395011
AGACCGT1201.722745E-59.4900156
ATACCGT1002.7859348E-49.4900156
GTCCGCA1106.949529E-59.4862311
CTAGACA3700.09.2781174
CTAAGAC2600.09.1690793
GACAGCG1252.803071E-59.1067817
Produced by FastQC (version 0.11.5)